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Annotation rule MF_03208
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General rule information [?]

Accession MF_03208
Dates 6-JAN-2016 (Created)
18-MAR-2020 (Last updated, Version 6)
Name PS_decarb_PSD_B_type1_euk
Scope
Eukaryota
Templates B3L2V1 (PSD_PLAKH); P39006 (PSD1_YEAST): [Recover all]

Propagated annotation [?]


Identifier, protein and gene names [?]

case <OC:Plasmodiidae>
Identifier
PSD
Protein name
RecName: Full=Phosphatidylserine decarboxylase proenzyme;
EC 4.1.1.65;
Contains:
RecName: Full=Phosphatidylserine decarboxylase beta chain;
Contains:
RecName: Full=Phosphatidylserine decarboxylase alpha chain;
Flags: Precursor;
else case <OC:Fungi> or <OC:Viridiplantae>
Identifier
PSD1
Protein name
RecName: Full=Phosphatidylserine decarboxylase proenzyme 1, mitochondrial;
EC 4.1.1.65;
Contains:
RecName: Full=Phosphatidylserine decarboxylase 1 beta chain;
Contains:
RecName: Full=Phosphatidylserine decarboxylase 1 alpha chain;
Flags: Precursor;
else
Identifier
PISD
Protein name
RecName: Full=Phosphatidylserine decarboxylase proenzyme, mitochondrial;
EC 4.1.1.65;
Contains:
RecName: Full=Phosphatidylserine decarboxylase beta chain;
Contains:
RecName: Full=Phosphatidylserine decarboxylase alpha chain;
Flags: Precursor;
end case
case <OC:Vertebrata>
Gene name
PISD
else case <OC:Caenorhabditis>
Gene name
psd-1
else case <OC:Fungi>
Gene name
PSD1
end case

Comments [?]

Function Catalyzes the formation of phosphatidylethanolamine (PtdEtn) from phosphatidylserine (PtdSer). Plays a central role in phospholipid metabolism and in the interorganelle trafficking of phosphatidylserine.
Catalytic activity RHEA:20828: a 1,2-diacyl-sn-glycero-3-phospho-L-serine + H(+) = a 1,2-diacyl-sn-glycero-3-phosphoethanolamine + CO2
EC 4.1.1.65
Cofactor pyruvate
Note: Binds 1 pyruvoyl group covalently per subunit.
case not <OC:Vertebrata>
Pathway Phospholipid metabolism; phosphatidylethanolamine biosynthesis; phosphatidylethanolamine from CDP-diacylglycerol: step 2/2.
end case
Subunit Heterodimer of a large membrane-associated beta subunit and a small pyruvoyl-containing alpha subunit.
case <OC:Plasmodiidae>
Subcellular location Endoplasmic reticulum membrane; Single-pass membrane protein. Note=Equally found in the membrane-bound as well as in the soluble fraction.
else case (<OC:Fungi> or <OC:Viridiplantae>)
Subcellular location Phosphatidylserine decarboxylase 1 beta chain: Mitochondrion inner membrane; Single-pass membrane protein; Intermembrane side.
Phosphatidylserine decarboxylase 1 alpha chain: Mitochondrion inner membrane; Peripheral membrane protein; Intermembrane side. Note=Anchored to the mitochondrial inner membrane through its interaction with the integral membrane beta chain.
else
Subcellular location Phosphatidylserine decarboxylase beta chain: Mitochondrion inner membrane; Single-pass membrane protein; Intermembrane side.
Phosphatidylserine decarboxylase alpha chain: Mitochondrion inner membrane; Peripheral membrane protein; Intermembrane side. Note=Anchored to the mitochondrial inner membrane through its interaction with the integral membrane beta chain.
end case
Ptm Is synthesized initially as an inactive proenzyme. Formation of the active enzyme involves a self-maturation process in which the active site pyruvoyl group is generated from an internal serine residue via an autocatalytic post-translational modification. Two non-identical subunits are generated from the proenzyme in this reaction, and the pyruvate is formed at the N-terminus of the alpha chain, which is derived from the carboxyl end of the proenzyme. The autoendoproteolytic cleavage occurs by a canonical serine protease mechanism, in which the side chain hydroxyl group of the serine supplies its oxygen atom to form the C-terminus of the beta chain, while the remainder of the serine residue undergoes an oxidative deamination to produce ammonia and the pyruvoyl prosthetic group on the alpha chain. During this reaction, the Ser that is part of the protease active site of the proenzyme becomes the pyruvoyl prosthetic group, which constitutes an essential element of the active site of the mature decarboxylase.
Similarity Belongs to the phosphatidylserine decarboxylase family. PSD-B subfamily. Eukaryotic type I sub-subfamily.
case not <AnyFeature:TransitM> and not <OC:Apicomplexa>
Miscellaneous This protein may be expected to contain an N-terminal transit peptide but none has been predicted.
end case

Keywords [?]

case <OC:Plasmodiidae>
else
end case

Gene Ontology [?]

case <OC:Plasmodiidae>
GO:0030176; Cellular component: integral component of endoplasmic reticulum membrane.
else
GO:0031305; Cellular component: integral component of mitochondrial inner membrane.
end case
GO:0004609; Molecular function: phosphatidylserine decarboxylase activity.
GO:0006646; Biological process: phosphatidylethanolamine biosynthetic process.
GO:0016540; Biological process: protein autoprocessing.

Cross-references [?]

Pfam PF02666; PS_Dcarbxylase; 1;
TIGRFAMs TIGR00163; PS_decarb; 1;

Computed features [?]

case not <OC:Apicomplexa>
General TransitM; -; 0-1; trigger=yes;
end case

Features [?]

case <OC:Fungi> or <OC:Viridiplantae>
From: PSD_PLAKH (B3L2V1)
Key     From     To       Description   Tag   Condition   FTGroup
CHAIN     Nter     307       Phosphatidylserine decarboxylase 1 beta chain        
CHAIN     308     Cter       Phosphatidylserine decarboxylase 1 alpha chain        
else
CHAIN     Nter     307       Phosphatidylserine decarboxylase beta chain        
CHAIN     308     Cter       Phosphatidylserine decarboxylase alpha chain        
end case
TRANSMEM     18     36       Helical        
case not <OC:Plasmodiidae>
TOPO_DOM     Nter     17       Mitochondrial matrix        
TOPO_DOM     37     Cter       Mitochondrial intermembrane        
end case
ACT_SITE     139     139       Charge relay system; for autoendoproteolytic cleavage activity     D  
ACT_SITE     198     198       Charge relay system; for autoendoproteolytic cleavage activity     H  
ACT_SITE     308     308       Charge relay system; for autoendoproteolytic cleavage activity     S  
ACT_SITE     308     308       Schiff-base intermediate with substrate; via pyruvic acid; for decarboxylase activity     S  
SITE     307     308       Cleavage (non-hydrolytic); by autocatalysis     G-S  
MOD_RES     308     308       Pyruvic acid (Ser); by autocatalysis     S  

Additional information [?]

Size range 336-655 amino acids
Related rules None
Fusion None
Comments Classification of phosphatidylserine decarboxylase into subfamilies was done according to Daiyasu at al.(2005) (PubMed:16243780) for PSD-A and PSD-B and Voelker D.R.(1997) (PubMed:9370338) for type I and type II.