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HAMAP rule MF_03190

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General rule information [?]

PURL https://purl.expasy.org/hamap/rule/MF_03190
Accession MF_03190
Dates 11-MAR-2015 (Created)
01-APR-2025 (Last updated, Version 22)
Name UbiG_COQ3_euk
Scope(s) Eukaryota
Template(s) P27680; Q9NZJ6; [ Recover all ]
Triggered by
case c? <OC:Eukaryota>
HAMAP; MF_00472 (Get profile general information and statistics)
end case

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier COQ3
Protein name RecName: Full=Ubiquinone biosynthesis O-methyltransferase, mitochondrial;
AltName: Full=3-demethylubiquinol 3-O-methyltransferase;
                 EC=2.1.1.64;
AltName: Full=3-demethylubiquinone 3-O-methyltransferase;
                 EC=2.1.1.-;
AltName: Full=Polyprenyldihydroxybenzoate methyltransferase;
                 EC=2.1.1.114;
case <OC:Saccharomycotina>
Protein name + AltName: Full=3,4-dihydroxy-5-hexaprenylbenzoate methyltransferase;
                 Short=DHHB methyltransferase;
                 Short=DHHB-MT;
                 Short=DHHB-MTase;
AltName: Full=3-demethylubiquinone-6 3-O-methyltransferase;
AltName: Full=Hexaprenyldihydroxybenzoate methyltransferase;
end case
case <OC:Vertebrata> or <OC:Fungi> or <OC:Viridiplantae>
Gene name Name=COQ3;
else case <OC:Drosophilidae>
Gene name Name=Coq3;
else case <OC:Insecta> or <OC:Dictyostelia>
Gene name Name=coq3;
else case <OC:Caenorhabditis>
Gene name Name=coq-3;
end case

Comments [?]

case <OC:Mammalia> or <OC:Schizosaccharomyces>
FUNCTIONO-methyltransferase required for two non-consecutive steps during ubiquinone biosynthesis. Catalyzes the 2 O-methylation of 3,4- dihydroxy-5-(all-trans-decaprenyl)benzoic acid into 4-hydroxy-3- methoxy-5-(all-trans-decaprenyl)benzoic acid. Also catalyzes the last step of ubiquinone biosynthesis by mediating methylation of 3- demethylubiquinone into ubiquinone. Also able to mediate the methylation of 3-demethylubiquinol-10 into ubiquinol-10.
CATALYTIC ACTIVITY Reaction=3,4-dihydroxy-5-(all-trans-decaprenyl)benzoate + S-adenosyl-L- methionine = 4-hydroxy-3-methoxy-5-(all-trans-decaprenyl)benzoate + S-adenosyl-L-homocysteine + H(+); Xref=Rhea:RHEA:44492, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789, ChEBI:CHEBI:62793, ChEBI:CHEBI:62796; EC=2.1.1.114;
CATALYTIC ACTIVITY Reaction=a 3-demethylubiquinone + S-adenosyl-L-methionine = a ubiquinone + S-adenosyl-L-homocysteine; Xref=Rhea:RHEA:81215, Rhea:RHEA-COMP:9565, Rhea:RHEA-COMP:19654, ChEBI:CHEBI:16389, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789, ChEBI:CHEBI:231825;
CATALYTIC ACTIVITY Reaction=3-demethylubiquinol-10 + S-adenosyl-L-methionine = ubiquinol- 10 + S-adenosyl-L-homocysteine + H(+); Xref=Rhea:RHEA:44412, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789, ChEBI:CHEBI:64182, ChEBI:CHEBI:64183; EC=2.1.1.64;
else case <OC:Saccharomyces>
FUNCTIONO-methyltransferase required for two non-consecutive steps during ubiquinone biosynthesis. Catalyzes the 2 O-methylation of 3,4- dihydroxy-5-(all-trans-hexaprenyl)benzoic acid into 4-hydroxy-3- methoxy-5-(all-trans-hexaprenyl)benzoic acid. Also catalyzes the last step of ubiquinone biosynthesis by mediating methylation of 3- demethylubiquinone into ubiquinone. Also able to mediate the methylation of 3-demethylubiquinol-6 into ubiquinol-6.
CATALYTIC ACTIVITY Reaction=3,4-dihydroxy-5-(all-trans-hexaprenyl)benzoate + S-adenosyl-L- methionine = 4-hydroxy-3-methoxy-5-(all-trans-hexaprenyl)benzoate + S-adenosyl-L-homocysteine + H(+); Xref=Rhea:RHEA:14121, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:57916, ChEBI:CHEBI:58373, ChEBI:CHEBI:59789; EC=2.1.1.114;
CATALYTIC ACTIVITY Reaction=a 3-demethylubiquinone + S-adenosyl-L-methionine = a ubiquinone + S-adenosyl-L-homocysteine; Xref=Rhea:RHEA:81215, Rhea:RHEA-COMP:9565, Rhea:RHEA-COMP:19654, ChEBI:CHEBI:16389, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789, ChEBI:CHEBI:231825;
CATALYTIC ACTIVITY Reaction=3-demethylubiquinol-6 + S-adenosyl-L-methionine = ubiquinol-6 + S-adenosyl-L-homocysteine + H(+); Xref=Rhea:RHEA:44400, ChEBI:CHEBI:15378, ChEBI:CHEBI:52970, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789, ChEBI:CHEBI:64253; EC=2.1.1.64;
else
FUNCTIONO-methyltransferase required for two non-consecutive steps during ubiquinone biosynthesis. Catalyzes the 2 O-methylation of 3,4- dihydroxy-5-(all-trans-polyprenyl)benzoic acid into 4-hydroxy-3- methoxy-5-(all-trans-polyprenyl)benzoic acid. Also catalyzes the last step of ubiquinone biosynthesis by mediating methylation of 3- demethylubiquinone into ubiquinone. Also able to mediate the methylation of 3-demethylubiquinol into ubiquinol.
CATALYTIC ACTIVITY Reaction=a 3,4-dihydroxy-5-(all-trans-polyprenyl)benzoate + S-adenosyl- L-methionine = a 4-hydroxy-3-methoxy-5-(all-trans-polyprenyl)benzoate + S-adenosyl-L-homocysteine + H(+); Xref=Rhea:RHEA:44452, Rhea:RHEA- COMP:10930, Rhea:RHEA-COMP:10931, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789, ChEBI:CHEBI:64694, ChEBI:CHEBI:84443; EC=2.1.1.114;
CATALYTIC ACTIVITY Reaction=a 3-demethylubiquinone + S-adenosyl-L-methionine = a ubiquinone + S-adenosyl-L-homocysteine; Xref=Rhea:RHEA:81215, Rhea:RHEA-COMP:9565, Rhea:RHEA-COMP:19654, ChEBI:CHEBI:16389, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789, ChEBI:CHEBI:231825;
CATALYTIC ACTIVITY Reaction=a 3-demethylubiquinol + S-adenosyl-L-methionine = a ubiquinol + S-adenosyl-L-homocysteine + H(+); Xref=Rhea:RHEA:44380, Rhea:RHEA- COMP:9566, Rhea:RHEA-COMP:10914, ChEBI:CHEBI:15378, ChEBI:CHEBI:17976, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789, ChEBI:CHEBI:84422; EC=2.1.1.64;
end case
COFACTOR Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
PATHWAYCofactor biosynthesis; ubiquinone biosynthesis.
case <OC:Saccharomyces>
SUBUNITComponent of a multi-subunit COQ enzyme complex, composed of at least @gn(COQ3), @gn(COQ4), @gn(COQ5), @gn(COQ6), @gn(COQ7) and @gn(COQ9). Interacts directly with COQ4.
else case <OC:Vertebrata> or <OC:Fungi> and not <OC:Saccharomyces>
SUBUNITComponent of a multi-subunit COQ enzyme complex, composed of at least @gn(COQ3), @gn(COQ4), @gn(COQ5), @gn(COQ6), @gn(COQ7) and @gn(COQ9).
else
SUBUNITComponent of a multi-subunit COQ enzyme complex.
end case
SUBCELLULAR LOCATIONMitochondrion inner membrane; Peripheral membrane protein; Matrix side.
case not <AnyFeature:TransitM>
MISCELLANEOUSThis protein may be expected to contain an N-terminal transit peptide but none has been predicted.
end case
SIMILARITYBelongs to the class I-like SAM-binding methyltransferase superfamily. UbiG/COQ3 family.

Keywords [?]

Magnesium
Membrane
Metal-binding
Methyltransferase
Mitochondrion
Mitochondrion inner membrane
S-adenosyl-L-methionine
Transferase
Ubiquinone biosynthesis

Gene Ontology [?]

GO:0031314; Cellular component:extrinsic component of mitochondrial inner membrane
GO:0061542; Molecular function:3-demethylubiquinol 3-O-methyltransferase activity
GO:0010420; Molecular function:polyprenyldihydroxybenzoate methyltransferase activity
GO:0006744; Biological process:ubiquinone biosynthetic process

Cross-references [?]

Pfam PF08241; Methyltransf_11; 0-1;
Pfam PF13847; Methyltransf_31; 0-1;
NCBIfam TIGR01983; UbiG; 1;
General TransitM; -; 0-1;

Features [?]

From: COQ3_YEAST (P27680)
Key From To Description Tag Condition FTGroup
BINDING 68 68 /ligand="S-adenosyl-L-methionine"
/ligand_id="ChEBI:CHEBI:59789"		 R
BINDING 130 130 /ligand="S-adenosyl-L-methionine"
/ligand_id="ChEBI:CHEBI:59789"		 G
BINDING 153 153 /ligand="S-adenosyl-L-methionine"
/ligand_id="ChEBI:CHEBI:59789"		 D
BINDING 196 196 /ligand="S-adenosyl-L-methionine"
/ligand_id="ChEBI:CHEBI:59789"
BINDING 197 197 /ligand="Mg(2+)"
/ligand_id="ChEBI:CHEBI:18420"		 E
BINDING 200 200 /ligand="Mg(2+)"
/ligand_id="ChEBI:CHEBI:18420"		 E
BINDING 201 201 /ligand="Mg(2+)"
/ligand_id="ChEBI:CHEBI:18420"		 H

Additional information [?]

Size range 232-371 amino acids
Related rules None
Fusion Nter: None Cter: None



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