HAMAP rule MF_04085

General rule information [?]

Accession	MF_04085
Dates	15-JAN-2018 (Created) 19-NOV-2022 (Last updated, Version 8)

Name	ARENA_NCAP

Scope

Viruses; Arenaviridae

Templates

P09992 (NCAP_LYCVA); P14239 (NCAP_JUNIN); P13699 (NCAP_LASSJ): [Recover all]

Triggered by

HAMAP; MF_04085 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier

NCAP

Protein name

RecName:		Full=Nucleoprotein;
		EC 3.1.13.-;
AltName:		Full=Nucleocapsid protein;
AltName:		Full=Protein N;

Gene name

Comments [?]

Function	Encapsidates the genome, protecting it from nucleases. The encapsidated genomic RNA is termed the nucleocapsid (NC). Serves as template for viral transcription and replication. The increased presence of protein N in host cell does not seem to trigger the switch from transcription to replication as observed in other negative strain RNA viruses. Through the interaction with host IKBKE, strongly inhibits the phosphorylation and nuclear translocation of host IRF3, a protein involved in interferon activation pathway, leading to the inhibition of interferon-beta and IRF3-dependent promoters activation. Encodes also a functional 3'-5' exoribonuclease that degrades preferentially dsRNA substrates and therby participates in the suppression of interferon induction.
Subunit	Homomultimerizes to form the nucleocapsid. Binds to viral genomic RNA. Interacts with glycoprotein G2. Interacts with protein Z; this interaction probably directs the encapsidated genome to budding sites. Interacts with protein L; this interaction does not interfere with Z-L interaction. Interacts with host IKBKE (via Protein kinase domain); the interaction inhibits IKBKE kinase activity.
Subcellular location	Virion. Host cytoplasm.
Domain	The N-terminal region is important for the cap-binding activity while the C-terminal region contains the 3'-5' exoribonuclease activity. A CCHE zinc binding site is present in the C-terminal region and may thus contribute to the substrate binding and/or the specificity of the exonuclease activity.
Similarity	Belongs to the arenaviridae nucleocapsid protein family.

Keywords [?]

Capsid protein
Helical capsid protein
Host cytoplasm
Host-virus interaction
Hydrolase
Inhibition of host IKBKE by virus
Inhibition of host innate immune response by virus
Inhibition of host RLR pathway by virus
Interferon antiviral system evasion
Ribonucleoprotein
RNA-binding
Viral immunoevasion
Viral nucleoprotein
Virion
Metal-binding
Zinc

Gene Ontology [?]

GO:0019029; Cellular component: helical viral capsid.
GO:0030430; Cellular component: host cell cytoplasm.
GO:0019013; Cellular component: viral nucleocapsid.
GO:0046872; Molecular function: metal ion binding.
GO:0003723; Molecular function: RNA binding.
GO:0039689; Biological process: negative stranded viral RNA replication.
GO:0039696; Biological process: RNA-templated viral transcription.
GO:0039724; Biological process: suppression by virus of host viral-induced cytoplasmic pattern recognition receptor signaling pathway via inhibition of IKBKE activity.

Cross-references [?]

Pfam	PF17290; Arena_ncap_C; 1;
	PF00843; Arena_nucleocap; 1;
PIRSF	PIRSF004029; N_ArenaV; 1;

Features [?]

From: NCAP_LASSJ (P13699)

Key From To Description Tag Condition FTGroup

REGION 54 241 Binding site for the cap structure m7GTP

BINDING 389 389 /ligand="Mn(2+)" /ligand_id="ChEBI:CHEBI:29035 D

BINDING 391 391 /ligand="Mn(2+)" /ligand_id="ChEBI:CHEBI:29035 E

BINDING 399 399 /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105 E

BINDING 506 506 /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105 C

BINDING 509 509 /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105 H

BINDING 529 529 /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105 C

BINDING 533 533 /ligand="Mn(2+)" /ligand_id="ChEBI:CHEBI:29035 D

SITE 466 466 Important for exonuclease activity D

Additional information [?]

Size range	557-570 amino acids
Related rules	None
Fusion	None

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