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HAMAP rule MF_04054

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General rule information [?]

Accession MF_04054
Dates 10-MAR-2017 (Created)
19-NOV-2022 (Last updated, Version 6)
Viruses; Adenoviridae
Template P03265 (DNB2_ADE05)

Propagated annotation [?]

Identifier, protein and gene names [?]

Protein name
RecName: Full=DNA-binding protein;
AltName: Full=Early 2A protein;
AltName: Full=Early E2A DNA-binding protein;
Gene name

Comments [?]

Function Plays a role in the elongation phase of viral strand displacement replication by unwinding the template in an ATP-independent fashion, employing its capacity to form multimers. Also enhances the rate of initiation. Released from template upon second strand synthesis. Assembles in complex with viral pTP, viral pol, host NFIA and host POU2F1/OCT1 on viral origin of replication. Covers the whole ssDNA genome during synthesis. The complementary strand synthesis induces its relese from DNA template. May inhibit cellular transcription mediated by the interaction between host SRCAP and CBP.
Subunit Homomultimerizes on viral ssDNA bound to pTP. Forms a initiation complex with viral polymerase, pTP and hosts NFIA and POU2F1/OCT1. Interacts with host SRCAP.
Subcellular location Host nucleus. Note=Accumulates in infected cells.
Domain The C-terminal arm bridges DBP molecules together, thereby creating a chain.
Similarity Belongs to the adenoviridae E2A DNA-binding protein family.

Keywords [?]

Gene Ontology [?]

GO:0042025; Cellular component: host cell nucleus.
GO:0019028; Cellular component: viral capsid.
GO:0003677; Molecular function: DNA binding.
GO:0008270; Molecular function: zinc ion binding.
GO:0032508; Biological process: DNA duplex unwinding.
GO:0045740; Biological process: positive regulation of DNA replication.
GO:0006351; Biological process: DNA-templated transcription.
GO:0039693; Biological process: viral DNA genome replication.
GO:0039687; Biological process: viral DNA strand displacement replication.

Cross-references [?]

Pfam PF02236; Viral_DNA_bi; 1;
PF03728; Viral_DNA_Zn_bi; 2;

Features [?]

From: DNB2_ADE05 (P03265)
Key     From     To       Description   Tag   Condition   FTGroup
REGION     297     331       Flexible loop        
REGION     513     Cter       C-terminal arm, DBP binding        
BINDING     284     284       /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_label="1     C  
BINDING     286     286       /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_label="1     H  
BINDING     339     339       /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_label="1     C  
BINDING     355     355       /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_label="1     C  
BINDING     396     396       /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_label="2     C  
BINDING     398     398       /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_label="2     C  
BINDING     450     450       /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_label="2     C  
BINDING     467     467       /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_label="2     C  
MOD_RES     195     195       Phosphotyrosine; by host     Y  

Additional information [?]

Size range 397-529 amino acids
Related rules None
Fusion None