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HAMAP rule MF_04057

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General rule information [?]

Accession MF_04057
Dates 16-MAR-2017 (Created)
19-NOV-2022 (Last updated, Version 8)
Viruses; Adenoviridae
Template P03272 (PKG1_ADE02)

Propagated annotation [?]

Identifier, protein and gene names [?]

Protein name
RecName: Full=Packaging protein 1;
AltName: Full=Packaging protein IVa2;
Gene name

Comments [?]

Function Component of the packaging machinery which encapsidates the viral DNA into preformed capsids and transcriptional activator of the viral major late promoter (MLP). Binds, along with packaging proteins 2 and 3, to the specific packaging sequence on the left end of viral genomic DNA and displays ATPase activity thereby providing the power stroke of the packaging machinery. The activity of packaging protein IVa2 is stimulated by protein 33K which acts as a terminase. May be the protein that pumps DNA into the capsid powered by ATP hydrolysis. Specifically binds to the 5'-CG-3' nucleotides of the repeats making up the packaging sequence. Component of the DEF-A and DEF-B transcription factors that bind downstream elements of the major late promoter (MLP), and stimulate transcription from the MLP after initiation of viral DNA replication. DEF-A is a heterodimer packaging proteins 1 and 2 and DEF-B is a homodimer of packaging protein 1.
Subunit Homodimer. Part of a genome packaging complex composed of packaging proteins 1, 2 and 3; this complex specifically binds to the packaging sequence on the left end of viral genomic DNA and performs packaging of the viral genome. Interacts with protein 33K.
Subcellular location Virion. Host nucleus, host nucleoplasm. Host nucleus, host nucleolus. Note=Located at a unique vertex of the capsid. Present in about 6-8 copies per virion.
Induction Expressed in the intermediate phase of the viral replicative cycle.
Similarity Belongs to the adenoviridae packaging protein 1 family.

Keywords [?]

Gene Ontology [?]

GO:0044196; Cellular component: host cell nucleolus.
GO:0044095; Cellular component: host cell nucleoplasm.
GO:0044423; Cellular component: virion component.
GO:0005524; Molecular function: ATP binding.
GO:0003677; Molecular function: DNA binding.
GO:0039708; Biological process: nuclear capsid assembly.
GO:0006355; Biological process: regulation of DNA-templated transcription.
GO:0006351; Biological process: DNA-templated transcription.
GO:0019073; Biological process: viral DNA genome packaging.
GO:0098035; Biological process: viral DNA genome packaging via site-specific sequence recognition.
GO:0019076; Biological process: viral release from host cell.
GO:0019083; Biological process: viral transcription.

Cross-references [?]

Pfam PF02456; Adeno_IVa2; 1;

Features [?]

From: PKG1_ADE02 (P03272)
Key     From     To       Description   Tag   Condition   FTGroup
BINDING     171     178       /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616     G-P-x-x-x-x-K-S  
REGION     440     Cter       DNA-binding        

Additional information [?]

Size range 439-502 amino acids
Related rules None
Fusion None