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HAMAP rule MF_04111

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General rule information [?]

Accession MF_04111
Dates 2-NOV-2016 (Created)
19-NOV-2022 (Last updated, Version 10)
Viruses; Caudoviricetes
Template P00806 (ENLYS_BPT7)

Propagated annotation [?]

Identifier, protein and gene names [?]

Protein name
RecName: Full=Endolysin;
AltName: Full=N-acetylmuramoyl-L-alanine amidase;

Comments [?]

Function Plays an important role in the switch between viral transcription and genome replication. Once produced in sufficient amount, interacts with and inhibits the viral RNA polymerase that becomes unable to produce additional late transcripts. This lysozyme-polymerase complex in turn plays an active role in viral genome replication and packaging.
Endolysin with amidase activity that degrades host peptidoglycans and participates with the holin and spanin proteins in the sequential events which lead to the programmed host cell lysis releasing the mature viral particles. Once the holin has permeabilized the host cell membrane, the endolysin can reach the periplasm and breaking down the peptidoglycan layer.
Catalytic activity Reaction=Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides.; EC=;
Cofactor Zn(2+)
Note: Zn(2+) is required for amidase activity
Activity regulation Binding to the viral RNA polymerase inhibits amidase activity.
Subunit Interacts with the viral RNA polymerase.
Subcellular location Host cytoplasm. Note=The endolysin is cytoplasmic, but can reach the periplasmic space with the help of the holins which disrupt the host cell membrane.
Similarity Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family.

Keywords [?]

Gene Ontology [?]

GO:0008745; Molecular function: N-acetylmuramoyl-L-alanine amidase activity.
GO:0044659; Biological process: viral release from host cell by cytolysis.
GO:0009253; Biological process: peptidoglycan catabolic process.

Cross-references [?]

Pfam PF01510; Amidase_2; 1;

Features [?]

From: ENLYS_BPT7 (P00806)
Key     From     To       Description   Tag   Condition   FTGroup
BINDING     18     18       /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105     H  
BINDING     123     123       /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105     H  
BINDING     131     131       /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105     C  
SITE     47     47       Essential for amidase activity and zinc hydrate coordination     Y  

Additional information [?]

Size range 146-153 amino acids
Related rules None
Fusion None