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HAMAP rule MF_04054

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General rule information [?]

Accession MF_04054
Dates 10-MAR-2017 (Created)
19-NOV-2022 (Last updated, Version 6)
Scope(s) Viruses
Template(s) P03265 (DNB2_ADE05); [ Recover all ]
Triggered by HAMAP; MF_04054 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier DNB2
Protein name RecName: Full=DNA-binding protein;
AltName: Full=Early 2A protein;
AltName: Full=Early E2A DNA-binding protein;
Gene name Name=DBP;

Comments [?]

FUNCTIONPlays a role in the elongation phase of viral strand displacement replication by unwinding the template in an ATP- independent fashion, employing its capacity to form multimers. Also enhances the rate of initiation. Released from template upon second strand synthesis. Assembles in complex with viral pTP, viral pol, host NFIA and host POU2F1/OCT1 on viral origin of replication. Covers the whole ssDNA genome during synthesis. The complementary strand synthesis induces its relese from DNA template. May inhibit cellular transcription mediated by the interaction between host SRCAP and CBP.
SUBUNITHomomultimerizes on viral ssDNA bound to pTP. Forms a initiation complex with viral polymerase, pTP and hosts NFIA and POU2F1/OCT1. Interacts with host SRCAP.
SUBCELLULAR LOCATIONHost nucleus. Note=Accumulates in infected cells.
DOMAINThe C-terminal arm bridges DBP molecules together, thereby creating a chain.
SIMILARITYBelongs to the adenoviridae E2A DNA-binding protein family.

Keywords [?]

Gene Ontology [?]

GO:0042025; Cellular component:host cell nucleus
GO:0019028; Cellular component:viral capsid
GO:0003677; Molecular function:DNA binding
GO:0008270; Molecular function:zinc ion binding
GO:0032508; Biological process:DNA duplex unwinding
GO:0045740; Biological process:positive regulation of DNA replication
GO:0006351; Biological process:DNA-templated transcription
GO:0039693; Biological process:viral DNA genome replication
GO:0039687; Biological process:viral DNA strand displacement replication

Cross-references [?]

Pfam PF02236; Viral_DNA_bi; 1;
Pfam PF03728; Viral_DNA_Zn_bi; 2;

Features [?]

From: DNB2_ADE05 (P03265)
Key From To Description Tag Condition FTGroup
REGION 297 331 /note="Flexible loop"
REGION 513 Cter /note="C-terminal arm, DBP binding"
BINDING 284 284 /ligand="Zn(2+)"
BINDING 286 286 /ligand="Zn(2+)"
BINDING 339 339 /ligand="Zn(2+)"
BINDING 355 355 /ligand="Zn(2+)"
BINDING 396 396 /ligand="Zn(2+)"
BINDING 398 398 /ligand="Zn(2+)"
BINDING 450 450 /ligand="Zn(2+)"
BINDING 467 467 /ligand="Zn(2+)"
MOD_RES 195 195 /note="Phosphotyrosine; by host" Y

Additional information [?]

Size range 397-529 amino acids
Related rules None
Fusion Nter: None Cter: None

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