HAMAP rule MF_04111

General rule information [?]

Accession	MF_04111
Dates	2-NOV-2016 (Created) 19-NOV-2022 (Last updated, Version 10)

Name	ENDOLYSIN_T7

Scope

Viruses; Caudoviricetes

Template

P00806 (ENLYS_BPT7)

Triggered by

HAMAP; MF_04111 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier

ENLYS

Protein name

RecName:		Full=Endolysin;
		EC 3.5.1.28;
AltName:		Full=N-acetylmuramoyl-L-alanine amidase;

Comments [?]

Function	Plays an important role in the switch between viral transcription and genome replication. Once produced in sufficient amount, interacts with and inhibits the viral RNA polymerase that becomes unable to produce additional late transcripts. This lysozyme-polymerase complex in turn plays an active role in viral genome replication and packaging.
	Endolysin with amidase activity that degrades host peptidoglycans and participates with the holin and spanin proteins in the sequential events which lead to the programmed host cell lysis releasing the mature viral particles. Once the holin has permeabilized the host cell membrane, the endolysin can reach the periplasm and breaking down the peptidoglycan layer.
Catalytic activity	Reaction=Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides.; EC=3.5.1.28;
Cofactor	Zn(2+) Note: Zn(2+) is required for amidase activity
Activity regulation	Binding to the viral RNA polymerase inhibits amidase activity.
Subunit	Interacts with the viral RNA polymerase.
Subcellular location	Host cytoplasm. Note=The endolysin is cytoplasmic, but can reach the periplasmic space with the help of the holins which disrupt the host cell membrane.
Similarity	Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family.

Keywords [?]

Antimicrobial
Bacteriolytic enzyme
Cytolysis
Host cell lysis by virus
Host cytoplasm
Hydrolase
Late protein
Metal-binding
Viral release from host cell
Zinc

Gene Ontology [?]

GO:0008745; Molecular function: N-acetylmuramoyl-L-alanine amidase activity.
GO:0044659; Biological process: viral release from host cell by cytolysis.
GO:0009253; Biological process: peptidoglycan catabolic process.

Cross-references [?]

Pfam	PF01510; Amidase_2; 1;

Features [?]

From: ENLYS_BPT7 (P00806)

Key From To Description Tag Condition FTGroup

BINDING 18 18 /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105 H

BINDING 123 123 /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105 H

BINDING 131 131 /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105 C

SITE 47 47 Essential for amidase activity and zinc hydrate coordination Y

Additional information [?]

Size range	146-153 amino acids
Related rules	None
Fusion	None

View rule in raw text format (no links)