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HAMAP rule MF_04085

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General rule information [?]

Accession MF_04085
Dates 15-JAN-2018 (Created)
19-NOV-2022 (Last updated, Version 8)
Scope(s) Viruses
Template(s) P09992 (NCAP_LYCVA); P14239 (NCAP_JUNIN); P13699 (NCAP_LASSJ); [ Recover all ]
Triggered by HAMAP; MF_04085 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier NCAP
Protein name RecName: Full=Nucleoprotein;
AltName: Full=Nucleocapsid protein;
AltName: Full=Protein N;
Gene name Name=N;

Comments [?]

FUNCTIONEncapsidates the genome, protecting it from nucleases. The encapsidated genomic RNA is termed the nucleocapsid (NC). Serves as template for viral transcription and replication. The increased presence of protein N in host cell does not seem to trigger the switch from transcription to replication as observed in other negative strain RNA viruses. Through the interaction with host IKBKE, strongly inhibits the phosphorylation and nuclear translocation of host IRF3, a protein involved in interferon activation pathway, leading to the inhibition of interferon-beta and IRF3-dependent promoters activation. Encodes also a functional 3'-5' exoribonuclease that degrades preferentially dsRNA substrates and therby participates in the suppression of interferon induction.
SUBUNITHomomultimerizes to form the nucleocapsid. Binds to viral genomic RNA. Interacts with glycoprotein G2. Interacts with protein Z; this interaction probably directs the encapsidated genome to budding sites. Interacts with protein L; this interaction does not interfere with Z-L interaction. Interacts with host IKBKE (via Protein kinase domain); the interaction inhibits IKBKE kinase activity.
SUBCELLULAR LOCATIONVirion. Host cytoplasm.
DOMAINThe N-terminal region is important for the cap-binding activity while the C-terminal region contains the 3'-5' exoribonuclease activity. A CCHE zinc binding site is present in the C-terminal region and may thus contribute to the substrate binding and/or the specificity of the exonuclease activity.
SIMILARITYBelongs to the arenaviridae nucleocapsid protein family.

Keywords [?]

Gene Ontology [?]

GO:0019029; Cellular component:helical viral capsid
GO:0030430; Cellular component:host cell cytoplasm
GO:0019013; Cellular component:viral nucleocapsid
GO:0046872; Molecular function:metal ion binding
GO:0003723; Molecular function:RNA binding
GO:0039689; Biological process:negative stranded viral RNA replication
GO:0039696; Biological process:RNA-templated viral transcription
GO:0039724; Biological process:suppression by virus of host viral-induced cytoplasmic pattern recognition receptor signaling pathway via inhibition of IKBKE activity

Cross-references [?]

Pfam PF17290; Arena_ncap_C; 1;
Pfam PF00843; Arena_nucleocap; 1;
PIRSF PIRSF004029; N_ArenaV; 1;

Features [?]

From: NCAP_LASSJ (P13699)
Key From To Description Tag Condition FTGroup
REGION 54 241 /note="Binding site for the cap structure m7GTP"
BINDING 389 389 /ligand="Mn(2+)"
BINDING 391 391 /ligand="Mn(2+)"
BINDING 399 399 /ligand="Zn(2+)"
BINDING 506 506 /ligand="Zn(2+)"
BINDING 509 509 /ligand="Zn(2+)"
BINDING 529 529 /ligand="Zn(2+)"
BINDING 533 533 /ligand="Mn(2+)"
SITE 466 466 /note="Important for exonuclease activity" D

Additional information [?]

Size range 557-570 amino acids
Related rules None
Fusion Nter: None Cter: None

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