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Annotation rule MF_04085
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General rule information [?]

Accession MF_04085
Dates 15-JAN-2018 (Created)
20-NOV-2019 (Last updated, Version 5)
Name ARENA_NCAP
Scope
Viruses; Arenaviridae
Templates P09992 (NCAP_LYCVA); P14239 (NCAP_JUNIN); P13699 (NCAP_LASSJ): [Recover all]
Triggered by

Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
NCAP
Protein name
RecName: Full=Nucleoprotein;
EC 3.1.13.-;
AltName: Full=Nucleocapsid protein;
AltName: Full=Protein N;
Gene name
N

Comments [?]

Function Encapsidates the genome, protecting it from nucleases. The encapsidated genomic RNA is termed the nucleocapsid (NC). Serves as template for viral transcription and replication. The increased presence of protein N in host cell does not seem to trigger the switch from transcription to replication as observed in other negative strain RNA viruses. Through the interaction with host IKBKE, strongly inhibits the phosphorylation and nuclear translocation of host IRF3, a protein involved in interferon activation pathway, leading to the inhibition of interferon-beta and IRF3-dependent promoters activation. Encodes also a functional 3'-5' exoribonuclease that degrades preferentially dsRNA substrates and therby participates in the suppression of interferon induction.
Subunit Homomultimerizes to form the nucleocapsid. Binds to viral genomic RNA. Interacts with glycoprotein G2. Interacts with protein Z; this interaction probably directs the encapsidated genome to budding sites. Interacts with protein L; this interaction does not interfere with Z-L interaction. Interacts with host IKBKE (via Protein kinase domain); the interaction inhibits IKBKE kinase activity.
Subcellular location Virion. Host cytoplasm.
Domain The N-terminal region is important for the cap-binding activity while the C-terminal region contains the 3'-5' exoribonuclease activity. A CCHE zinc binding site is present in the C-terminal region and may thus contribute to the substrate binding and/or the specificity of the exonuclease activity.
Similarity Belongs to the arenaviridae nucleocapsid protein family.

Keywords [?]

Capsid protein
Helical capsid protein
Host cytoplasm
Host-virus interaction
Hydrolase
Inhibition of host IKBKE by virus
Inhibition of host innate immune response by virus
Inhibition of host RLR pathway by virus
Interferon antiviral system evasion
Ribonucleoprotein
RNA-binding
Viral immunoevasion
Viral nucleoprotein
Virion
Metal-binding
Zinc

Gene Ontology [?]

GO:0019029; Cellular component: helical viral capsid.
GO:0030430; Cellular component: host cell cytoplasm.
GO:0019013; Cellular component: viral nucleocapsid.
GO:0046872; Molecular function: metal ion binding.
GO:0003723; Molecular function: RNA binding.
GO:0039689; Biological process: negative stranded viral RNA replication.
GO:0039696; Biological process: RNA-templated viral transcription.
GO:0039724; Biological process: suppression by virus of host IKBKE activity.

Cross-references [?]

Pfam PF17290; Arena_ncap_C; 1;
PF00843; Arena_nucleocap; 1;
PIRSF PIRSF004029; N_ArenaV; 1;

Features [?]

From: NCAP_LASSJ (P13699)
Key     From     To       Description   Tag   Condition   FTGroup
REGION     54     241       Binding site for the cap structure m7GTP        
METAL     389     389       Manganese     D  
METAL     391     391       Manganese     E  
METAL     399     399       Zinc     E  
METAL     506     506       Zinc     C  
METAL     509     509       Zinc; via tele nitrogen     H  
METAL     529     529       Zinc     C  
METAL     533     533       Manganese     D  
SITE     466     466       Important for exonuclease activity     D  

Additional information [?]

Size range 557-570 amino acids
Related rules None
Fusion None


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