HAMAP rule MF_04057

General rule information [?]

Accession	MF_04057
Dates	16-MAR-2017 (Created) 19-NOV-2022 (Last updated, Version 8)

Name

ADV_PKG1

Scope

Viruses; Adenoviridae

Template

P03272 (PKG1_ADE02)

Triggered by

HAMAP; MF_04057 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier

PKG1

Protein name

RecName:		Full=Packaging protein 1;
AltName:		Full=Packaging protein IVa2;

Gene name

IVa2

Comments [?]

Function	Component of the packaging machinery which encapsidates the viral DNA into preformed capsids and transcriptional activator of the viral major late promoter (MLP). Binds, along with packaging proteins 2 and 3, to the specific packaging sequence on the left end of viral genomic DNA and displays ATPase activity thereby providing the power stroke of the packaging machinery. The activity of packaging protein IVa2 is stimulated by protein 33K which acts as a terminase. May be the protein that pumps DNA into the capsid powered by ATP hydrolysis. Specifically binds to the 5'-CG-3' nucleotides of the repeats making up the packaging sequence. Component of the DEF-A and DEF-B transcription factors that bind downstream elements of the major late promoter (MLP), and stimulate transcription from the MLP after initiation of viral DNA replication. DEF-A is a heterodimer packaging proteins 1 and 2 and DEF-B is a homodimer of packaging protein 1.
Subunit	Homodimer. Part of a genome packaging complex composed of packaging proteins 1, 2 and 3; this complex specifically binds to the packaging sequence on the left end of viral genomic DNA and performs packaging of the viral genome. Interacts with protein 33K.
Subcellular location	Virion. Host nucleus, host nucleoplasm. Host nucleus, host nucleolus. Note=Located at a unique vertex of the capsid. Present in about 6-8 copies per virion.
Induction	Expressed in the intermediate phase of the viral replicative cycle.
Similarity	Belongs to the adenoviridae packaging protein 1 family.

Keywords [?]

Activator
ATP-binding
DNA-binding
Host nucleus
Nucleotide-binding
Phosphoprotein
Transcription
Transcription regulation
Viral genome packaging
Virion
Viral release from host cell

Gene Ontology [?]

GO:0044196; Cellular component: host cell nucleolus.
GO:0044095; Cellular component: host cell nucleoplasm.
GO:0044423; Cellular component: virion component.
GO:0005524; Molecular function: ATP binding.
GO:0003677; Molecular function: DNA binding.
GO:0039708; Biological process: nuclear capsid assembly.
GO:0006355; Biological process: regulation of DNA-templated transcription.
GO:0006351; Biological process: DNA-templated transcription.
GO:0019073; Biological process: viral DNA genome packaging.
GO:0098035; Biological process: viral DNA genome packaging via site-specific sequence recognition.
GO:0019076; Biological process: viral release from host cell.
GO:0019083; Biological process: viral transcription.

Cross-references [?]

Pfam	PF02456; Adeno_IVa2; 1;

Features [?]

From: PKG1_ADE02 (P03272)

Key From To Description Tag Condition FTGroup

BINDING 171 178 /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616 G-P-x-x-x-x-K-S

REGION 440 Cter DNA-binding

Additional information [?]

Size range	439-502 amino acids
Related rules	None
Fusion	None

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