HAMAP rule MF_04070
General rule information
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| Accession | MF_04070 |
| Dates | 13-APR-2017 (Created) 20-NOV-2019 (Last updated, Version 4) |
| Name | INFV_NCAP |
| Scope | Viruses; Orthomyxoviridae |
| Template | P03466 (NCAP_I34A1) |
| Triggered by |
Propagated annotation
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Identifier, protein and gene names
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| Identifier |
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| Protein name |
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| Gene name |
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Comments
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| Function | Encapsidates the negative strand viral RNA, protecting it from nucleases. The encapsidated genomic RNA is termed the ribonucleoprotein (RNP) and serves as template for transcription and replication. The RNP needs to be localized in the host nucleus to start an infectious cycle, but is too large to diffuse through the nuclear pore complex. NP comprises at least 2 nuclear localization signals that are responsible for the active RNP import into the nucleus through cellular importin alpha/beta pathway. Later in the infection, nclear export of RNPs are mediated through viral proteins NEP interacting with M1 which binds nucleoproteins. It is possible that nucleoprotein binds directly host exportin-1/XPO1 and plays an active role in RNPs nuclear export. M1 interaction with RNP seems to hide nucleoprotein's nuclear localization signals. Soon after a virion infects a new cell, M1 dissociates from the RNP under acidification of the virion driven by M2 protein. Dissociation of M1 from RNP unmasks nucleoprotein's nuclear localization signals, targeting the RNP to the nucleus. |
| Subunit | Homomultimerizes to form the nucleocapsid. May bind host exportin-1/XPO1. Binds to viral genomic RNA. Protein-RNA contacts are mediated by a combination of electrostatic interactions between positively charged residues and the phosphate backbone and planar interactions between aromatic side chains and bases. |
| Subcellular location | Virion. Host nucleus. |
| Ptm | Late in virus-infected cells, may be cleaved from a 56-kDa protein to a 53-kDa protein by a cellular caspase. This cleavage might be a marker for the onset of apoptosis in infected cells or have a specific function in virus host interaction. |
| Similarity | Belongs to the influenza viruses nucleoprotein family. |
Keywords
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Capsid protein
Helical capsid protein
Host nucleus
Host-virus interaction
Ribonucleoprotein
RNA-binding
Viral nucleoprotein
Viral penetration into host nucleus
Virion
Virus entry into host cell
Helical capsid protein
Host nucleus
Host-virus interaction
Ribonucleoprotein
RNA-binding
Viral nucleoprotein
Viral penetration into host nucleus
Virion
Virus entry into host cell
Gene Ontology
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GO:0019029; Cellular component: helical viral capsid.
GO:0042025; Cellular component: host cell nucleus.
GO:0019013; Cellular component: viral nucleocapsid.
GO:0003723; Molecular function: RNA binding.
GO:0005198; Molecular function: structural molecule activity.
GO:0075732; Biological process: viral penetration into host nucleus.
GO:0042025; Cellular component: host cell nucleus.
GO:0019013; Cellular component: viral nucleocapsid.
GO:0003723; Molecular function: RNA binding.
GO:0005198; Molecular function: structural molecule activity.
GO:0075732; Biological process: viral penetration into host nucleus.
Cross-references
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| Pfam | PF00506; Flu_NP; 1; |
Features
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case <OC:Alphainfluenzavirus>
| From: NCAP_I34A1 (P03466) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| MOTIF | 1 | 18 | Unconventional nuclear localization signal | |||||||||
| MOTIF | 198 | 216 | Bipartite nuclear localization signal | |||||||||
end case
Additional information
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| Size range | 498-565 amino acids |
| Related rules | None |
| Fusion | None |