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HAMAP rule MF_04085

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General rule information [?]

Accession MF_04085
Dates 15-JAN-2018 (Created)
19-NOV-2022 (Last updated, Version 8)
Viruses; Arenaviridae
Templates P09992 (NCAP_LYCVA); P14239 (NCAP_JUNIN); P13699 (NCAP_LASSJ): [Recover all]

Propagated annotation [?]

Identifier, protein and gene names [?]

Protein name
RecName: Full=Nucleoprotein;
EC 3.1.13.-;
AltName: Full=Nucleocapsid protein;
AltName: Full=Protein N;
Gene name

Comments [?]

Function Encapsidates the genome, protecting it from nucleases. The encapsidated genomic RNA is termed the nucleocapsid (NC). Serves as template for viral transcription and replication. The increased presence of protein N in host cell does not seem to trigger the switch from transcription to replication as observed in other negative strain RNA viruses. Through the interaction with host IKBKE, strongly inhibits the phosphorylation and nuclear translocation of host IRF3, a protein involved in interferon activation pathway, leading to the inhibition of interferon-beta and IRF3-dependent promoters activation. Encodes also a functional 3'-5' exoribonuclease that degrades preferentially dsRNA substrates and therby participates in the suppression of interferon induction.
Subunit Homomultimerizes to form the nucleocapsid. Binds to viral genomic RNA. Interacts with glycoprotein G2. Interacts with protein Z; this interaction probably directs the encapsidated genome to budding sites. Interacts with protein L; this interaction does not interfere with Z-L interaction. Interacts with host IKBKE (via Protein kinase domain); the interaction inhibits IKBKE kinase activity.
Subcellular location Virion. Host cytoplasm.
Domain The N-terminal region is important for the cap-binding activity while the C-terminal region contains the 3'-5' exoribonuclease activity. A CCHE zinc binding site is present in the C-terminal region and may thus contribute to the substrate binding and/or the specificity of the exonuclease activity.
Similarity Belongs to the arenaviridae nucleocapsid protein family.

Keywords [?]

Gene Ontology [?]

GO:0019029; Cellular component: helical viral capsid.
GO:0030430; Cellular component: host cell cytoplasm.
GO:0019013; Cellular component: viral nucleocapsid.
GO:0046872; Molecular function: metal ion binding.
GO:0003723; Molecular function: RNA binding.
GO:0039689; Biological process: negative stranded viral RNA replication.
GO:0039696; Biological process: RNA-templated viral transcription.
GO:0039724; Biological process: suppression by virus of host viral-induced cytoplasmic pattern recognition receptor signaling pathway via inhibition of IKBKE activity.

Cross-references [?]

Pfam PF17290; Arena_ncap_C; 1;
PF00843; Arena_nucleocap; 1;
PIRSF PIRSF004029; N_ArenaV; 1;

Features [?]

From: NCAP_LASSJ (P13699)
Key     From     To       Description   Tag   Condition   FTGroup
REGION     54     241       Binding site for the cap structure m7GTP        
BINDING     389     389       /ligand="Mn(2+)" /ligand_id="ChEBI:CHEBI:29035     D  
BINDING     391     391       /ligand="Mn(2+)" /ligand_id="ChEBI:CHEBI:29035     E  
BINDING     399     399       /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105     E  
BINDING     506     506       /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105     C  
BINDING     509     509       /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105     H  
BINDING     529     529       /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105     C  
BINDING     533     533       /ligand="Mn(2+)" /ligand_id="ChEBI:CHEBI:29035     D  
SITE     466     466       Important for exonuclease activity     D  

Additional information [?]

Size range 557-570 amino acids
Related rules None
Fusion None