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HAMAP rule MF_04070

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General rule information [?]

Accession MF_04070
Dates 13-APR-2017 (Created)
20-NOV-2019 (Last updated, Version 4)
Scope(s) Viruses
Template(s) P03466 (NCAP_I34A1); [ Recover all ]
Triggered by HAMAP; MF_04070 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier NCAP
Protein name RecName: Full=Nucleoprotein;
AltName: Full=Nucleocapsid protein;
                 Short=Protein N;
Gene name Name=NP;

Comments [?]

FUNCTIONEncapsidates the negative strand viral RNA, protecting it from nucleases. The encapsidated genomic RNA is termed the ribonucleoprotein (RNP) and serves as template for transcription and replication. The RNP needs to be localized in the host nucleus to start an infectious cycle, but is too large to diffuse through the nuclear pore complex. NP comprises at least 2 nuclear localization signals that are responsible for the active RNP import into the nucleus through cellular importin alpha/beta pathway. Later in the infection, nclear export of RNPs are mediated through viral proteins NEP interacting with M1 which binds nucleoproteins. It is possible that nucleoprotein binds directly host exportin-1/XPO1 and plays an active role in RNPs nuclear export. M1 interaction with RNP seems to hide nucleoprotein's nuclear localization signals. Soon after a virion infects a new cell, M1 dissociates from the RNP under acidification of the virion driven by M2 protein. Dissociation of M1 from RNP unmasks nucleoprotein's nuclear localization signals, targeting the RNP to the nucleus.
SUBUNITHomomultimerizes to form the nucleocapsid. May bind host exportin-1/XPO1. Binds to viral genomic RNA. Protein-RNA contacts are mediated by a combination of electrostatic interactions between positively charged residues and the phosphate backbone and planar interactions between aromatic side chains and bases.
PTMLate in virus-infected cells, may be cleaved from a 56-kDa protein to a 53-kDa protein by a cellular caspase. This cleavage might be a marker for the onset of apoptosis in infected cells or have a specific function in virus host interaction.
SIMILARITYBelongs to the influenza viruses nucleoprotein family.

Keywords [?]

Gene Ontology [?]

GO:0019029; Cellular component:helical viral capsid
GO:0042025; Cellular component:host cell nucleus
GO:0019013; Cellular component:viral nucleocapsid
GO:0003723; Molecular function:RNA binding
GO:0005198; Molecular function:structural molecule activity
GO:0075732; Biological process:viral penetration into host nucleus

Cross-references [?]

Pfam PF00506; Flu_NP; 1;

Features [?]

From: NCAP_I34A1 (P03466)
Key From To Description Tag Condition FTGroup
case <OC:Alphainfluenzavirus>
MOTIF 1 18 /note="Unconventional nuclear localization signal"
MOTIF 198 216 /note="Bipartite nuclear localization signal"
end case

Additional information [?]

Size range 498-565 amino acids
Related rules None
Fusion Nter: None Cter: None

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