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HAMAP rule MF_03212

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General rule information [?]

PURL https://purl.expasy.org/hamap/rule/MF_03212
Accession MF_03212
Dates 20-NOV-2015 (Created)
03-SEP-2024 (Last updated, Version 9)
Name NCPR
Scope(s) Eukaryota
Template(s) P00388; P16435; P16603; [ Recover all ]
Triggered by HAMAP; MF_03212 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier NCPR
Protein name RecName: Full=NADPH--cytochrome P450 reductase;
                 Short=CPR;
                 Short=P450R;
                 EC=1.6.2.4;
case <OC:Vertebrata>
Gene name Name=POR;
else case <OC:Saccharomycetales>
Gene name Name=NCP1;
else case <OC:Pezizomycotina>
Gene name Name=cprA;
end case

Comments [?]

case <OC:Fungi>
FUNCTIONThis enzyme is required for electron transfer from NADP to cytochrome P450 in microsomes. It can also provide electron transfer to heme oxygenase and cytochrome B5. Involved in ergosterol biosynthesis.
SUBCELLULAR LOCATIONEndoplasmic reticulum membrane; Single-pass membrane protein; Cytoplasmic side. Mitochondrion outer membrane; Single-pass membrane protein; Cytoplasmic side. Cell membrane; Single- pass membrane protein; Cytoplasmic side.
else
FUNCTIONThis enzyme is required for electron transfer from NADP to cytochrome P450 in microsomes. It can also provide electron transfer to heme oxygenase and cytochrome B5.
SUBCELLULAR LOCATIONEndoplasmic reticulum membrane; Single-pass membrane protein; Cytoplasmic side.
end case
CATALYTIC ACTIVITY Reaction=2 oxidized [cytochrome P450] + NADPH = 2 reduced [cytochrome P450] + NADP(+) + H(+); Xref=Rhea:RHEA:24040, Rhea:RHEA- COMP:14627, Rhea:RHEA-COMP:14628, ChEBI:CHEBI:15378, ChEBI:CHEBI:55376, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, ChEBI:CHEBI:60344; EC=1.6.2.4;
COFACTOR Name=FAD; Xref=ChEBI:CHEBI:57692; Note=Binds 1 FAD per monomer.;
COFACTOR Name=FMN; Xref=ChEBI:CHEBI:58210; Note=Binds 1 FMN per monomer.;
SIMILARITYBelongs to the NADPH--cytochrome P450 reductase family.
SIMILARITYIn the N-terminal section; belongs to the flavodoxin family.
SIMILARITYIn the C-terminal section; belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.

Keywords [?]

case <OC:Fungi>
Cell membrane
Mitochondrion
Mitochondrion outer membrane
Lipid biosynthesis
Lipid metabolism
Steroid biosynthesis
Steroid metabolism
Sterol biosynthesis
Sterol metabolism
end case
Endoplasmic reticulum
FAD
Flavoprotein
FMN
Membrane
NADP
Oxidoreductase

Gene Ontology [?]

case <OCellular component:Fungi>
GO:0005739; Cellular component:mitochondrion
GO:0005886; Cellular component:plasma membrane
GO:0006696; Biological process:ergosterol biosynthetic process
end case
GO:0005737; Cellular component:cytoplasm
GO:0005789; Cellular component:endoplasmic reticulum membrane
GO:0050660; Molecular function:flavin adenine dinucleotide binding
GO:0010181; Molecular function:FMN binding
GO:0050661; Molecular function:NADP binding
GO:0003958; Molecular function:NADPH-hemoprotein reductase activity

Cross-references [?]

PROSITE PS51384; FAD_FR; 1;
PROSITE PS50902; FLAVODOXIN_LIKE; 1;
Pfam PF00667; FAD_binding_1; 1;
Pfam PF00258; Flavodoxin_1; 1;
Pfam PF00175; NAD_binding_1; 1;
PRINTS PR00369; FLAVODOXIN; 1;
PRINTS PR00371; FPNCR; 1;
PIRSF PIRSF000208; P450R; 1;
General Transmembrane; -; 1;
ADD_TOPO_DOMAIN Lumenal; -; 1;
ADD_TOPO_DOMAIN Cytoplasmic; -; 1;

Features [?]

From: NCPR_RAT (P00388)
Key From To Description Tag Condition FTGroup
DOMAIN 80 224 /note="Flavodoxin-like"
BINDING 86 91 /ligand="FMN"
/ligand_id="ChEBI:CHEBI:58210"		 [ST]-Q-T-G-T-[AG]
BINDING 138 141 /ligand="FMN"
/ligand_id="ChEBI:CHEBI:58210"		 [AS]-T-Y-G
BINDING 173 182 /ligand="FMN"
/ligand_id="ChEBI:CHEBI:58210"		 L-G-[ND]-x-[TQ]-Y-E-x-[FY]-[NQ]
BINDING 454 457 /ligand="FAD"
/ligand_id="ChEBI:CHEBI:57692"		 R-[YF]-Y-S
BINDING 472 474 /ligand="FAD"
/ligand_id="ChEBI:CHEBI:57692"		 [CT]-x-[AVI]
BINDING 488 491 /ligand="FAD"
/ligand_id="ChEBI:CHEBI:57692"		 G-[VL]-x-[ST]
BINDING 596 597 /ligand="NADP(+)"
/ligand_id="ChEBI:CHEBI:58349"		 S-R
BINDING 602 606 /ligand="NADP(+)"
/ligand_id="ChEBI:CHEBI:58349"		 K-x-Y-V-[QT]
BINDING 208 208 /ligand="FMN"
/ligand_id="ChEBI:CHEBI:58210"		 D
BINDING 298 298 /ligand="NADP(+)"
/ligand_id="ChEBI:CHEBI:58349"		 R
BINDING 424 424 /ligand="FAD"
/ligand_id="ChEBI:CHEBI:57692"		 R
BINDING 478 478 /ligand="FAD"
/ligand_id="ChEBI:CHEBI:57692"		 Y
BINDING 535 535 /ligand="NADP(+)"
/ligand_id="ChEBI:CHEBI:58349"		 T
BINDING 639 639 /ligand="NADP(+)"
/ligand_id="ChEBI:CHEBI:58349"		 [DE]
BINDING 677 677 /ligand="FAD"
/ligand_id="ChEBI:CHEBI:57692"		 W

Additional information [?]

Size range 662-741 amino acids
Related rules None
Fusion Nter: None Cter: None



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