HAMAP rule MF_04111
General rule information
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| Accession | MF_04111 |
| Dates | 2-NOV-2016 (Created)
19-NOV-2022 (Last updated, Version 10) |
| Name | ENDOLYSIN_T7 |
| Scope(s) |
Viruses Caudoviricetes |
| Template(s) | P00806 (ENLYS_BPT7); [ Recover all ] |
| Triggered by |
HAMAP; MF_04111 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | ENLYS |
| Protein name | RecName: Full=Endolysin; EC=3.5.1.28; AltName: Full=N-acetylmuramoyl-L-alanine amidase; |
Comments
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| FUNCTION | Plays an important role in the switch between viral transcription and genome replication. Once produced in sufficient amount, interacts with and inhibits the viral RNA polymerase that becomes unable to produce additional late transcripts. This lysozyme- polymerase complex in turn plays an active role in viral genome replication and packaging. |
| FUNCTION | Endolysin with amidase activity that degrades host peptidoglycans and participates with the holin and spanin proteins in the sequential events which lead to the programmed host cell lysis releasing the mature viral particles. Once the holin has permeabilized the host cell membrane, the endolysin can reach the periplasm and breaking down the peptidoglycan layer. |
| CATALYTIC ACTIVITY | Reaction=Hydrolyzes the link between N-acetylmuramoyl residues and L- amino acid residues in certain cell-wall glycopeptides.; EC=3.5.1.28; |
| COFACTOR | Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Note=Zn(2+) is required for amidase activity |
| ACTIVITY REGULATION | Binding to the viral RNA polymerase inhibits amidase activity. |
| SUBUNIT | Interacts with the viral RNA polymerase. |
| SUBCELLULAR LOCATION | Host cytoplasm. Note=The endolysin is cytoplasmic, but can reach the periplasmic space with the help of the holins which disrupt the host cell membrane. |
| SIMILARITY | Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family. |
Keywords
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| Antimicrobial |
| Bacteriolytic enzyme |
| Cytolysis |
| Host cell lysis by virus |
| Host cytoplasm |
| Hydrolase |
| Late protein |
| Metal-binding |
| Viral release from host cell |
| Zinc |
Gene Ontology
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| GO:0008745; Molecular function:N-acetylmuramoyl-L-alanine amidase activity |
| GO:0044659; Biological process:viral release from host cell by cytolysis |
| GO:0009253; Biological process:peptidoglycan catabolic process |
Cross-references
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| Pfam | PF01510; Amidase_2; 1; |
Features
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| From: ENLYS_BPT7 (P00806) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| BINDING | 18 | 18 | /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" |
H | ||||||||
| BINDING | 123 | 123 | /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" |
H | ||||||||
| BINDING | 131 | 131 | /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" |
C | ||||||||
| SITE | 47 | 47 | /note="Essential for amidase activity and zinc hydrate coordination" | Y | ||||||||
Additional information
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| Size range | 146-153 amino acids |
| Related rules |
None |
| Fusion | Nter: None Cter: None |