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HAMAP rule MF_04061

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General rule information [?]

Accession MF_04061
Dates 17-MAR-2017 (Created)
20-NOV-2019 (Last updated, Version 3)
Scope(s) Viruses
Template(s) P04499 (TERM_ADE05); [ Recover all ]
Triggered by HAMAP; MF_04061 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier TERM
Protein name RecName: Full=Preterminal protein;
AltName: Full=Bellett protein;
AltName: Full=Precursor terminal protein;
RecName: Full=Intermediate terminal protein;
RecName: Full=Terminal protein;
Gene name Name=PTP;

Comments [?]

FUNCTIONProtein covalently bound to the viral DNA that acts as a primer for viral genomic replication by DNA strand displacement. Assembles on the viral origin of replication in an initiation complex with viral polymerase, DBP, host NFIA and host POU2F1/OCT1. During initiation, the polymerase covalently couples the first dCTP with Ser- 580 of pTP. The terminal protein stimulates the template activity over 20 fold compared to protein-free templates. Neo-synthesized viral genomes are linked to two preterminal proteins, one for each 5' end. These new genomes are encapsidated in the nucleus, and during capsid maturation by viral protease, preterminal protein is first cleaved into intermediary (iTP), then into mature TP. May play a role in host nuclear matrix localization of genomic DNA.
SUBUNITHeterodimer with the polymerase; this heterodimer binds to bp 9 to 18 of the genome. Interacts with host POU2F1; POU2F1 binds to the auxiliary sequences in the inverted terminal repeats and tethers the pTP-POL heterodimer to the origin DNA thereby participating in the assembly of the pre-initiation complex (POL-TP-DBP-NFIA-POU2F1).
SUBCELLULAR LOCATIONHost nucleus matrix.
PTMPreterminal protein is used to replicate viral genome, upon genomic encapsidation it is processed first into iTP and finally into TP by adenovirus protease.
SIMILARITYBelongs to the adenoviridae terminal protein family.

Keywords [?]

Gene Ontology [?]

GO:0044204; Cellular component:host cell nuclear matrix
GO:0042025; Cellular component:host cell nucleus
GO:0003677; Molecular function:DNA binding
GO:0003690; Molecular function:double-stranded DNA binding
GO:0003697; Molecular function:single-stranded DNA binding
GO:0039693; Biological process:viral DNA genome replication
GO:0039687; Biological process:viral DNA strand displacement replication

Cross-references [?]

Pfam PF02459; Adeno_terminal; 1;

Features [?]

From: TERM_ADE05 (P04499)
Key From To Description Tag Condition FTGroup
CHAIN Nter Cter /note="Preterminal protein"
CHAIN 176 Cter /note="Intermediate terminal protein"
CHAIN 350 Cter /note="Terminal protein"
MOTIF 380 389 /note="Nuclear localization signal"
SITE 175 176 /note="Cleavage; by adenovirus protease" x-G
SITE 349 350 /note="Cleavage; by adenovirus protease"
SITE 580 580 /note="Priming of strand displacement replication by covalently linking the first nucleotide of the new DNA chain" S
MOD_RES 580 580 /note="O-(5'-phospho-DNA)-serine" S

Additional information [?]

Size range 575-723 amino acids
Related rules None
Fusion Nter: None Cter: None

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