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HAMAP rule MF_04057

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General rule information [?]

Accession MF_04057
Dates 16-MAR-2017 (Created)
19-NOV-2022 (Last updated, Version 8)
Scope(s) Viruses
Template(s) P03272 (PKG1_ADE02); [ Recover all ]
Triggered by HAMAP; MF_04057 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier PKG1
Protein name RecName: Full=Packaging protein 1;
AltName: Full=Packaging protein IVa2;
Gene name Name=IVa2;

Comments [?]

FUNCTIONComponent of the packaging machinery which encapsidates the viral DNA into preformed capsids and transcriptional activator of the viral major late promoter (MLP). Binds, along with packaging proteins 2 and 3, to the specific packaging sequence on the left end of viral genomic DNA and displays ATPase activity thereby providing the power stroke of the packaging machinery. The activity of packaging protein IVa2 is stimulated by protein 33K which acts as a terminase. May be the protein that pumps DNA into the capsid powered by ATP hydrolysis. Specifically binds to the 5'-CG-3' nucleotides of the repeats making up the packaging sequence. Component of the DEF-A and DEF-B transcription factors that bind downstream elements of the major late promoter (MLP), and stimulate transcription from the MLP after initiation of viral DNA replication. DEF-A is a heterodimer packaging proteins 1 and 2 and DEF- B is a homodimer of packaging protein 1.
SUBUNITHomodimer. Part of a genome packaging complex composed of packaging proteins 1, 2 and 3; this complex specifically binds to the packaging sequence on the left end of viral genomic DNA and performs packaging of the viral genome. Interacts with protein 33K.
SUBCELLULAR LOCATIONVirion. Host nucleus, host nucleoplasm. Host nucleus, host nucleolus. Note=Located at a unique vertex of the capsid. Present in about 6-8 copies per virion.
INDUCTIONExpressed in the intermediate phase of the viral replicative cycle.
SIMILARITYBelongs to the adenoviridae packaging protein 1 family.

Keywords [?]

Gene Ontology [?]

GO:0044196; Cellular component:host cell nucleolus
GO:0044095; Cellular component:host cell nucleoplasm
GO:0044423; Cellular component:virion component
GO:0005524; Molecular function:ATP binding
GO:0003677; Molecular function:DNA binding
GO:0039708; Biological process:nuclear capsid assembly
GO:0006355; Biological process:regulation of DNA-templated transcription
GO:0006351; Biological process:DNA-templated transcription
GO:0019073; Biological process:viral DNA genome packaging
GO:0098035; Biological process:viral DNA genome packaging via site-specific sequence recognition
GO:0019076; Biological process:viral release from host cell
GO:0019083; Biological process:viral transcription

Cross-references [?]

Pfam PF02456; Adeno_IVa2; 1;

Features [?]

From: PKG1_ADE02 (P03272)
Key From To Description Tag Condition FTGroup
BINDING 171 178 /ligand="ATP"
REGION 440 Cter /note="DNA-binding"

Additional information [?]

Size range 439-502 amino acids
Related rules None
Fusion Nter: None Cter: None

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