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HAMAP rule MF_04001

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General rule information [?]

Accession MF_04001
Dates 3-NOV-2015 (Created)
28-JUN-2022 (Last updated, Version 7)
Name PPV_E2
Viruses; Papillomaviridae
Template P03120 (VE2_HPV16)

Propagated annotation [?]

Identifier, protein and gene names [?]

Protein name
RecName: Full=Regulatory protein E2;
Gene name

Comments [?]

Function Plays a role in the initiation of viral DNA replication. A dimer of E2 interacts with a dimer of E1 in order to improve specificity of E1 DNA binding activity. Once the complex recognizes and binds DNA at specific sites, the E2 dimer is removed from DNA. E2 also regulates viral transcription through binding to the E2RE response element (5'-ACCNNNNNNGGT-3') present in multiple copies in the regulatory regions of the viral genome. Activates or represses transcription depending on E2RE's position with regards to proximal promoter elements including the TATA-box. Repression occurs by sterically hindering the assembly of the transcription initiation complex.
Subunit Binds DNA as homodimer. Interacts with protein E1; this interaction greatly increases E1 DNA-binding activity. Interacts with protein L1; this interaction enhances E2-dependent replication and transcription activation. Interacts with protein L2; this interaction inhibits E2 transcriptional activity but not DNA replication function E2. Interacts with protein E7; this interaction inhibits E7 oncogenic activity. Interacts with host TAF1; this interaction modulates E2-dependent transcriptional regulation. Interacts with host BRD4; this interaction mediates E2 transcriptional activation function. Additionally, the interaction with host BRD4 on mitotic chromosomes mediates tethering of the viral genome. Interacts with host TOPBP1; this interaction is required for optimal viral DNA replication.
Subcellular location Host nucleus.
Ptm Phosphorylated.
case <FTTag:Ubl>
Ptm Sumoylation plays a regulatory role in E2 transcriptional activity.
end case
Similarity Belongs to the papillomaviridae E2 protein family.

Keywords [?]

case <FTTag:Ubl>
end case

Gene Ontology [?]

GO:0042025; Cellular component: host cell nucleus.
GO:0003677; Molecular function: DNA binding.
GO:0000166; Molecular function: nucleotide binding.
GO:0003700; Molecular function: DNA-binding transcription factor activity.
GO:0006275; Biological process: regulation of DNA replication.
GO:0006351; Biological process: DNA-templated transcription.
GO:0039693; Biological process: viral DNA genome replication.

Cross-references [?]

Pfam PF00511; PPV_E2_C; 1;
PF00508; PPV_E2_N; 1;

Features [?]

From: VE2_HPV16 (P03120)
Key     From     To       Description   Tag   Condition   FTGroup
REGION     1     200       Transactivation domain        
REGION     285     Cter       DNA-binding domain        
CROSSLNK     292     292       Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)   Ubl   K  

Additional information [?]

Size range 310-514 amino acids
Related rules None
Fusion None