HAMAP rule MF_03193
General rule information
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Accession | MF_03193 |
Dates | 20-MAR-2015 (Created) 7-JUL-2020 (Last updated, Version 8) |
Name | COQ6_monooxygenase |
Scope | Eukaryota |
Template | P53318 (COQ6_YEAST) |
Triggered by |
Propagated annotation
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Identifier, protein and gene names
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Identifier |
|
Protein name |
|
case <OC:Vertebrata>
Protein name |
|
end case
case <OC:Vertebrata> or <OC:Fungi> or <OC:Viridiplantae>
Gene name |
|
else case <OC:Drosophilidae>
Gene name |
|
else case <OC:Insecta> or <OC:Dictyostelia>
Gene name |
|
else case <OC:Caenorhabditis>
Gene name |
|
end case
Comments
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case <OC:Saccharomyces>
Function | FAD-dependent monooxygenase required for the C5-ring hydroxylation during ubiquinone biosynthesis. Catalyzes the hydroxylation of 3-hexaprenyl-4-hydroxybenzoic acid (HHB) to 3-hexaprenyl-4,5-dihydroxybenzoic acid (DHHB). The electrons required for the hydroxylation reaction may be funneled indirectly from NADPH via ferredoxin (YAH1) and ferredoxin reductase (ARH1) to COQ6. Can also convert 3-hexaprenyl-4-aminobenzoic acid (HAB), a COQ2-prenylated pABA, to DHHB in a two step process. HAB is first hydroxylated at C5 to yield 3-hexaprenyl-4-amino-5-hydroxybenzoic acid (HHAB) which is further deaminated at C4 by COQ6 to produce DHHB. |
Catalytic activity | RHEA:20361: 4-hydroxy-3-all-trans-hexaprenylbenzoate + 2 H(+) + O2 + 2 reduced [2Fe-2S]-[ferredoxin] = 3,4-dihydroxy-5-all-trans-hexaprenylbenzoate + H2O + 2 oxidized [2Fe-2S]-[ferredoxin] |
RHEA:58392: 4-amino-3-all-trans-hexaprenylbenzoate + 2 H(+) + O2 + 2 reduced [2Fe-2S]-[ferredoxin] = 4-amino-5-hydroxy-3-all-trans-hexaprenylbenzoate + H2O + 2 oxidized [2Fe-2S]-[ferredoxin] | |
RHEA:58396: 4-amino-5-hydroxy-3-all-trans-hexaprenylbenzoate + 5 H(+) + O2 + 4 reduced [2Fe-2S]-[ferredoxin] = 3,4-dihydroxy-5-all-trans-hexaprenylbenzoate + H2O + NH4(+) + 4 oxidized [2Fe-2S]-[ferredoxin] |
else
Function | FAD-dependent monooxygenase required for the C5-ring hydroxylation during ubiquinone biosynthesis. Catalyzes the hydroxylation of 3-polyprenyl-4-hydroxybenzoic acid to 3-polyprenyl-4,5-dihydroxybenzoic acid. The electrons required for the hydroxylation reaction may be funneled indirectly from NADPH via a ferredoxin/ferredoxin reductase system to COQ6. |
Catalytic activity | RHEA:20361: 4-hydroxy-3-all-trans-hexaprenylbenzoate + 2 H(+) + O2 + 2 reduced [2Fe-2S]-[ferredoxin] = 3,4-dihydroxy-5-all-trans-hexaprenylbenzoate + H2O + 2 oxidized [2Fe-2S]-[ferredoxin] |
end case
Cofactor | FAD |
Pathway | Cofactor biosynthesis; ubiquinone biosynthesis. |
case <OC:Vertebrata>
Subunit | Component of a multi-subunit COQ enzyme complex, composed of at least @gn(COQ3), @gn(COQ4), @gn(COQ5), @gn(COQ6), @gn(COQ7) and @gn(COQ9). Interacts with @gn(ADCK4) and @gn(COQ7). |
else case <OC:Fungi>
Subunit | Component of a multi-subunit COQ enzyme complex, composed of at least @gn(COQ3), @gn(COQ4), @gn(COQ5), @gn(COQ6), @gn(COQ7) and @gn(COQ9). |
else
Subunit | Component of a multi-subunit COQ enzyme complex. |
end case
case <OC:Vertebrata>
Subcellular location | Mitochondrion inner membrane; Peripheral membrane protein; Matrix side. Golgi apparatus. Cell projection. Note=Localizes to cell processes and Golgi apparatus in podocytes. |
else
Subcellular location | Mitochondrion inner membrane; Peripheral membrane protein; Matrix side. |
end case
case not <AnyFeature:TransitM>
Miscellaneous | This protein may be expected to contain an N-terminal transit peptide but none has been predicted. |
end case
Similarity | Belongs to the UbiH/COQ6 family. |
Keywords
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FAD
Flavoprotein
Membrane
Mitochondrion
Mitochondrion inner membrane
Monooxygenase
Oxidoreductase
Ubiquinone biosynthesis
Flavoprotein
Membrane
Mitochondrion
Mitochondrion inner membrane
Monooxygenase
Oxidoreductase
Ubiquinone biosynthesis
case <OC:Vertebrata>
end case
Gene Ontology
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GO:0031314; Cellular component: extrinsic component of mitochondrial inner membrane.
case <OC:Vertebrata>
end case
GO:0016712; Molecular function: oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen.
GO:0006744; Biological process: ubiquinone biosynthetic process.
GO:0006744; Biological process: ubiquinone biosynthetic process.
Cross-references
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PROSITE | PS01304; UBIH; 1; |
Pfam | PF01494; FAD_binding_3; 1; |
PRINTS | PR00420; RNGMNOXGNASE; 1; |
TIGRFAMs | TIGR01989; COQ6; 1; |
TIGR01988; Ubi-OHases; 1; |
Computed features
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General | TransitM; -; 0-1; trigger=yes; |
Additional information
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Size range | 451-605 amino acids |
Related rules | None |
Fusion | None |