HAMAP rule MF_04054

General rule information [?]

Accession	MF_04054
Dates	10-MAR-2017 (Created) 19-NOV-2022 (Last updated, Version 6)

Name

ADV_DNB2

Scope

Viruses; Adenoviridae

Template

P03265 (DNB2_ADE05)

Triggered by

HAMAP; MF_04054 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier

DNB2

Protein name

RecName:		Full=DNA-binding protein;
		Short=DBP;
AltName:		Full=Early 2A protein;
AltName:		Full=Early E2A DNA-binding protein;

Gene name

DBP

Comments [?]

Function	Plays a role in the elongation phase of viral strand displacement replication by unwinding the template in an ATP-independent fashion, employing its capacity to form multimers. Also enhances the rate of initiation. Released from template upon second strand synthesis. Assembles in complex with viral pTP, viral pol, host NFIA and host POU2F1/OCT1 on viral origin of replication. Covers the whole ssDNA genome during synthesis. The complementary strand synthesis induces its relese from DNA template. May inhibit cellular transcription mediated by the interaction between host SRCAP and CBP.
Subunit	Homomultimerizes on viral ssDNA bound to pTP. Forms a initiation complex with viral polymerase, pTP and hosts NFIA and POU2F1/OCT1. Interacts with host SRCAP.
Subcellular location	Host nucleus. Note=Accumulates in infected cells.
Domain	The C-terminal arm bridges DBP molecules together, thereby creating a chain.
Similarity	Belongs to the adenoviridae E2A DNA-binding protein family.

Keywords [?]

DNA replication
DNA-binding
Early protein
Host nucleus
Host-virus interaction
Metal-binding
Phosphoprotein
Viral DNA replication
Zinc

Gene Ontology [?]

GO:0042025; Cellular component: host cell nucleus.
GO:0019028; Cellular component: viral capsid.
GO:0003677; Molecular function: DNA binding.
GO:0008270; Molecular function: zinc ion binding.
GO:0032508; Biological process: DNA duplex unwinding.
GO:0045740; Biological process: positive regulation of DNA replication.
GO:0006351; Biological process: DNA-templated transcription.
GO:0039693; Biological process: viral DNA genome replication.
GO:0039687; Biological process: viral DNA strand displacement replication.

Cross-references [?]

Pfam	PF02236; Viral_DNA_bi; 1;
	PF03728; Viral_DNA_Zn_bi; 2;

Features [?]

From: DNB2_ADE05 (P03265)

Key From To Description Tag Condition FTGroup

REGION 297 331 Flexible loop

REGION 513 Cter C-terminal arm, DBP binding

BINDING 284 284 /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_label="1 C

BINDING 286 286 /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_label="1 H

BINDING 339 339 /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_label="1 C

BINDING 355 355 /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_label="1 C

BINDING 396 396 /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_label="2 C

BINDING 398 398 /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_label="2 C

BINDING 450 450 /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_label="2 C

BINDING 467 467 /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_label="2 C

MOD_RES 195 195 Phosphotyrosine; by host Y

Additional information [?]

Size range	397-529 amino acids
Related rules	None
Fusion	None

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