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Annotation rule MF_03184
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General rule information [?]

Accession MF_03184
Dates 16-MAY-2014 (Created)
20-NOV-2019 (Last updated, Version 8)
Name Phosphofructokinase_I_E
Scope
Eukaryota
Templates P16861 (PFKA1_YEAST); P16862 (PFKA2_YEAST); P90521 (PFKA_DICDI); P17858 (PFKAL_HUMAN); P08237 (PFKAM_HUMAN); P00511 (PFKAM_RABIT): [Recover all]

Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
PFKA
Protein name
RecName: Full=ATP-dependent 6-phosphofructokinase;
Short=ATP-PFK;
Short=Phosphofructokinase;
EC 2.7.1.11;
AltName: Full=Phosphohexokinase;

Comments [?]

Function Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.
Catalytic activity RHEA:16109: ATP + beta-D-fructose 6-phosphate = ADP + beta-D-fructose 1,6-bisphosphate + H(+)
EC 2.7.1.11
Cofactor Mg(2+)
Activity regulation Allosterically activated by ADP, AMP, or fructose 2,6-bisphosphate, and allosterically inhibited by ATP or citrate.
Pathway Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4.
case <OC:Saccharomycetales>
Subunit Heterooctamer of 4 alpha and 4 beta chains.
else case <OC:Vertebrata>
Subunit Homo- and heterotetramers.
else
Subunit Homotetramer.
end case
Subcellular location Cytoplasm.
Similarity Belongs to the phosphofructokinase type A (PFKA) family. ATP-dependent PFK group I subfamily. Eukaryotic two domain clade 'E' sub-subfamily.

Keywords [?]


Gene Ontology [?]

GO:0003872; Molecular function: 6-phosphofructokinase activity.
GO:0006096; Biological process: glycolytic process.
GO:0005737; Cellular component: cytoplasm.

Cross-references [?]

Pfam PF00365; PFK; 2;
PIRSF PIRSF000533; ATP_PFK_euk; 1;
PRINTS PR00476; PHFRCTKINASE; 1;
TIGRFAMs TIGR02478; 6PF1K_euk; 1;
PROSITE PS00433; PHOSPHOFRUCTOKINASE; 1;

Features [?]

From: PFKA1_YEAST (P16861)
Key     From     To       Description   Tag   Condition   FTGroup
NP_BIND     278     279       ATP     R-x  
NP_BIND     308     311       ATP     G-[DE]-G-[ST]  
REGION     Nter     580       N-terminal catalytic PFK domain 1        
REGION     354     356       Substrate binding     S-x-D  
REGION     398     400       Substrate binding     M-G-R  
REGION     488     491       Substrate binding     H-x(2)-R  
REGION     581     594       Interdomain linker        
REGION     595     Cter       C-terminal regulatory PFK domain 2        
REGION     722     726       Allosteric activator fructose 2,6-bisphosphate binding     [TS]-[ILMV]-S-N-N  
REGION     767     769       Allosteric activator fructose 2,6-bisphosphate binding     [MQ]-G-[GA]  
REGION     859     862       Allosteric activator fructose 2,6-bisphosphate binding     H-x-Q-Q  
ACT_SITE     356     356       Proton acceptor     D  
METAL     309     309       Magnesium; catalytic     [DE]  
BINDING     215     215       ATP; via amide nitrogen     G  
BINDING     391     391       Substrate; shared with dimeric partner     R  
BINDING     455     455       Substrate     E  
BINDING     482     482       Substrate; shared with dimeric partner     [RK]  
BINDING     665     665       Allosteric activator fructose 2,6-bisphosphate     [RK]  
BINDING     760     760       Allosteric activator fructose 2,6-bisphosphate; shared with dimeric partner     R  
BINDING     827     827       Allosteric activator fructose 2,6-bisphosphate     [ED]  
BINDING     853     853       Allosteric activator fructose 2,6-bisphosphate; shared with dimeric partner     [RK]  
BINDING     952     952       Allosteric activator fructose 2,6-bisphosphate     R  

Additional information [?]

Size range 756-992 amino acids
Related rules None
Fusion None
Comments Classification of type A phosphofructokinases into subfamilies was done according to Mueller at al.(2001) (PubMed:11673446) and Bapteste et al.(2003) (PubMed:14585511).