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HAMAP rule MF_03212

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General rule information [?]

Accession MF_03212
Dates 20-NOV-2015 (Created)
19-NOV-2022 (Last updated, Version 7)
Name NCPR
Scope
Eukaryota
Templates P00388 (NCPR_RAT); P16435 (NCPR_HUMAN); P16603 (NCPR_YEAST): [Recover all]
Triggered by

Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
NCPR
Protein name
RecName: Full=NADPH--cytochrome P450 reductase;
Short=CPR;
Short=P450R;
EC 1.6.2.4;
case <OC:Vertebrata>
Gene name
POR
else case <OC:Saccharomycetales>
Gene name
NCP1
else case <OC:Pezizomycotina>
Gene name
cprA
end case

Comments [?]

case <OC:Fungi>
Function This enzyme is required for electron transfer from NADP to cytochrome P450 in microsomes. It can also provide electron transfer to heme oxygenase and cytochrome B5. Involved in ergosterol biosynthesis.
Subcellular location Endoplasmic reticulum membrane; Single-pass membrane protein; Cytoplasmic side. Mitochondrion outer membrane; Single-pass membrane protein; Cytoplasmic side. Cell membrane; Single-pass membrane protein; Cytoplasmic side.
else
Function This enzyme is required for electron transfer from NADP to cytochrome P450 in microsomes. It can also provide electron transfer to heme oxygenase and cytochrome B5.
Subcellular location Endoplasmic reticulum membrane; Single-pass membrane protein; Cytoplasmic side.
end case
Catalytic activity RHEA:24040: NADPH + 2 oxidized [cytochrome P450] = H(+) + NADP(+) + 2 reduced [cytochrome P450]
EC 1.6.2.4
Cofactor FAD
Note: Binds 1 FAD per monomer.
FMN
Note: Binds 1 FMN per monomer.
Similarity Belongs to the NADPH--cytochrome P450 reductase family.
In the N-terminal section; belongs to the flavodoxin family.
In the C-terminal section; belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.

Keywords [?]

case <OC:Fungi>
Cell membrane
Mitochondrion
Mitochondrion outer membrane
Lipid biosynthesis
Lipid metabolism
Steroid biosynthesis
Steroid metabolism
Sterol biosynthesis
Sterol metabolism
end case
Endoplasmic reticulum
FAD
Flavoprotein
FMN
Membrane
NADP
Oxidoreductase

Gene Ontology [?]

case <OC:Fungi>
GO:0005739; Cellular component: mitochondrion.
GO:0005886; Cellular component: plasma membrane.
GO:0006696; Biological process: ergosterol biosynthetic process.
end case
GO:0005737; Cellular component: cytoplasm.
GO:0005789; Cellular component: endoplasmic reticulum membrane.
GO:0050660; Molecular function: flavin adenine dinucleotide binding.
GO:0010181; Molecular function: FMN binding.
GO:0050661; Molecular function: NADP binding.
GO:0003958; Molecular function: NADPH-hemoprotein reductase activity.

Cross-references [?]

PROSITE PS51384; FAD_FR; 1; trigger=PRU00716;
PS50902; FLAVODOXIN_LIKE; 1;
Pfam PF00667; FAD_binding_1; 1;
PF00258; Flavodoxin_1; 1;
PF00175; NAD_binding_1; 1;
PRINTS PR00369; FLAVODOXIN; 1;
PR00371; FPNCR; 1;
PIRSF PIRSF000208; P450R; 1;

Computed features [?]

General Transmembrane; -; 1; trigger=yes;
ADD_TOPO_DOMAIN Lumenal; -; 1; trigger=yes;
Cytoplasmic; -; 1; trigger=yes;

Features [?]

From: NCPR_RAT (P00388)
Key     From     To       Description   Tag   Condition   FTGroup
DOMAIN     80     224       Flavodoxin-like        
BINDING     86     91       /ligand="FMN" /ligand_id="ChEBI:CHEBI:58210     [ST]-Q-T-G-T-[AG]  
BINDING     138     141       /ligand="FMN" /ligand_id="ChEBI:CHEBI:58210     [AS]-T-Y-G  
BINDING     173     182       /ligand="FMN" /ligand_id="ChEBI:CHEBI:58210     L-G-[ND]-x-[TQ]-Y-E-x-[FY]-[NQ]  
BINDING     454     457       /ligand="FAD" /ligand_id="ChEBI:CHEBI:57692     R-[YF]-Y-S  
BINDING     472     474       /ligand="FAD" /ligand_id="ChEBI:CHEBI:57692     [CT]-x-[AVI]  
BINDING     488     491       /ligand="FAD" /ligand_id="ChEBI:CHEBI:57692     G-[VL]-x-[ST]  
BINDING     596     597       /ligand="NADP(+)" /ligand_id="ChEBI:CHEBI:58349     S-R  
BINDING     602     606       /ligand="NADP(+)" /ligand_id="ChEBI:CHEBI:58349     K-x-Y-V-[QT]  
BINDING     208     208       /ligand="FMN" /ligand_id="ChEBI:CHEBI:58210     D  
BINDING (Optional)     298     298       /ligand="NADP(+)" /ligand_id="ChEBI:CHEBI:58349     R  
BINDING (Optional)     424     424       /ligand="FAD" /ligand_id="ChEBI:CHEBI:57692     R  
BINDING (Optional)     478     478       /ligand="FAD" /ligand_id="ChEBI:CHEBI:57692     Y  
BINDING     535     535       /ligand="NADP(+)" /ligand_id="ChEBI:CHEBI:58349     T  
BINDING (Optional)     639     639       /ligand="NADP(+)" /ligand_id="ChEBI:CHEBI:58349     [DE]  
BINDING     677     677       /ligand="FAD" /ligand_id="ChEBI:CHEBI:57692     W  

Additional information [?]

Size range 662-741 amino acids
Related rules None
Fusion None


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