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HAMAP rule MF_04007

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General rule information [?]

Accession MF_04007
Dates 18-FEB-2016 (Created)
20-NOV-2019 (Last updated, Version 4)
Scope(s) Viruses
Template(s) P04296 (DNBI_HHV11); P36384 (DNBI_HHV2); [ Recover all ]
Triggered by HAMAP; MF_04007 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier DNBI
Protein name RecName: Full=Major DNA-binding protein;
Gene name Name=DBP;

Comments [?]

FUNCTIONPlays several crucial roles in viral infection. Participates in the opening of the viral DNA origin to initiate replication by interacting with the origin-binding protein. May disrupt loops, hairpins and other secondary structures present on ssDNA to reduce and eliminate pausing of viral DNA polymerase at specific sites during elongation. Promotes viral DNA recombination by performing strand- transfer, characterized by the ability to transfer a DNA strand from a linear duplex to a complementary single-stranded DNA circle. Can also catalyze the renaturation of complementary single strands. Additionally, reorganizes the host cell nucleus, leading to the formation of prereplicative sites and replication compartments. This process is driven by the protein which can form double-helical filaments in the absence of DNA.
SUBUNITHomooligomers. Forms double-helical filaments necessary for the formation of replication compartments within the host nucleus. Interacts with the origin-binding protein. Interacts with the helicase primase complex; this interaction stimulates primer synthesis activity of the helicase-primase complex. Interacts with the DNA polymerase. Interacts with the alkaline exonuclease; this interaction increases its nuclease processivity.
SUBCELLULAR LOCATIONHost nucleus. Note=In the absence of DNA replication, found in the nuclear framework-associated structures (prereplicative sites). As viral DNA replication proceeds, it migrates to globular intranuclear structures (replication compartments).
SIMILARITYBelongs to the herpesviridae major DNA-binding protein family.

Keywords [?]

Gene Ontology [?]

case <FTTag:Zn>
GO:0046872; Molecular function:metal ion binding
end case
GO:0042025; Cellular component:host cell nucleus
GO:0003697; Molecular function:single-stranded DNA binding
GO:0006260; Biological process:DNA replication
GO:0039686; Biological process:bidirectional double-stranded viral DNA replication

Cross-references [?]

Pfam PF00747; Viral_DNA_bp; 1;

Features [?]

From: DNBI_HHV11 (P04296)
Key From To Description Tag Condition FTGroup
ZN_FING 499 512 Zn C-x-x-C-x-x-x-x-x-x-x-C-x-H
MOTIF 843 844 /note="Required for filament formation" F-W
MOTIF 1142 1144 /note="Required for filament formation" F-N-F
REGION 1170 Cter /note="Required for nuclear localization"

Additional information [?]

Size range 1127-1209 amino acids
Related rules None
Fusion Nter: None Cter: None

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