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HAMAP rule MF_04047

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General rule information [?]

Accession MF_04047
Dates 13-FEB-2017 (Created)
20-NOV-2019 (Last updated, Version 4)
Viruses; Adenoviridae
Template P12537 (CAP3_ADE05)

Propagated annotation [?]

Identifier, protein and gene names [?]

Protein name
RecName: Full=Pre-hexon-linking protein IIIa;
AltName: Full=Capsid vertex-specific component IIIa;
AltName: Full=Protein IIIa;
AltName: Full=pIIIa;
RecName: Full=Hexon-linking protein IIIa;

Comments [?]

Function Structural component of the virion that acts as a cement protein on the capsid exterior which mediates the interactions between the hexons, including the peripentonal hexons, and reaches all the way to the penton vertices. Two hexon linking proteins IIIa, one from each facet, stabilize the unique edge interface between a pair of facets. As the virus enters the host cell, hexon linking proteins IIIa are shed concomitant with virion acidification in the endosome. During virus assembly, seems to play a role in the serotype specificity of the packaging of viral DNA via its interaction with packaging protein 3.
Subunit Interacts with hexon proteins; this interaction tethers the peripentonal hexons to hexons situated in the facet. Interacts with the penton protein (via N-terminus). Interacts with packaging protein 3; this interaction is required to promote correct genome packaging.
Subcellular location Virion. Host nucleus. Note=Surrounds the border of each facet on the capsid exterior. Present in around 60 copies per virion.
Induction Expressed in the late phase of the viral replicative cycle.
Ptm Cleaved near the C-terminus by the viral protease during virion maturation to form the mature protein.
Miscellaneous All late proteins expressed from the major late promoter are produced by alternative splicing and alternative polyadenylation of the same gene giving rise to non-overlapping ORFs. A leader sequence is present in the N-terminus of all these mRNAs and is recognized by the viral shutoff protein to provide expression although conventional translation via ribosome scanning from the cap has been shut off in the host cell.
Similarity Belongs to the adenoviridae hexon-linking protein IIIa family.

Keywords [?]

Gene Ontology [?]

GO:0042025; Cellular component: host cell nucleus.
GO:0019028; Cellular component: viral capsid.
GO:0098021; Cellular component: viral capsid, decoration.

Cross-references [?]

Pfam PF02455; Hex_IIIa; 1;

Features [?]

From: CAP3_ADE05 (P12537)
Key     From     To       Description   Tag   Condition   FTGroup
CHAIN     Nter     Cter       Pre-hexon-linking protein IIIa        
CHAIN     Nter     570       Hexon-linking protein IIIa        
PROPEP     571     Cter                
REGION     Nter     106       Peripentonal hexon-tethering domain        
REGION     138     251       Binding to hexon-linking protein        
SITE     570     571       Cleavage; by viral protease        
MOD_RES     225     225       Phosphoserine; by host     S  
MOD_RES     274     274       Phosphothreonine; by host     T  
MOD_RES     310     310       Phosphoserine; by host     S  
MOD_RES     444     444       Phosphoserine; by host     S  
MOD_RES     449     449       Phosphoserine; by host     S  
MOD_RES     450     450       Phosphoserine; by host     S  
MOD_RES     452     452       Phosphoserine; by host     S  
MOD_RES     469     469       Phosphoserine; by host     S  
MOD_RES     473     473       Phosphoserine; by host     S  
MOD_RES     490     490       Phosphotyrosine; by host     Y  
MOD_RES     494     494       Phosphoserine; by host     S  
MOD_RES     515     515       Phosphoserine; by host     S  

Additional information [?]

Size range 494-615 amino acids
Related rules None
Fusion None