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Annotation rule MF_04061
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General rule information [?]

Accession MF_04061
Dates 17-MAR-2017 (Created)
20-NOV-2019 (Last updated, Version 3)
Name ADV_TERM
Scope
Viruses; Adenoviridae
Template P04499 (TERM_ADE05)

Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
TERM
Protein name
RecName: Full=Preterminal protein;
Short=pTP;
AltName: Full=Bellett protein;
AltName: Full=Precursor terminal protein;
Contains:
RecName: Full=Intermediate terminal protein;
Short=iTP;
Contains:
RecName: Full=Terminal protein;
Short=TP;
Gene name
PTP

Comments [?]

Function Protein covalently bound to the viral DNA that acts as a primer for viral genomic replication by DNA strand displacement. Assembles on the viral origin of replication in an initiation complex with viral polymerase, DBP, host NFIA and host POU2F1/OCT1. During initiation, the polymerase covalently couples the first dCTP with Ser-580 of pTP. The terminal protein stimulates the template activity over 20 fold compared to protein-free templates. Neo-synthesized viral genomes are linked to two preterminal proteins, one for each 5' end. These new genomes are encapsidated in the nucleus, and during capsid maturation by viral protease, preterminal protein is first cleaved into intermediary (iTP), then into mature TP. May play a role in host nuclear matrix localization of genomic DNA.
Subunit Heterodimer with the polymerase; this heterodimer binds to bp 9 to 18 of the genome. Interacts with host POU2F1; POU2F1 binds to the auxiliary sequences in the inverted terminal repeats and tethers the pTP-POL heterodimer to the origin DNA thereby participating in the assembly of the pre-initiation complex (POL-TP-DBP-NFIA-POU2F1).
Subcellular location Host nucleus matrix.
Ptm Preterminal protein is used to replicate viral genome, upon genomic encapsidation it is processed first into iTP and finally into TP by adenovirus protease.
Similarity Belongs to the adenoviridae terminal protein family.

Keywords [?]


Gene Ontology [?]

GO:0044204; Cellular component: host cell nuclear matrix.
GO:0042025; Cellular component: host cell nucleus.
GO:0003677; Molecular function: DNA binding.
GO:0003690; Molecular function: double-stranded DNA binding.
GO:0003697; Molecular function: single-stranded DNA binding.
GO:0039693; Biological process: viral DNA genome replication.
GO:0039687; Biological process: viral DNA strand displacement replication.

Cross-references [?]

Pfam PF02459; Adeno_terminal; 1;

Features [?]

From: TERM_ADE05 (P04499)
Key     From     To       Description   Tag   Condition   FTGroup
CHAIN     Nter     Cter       Preterminal protein        
CHAIN     176     Cter       Intermediate terminal protein        
CHAIN     350     Cter       Terminal protein        
MOTIF     380     389       Nuclear localization signal        
SITE     175     176       Cleavage; by adenovirus protease     x-G  
SITE     349     350       Cleavage; by adenovirus protease        
SITE     580     580       Priming of strand displacement replication by covalently linking the first nucleotide of the new DNA chain     S  
MOD_RES     580     580       O-(5'-phospho-DNA)-serine     S  

Additional information [?]

Size range 575-723 amino acids
Related rules None
Fusion None