HAMAP rule MF_04061
General rule information
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| Accession | MF_04061 |
| Dates | 17-MAR-2017 (Created)
20-NOV-2019 (Last updated, Version 3) |
| Name | ADV_TERM |
| Scope(s) |
Viruses Adenoviridae |
| Template(s) | P04499 (TERM_ADE05); [ Recover all ] |
| Triggered by |
HAMAP; MF_04061 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | TERM |
| Protein name | RecName: Full=Preterminal protein; Short=pTP; AltName: Full=Bellett protein; AltName: Full=Precursor terminal protein; Contains: RecName: Full=Intermediate terminal protein; Short=iTP; Contains: RecName: Full=Terminal protein; Short=TP; |
| Gene name | Name=PTP; |
Comments
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| FUNCTION | Protein covalently bound to the viral DNA that acts as a primer for viral genomic replication by DNA strand displacement. Assembles on the viral origin of replication in an initiation complex with viral polymerase, DBP, host NFIA and host POU2F1/OCT1. During initiation, the polymerase covalently couples the first dCTP with Ser- 580 of pTP. The terminal protein stimulates the template activity over 20 fold compared to protein-free templates. Neo-synthesized viral genomes are linked to two preterminal proteins, one for each 5' end. These new genomes are encapsidated in the nucleus, and during capsid maturation by viral protease, preterminal protein is first cleaved into intermediary (iTP), then into mature TP. May play a role in host nuclear matrix localization of genomic DNA. |
| SUBUNIT | Heterodimer with the polymerase; this heterodimer binds to bp 9 to 18 of the genome. Interacts with host POU2F1; POU2F1 binds to the auxiliary sequences in the inverted terminal repeats and tethers the pTP-POL heterodimer to the origin DNA thereby participating in the assembly of the pre-initiation complex (POL-TP-DBP-NFIA-POU2F1). |
| SUBCELLULAR LOCATION | Host nucleus matrix. |
| PTM | Preterminal protein is used to replicate viral genome, upon genomic encapsidation it is processed first into iTP and finally into TP by adenovirus protease. |
| SIMILARITY | Belongs to the adenoviridae terminal protein family. |
Keywords
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| Covalent protein-DNA linkage |
| DNA replication |
| DNA-binding |
| Host nucleus |
| Phosphoprotein |
| Viral DNA replication |
Gene Ontology
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| GO:0044204; Cellular component:host cell nuclear matrix |
| GO:0042025; Cellular component:host cell nucleus |
| GO:0003677; Molecular function:DNA binding |
| GO:0003690; Molecular function:double-stranded DNA binding |
| GO:0003697; Molecular function:single-stranded DNA binding |
| GO:0039693; Biological process:viral DNA genome replication |
| GO:0039687; Biological process:viral DNA strand displacement replication |
Cross-references
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| Pfam | PF02459; Adeno_terminal; 1; |
Features
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| From: TERM_ADE05 (P04499) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| CHAIN | Nter | Cter | /note="Preterminal protein" | |||||||||
| CHAIN | 176 | Cter | /note="Intermediate terminal protein" | |||||||||
| CHAIN | 350 | Cter | /note="Terminal protein" | |||||||||
| MOTIF | 380 | 389 | /note="Nuclear localization signal" | |||||||||
| SITE | 175 | 176 | /note="Cleavage; by adenovirus protease" | x-G | ||||||||
| SITE | 349 | 350 | /note="Cleavage; by adenovirus protease" | |||||||||
| SITE | 580 | 580 | /note="Priming of strand displacement replication by covalently linking the first nucleotide of the new DNA chain" | S | ||||||||
| MOD_RES | 580 | 580 | /note="O-(5'-phospho-DNA)-serine" | S | ||||||||
Additional information
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| Size range | 575-723 amino acids |
| Related rules |
None |
| Fusion | Nter: None Cter: None |