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| Annotation rule MF_04105 |
General rule information
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| Accession | MF_04105 |
| Dates | 18-OCT-2016 (Created) 20-NOV-2019 (Last updated, Version 4) |
| Name | ANTIH_T4 |
| Scope | Viruses; Caudovirales |
| Template | P13304 (ANTIH_BPT4) |
| Triggered by |
Propagated annotation
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Identifier, protein and gene names
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| Identifier |
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| Protein name |
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Comments
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| Similarity | Belongs to the T4likevirus antiholin family. |
Keywords
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case <FTTag:disulf>
end case
Host cell inner membrane
Host cell lysis by virus
Host cell membrane
Host membrane
Membrane
Signal-anchor
Transmembrane
Transmembrane helix
Viral release from host cell
Host cell lysis by virus
Host cell membrane
Host membrane
Membrane
Signal-anchor
Transmembrane
Transmembrane helix
Viral release from host cell
Comments
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| Function | Involved in lysis inhibition. Interacts with and inhibits the holin thereby delaying the host cell lysis timing. Lysis inhibition is imposed when a cell infected by a T4-like virus is superinfected by new incoming viruses. This is probably due to the capsid content of the new phage being released in the periplasm because of blockage of entry due to superinfection exclusion. These periplasmically released viral proteins must somehow activate the periplasmic antiholin, which in turn will block the holin multimerization. |
| Subunit | Interacts (via C-terminus) with holin (via C-terminus); this interaction blocks the holin homomultimerization and delays the host cell lysis. |
| Subcellular location | Host cell inner membrane; Single-pass type II membrane protein. |
| Domain | The transmembrane signal-anchor domain can escape spontaneously from the cell inner membrane. It is called SAR for signal-anchor-release for that reason and explains why the antiholin is an extremly instable protein. Superinfection might stabilize it so that it can inhibit holin multimerization and delay lysis. |
case <FTTag:disulf>
| Ptm | Disulfide bond is required for functionality. |
end case
Gene Ontology
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GO:0020002; Cellular component: host cell plasma membrane.
GO:0016021; Cellular component: integral component of membrane.
GO:1901218; Biological process: negative regulation of holin activity.
GO:0016021; Cellular component: integral component of membrane.
GO:1901218; Biological process: negative regulation of holin activity.
Features
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| From: ANTIH_BPT4 (P13304) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| TRANSMEM | Nter | 24 | Helical; Signal-anchor for type II membrane protein | |||||||||
| TOPO_DOM | 25 | Cter | Periplasmic | |||||||||
| DISULFID | 69 | 75 | disulf | C-x*-C | ||||||||
Additional information
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| Size range | 93-100 amino acids |
| Related rules | None |
| Fusion | None |