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HAMAP rule MF_04111

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General rule information [?]

Accession MF_04111
Dates 2-NOV-2016 (Created)
19-NOV-2022 (Last updated, Version 10)
Scope(s) Viruses
Template(s) P00806 (ENLYS_BPT7); [ Recover all ]
Triggered by HAMAP; MF_04111 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier ENLYS
Protein name RecName: Full=Endolysin;
AltName: Full=N-acetylmuramoyl-L-alanine amidase;

Comments [?]

FUNCTIONPlays an important role in the switch between viral transcription and genome replication. Once produced in sufficient amount, interacts with and inhibits the viral RNA polymerase that becomes unable to produce additional late transcripts. This lysozyme- polymerase complex in turn plays an active role in viral genome replication and packaging.
FUNCTIONEndolysin with amidase activity that degrades host peptidoglycans and participates with the holin and spanin proteins in the sequential events which lead to the programmed host cell lysis releasing the mature viral particles. Once the holin has permeabilized the host cell membrane, the endolysin can reach the periplasm and breaking down the peptidoglycan layer.
CATALYTIC ACTIVITY Reaction=Hydrolyzes the link between N-acetylmuramoyl residues and L- amino acid residues in certain cell-wall glycopeptides.; EC=;
COFACTOR Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Note=Zn(2+) is required for amidase activity
ACTIVITY REGULATIONBinding to the viral RNA polymerase inhibits amidase activity.
SUBUNITInteracts with the viral RNA polymerase.
SUBCELLULAR LOCATIONHost cytoplasm. Note=The endolysin is cytoplasmic, but can reach the periplasmic space with the help of the holins which disrupt the host cell membrane.
SIMILARITYBelongs to the N-acetylmuramoyl-L-alanine amidase 2 family.

Keywords [?]

Gene Ontology [?]

GO:0008745; Molecular function:N-acetylmuramoyl-L-alanine amidase activity
GO:0044659; Biological process:viral release from host cell by cytolysis
GO:0009253; Biological process:peptidoglycan catabolic process

Cross-references [?]

Pfam PF01510; Amidase_2; 1;

Features [?]

From: ENLYS_BPT7 (P00806)
Key From To Description Tag Condition FTGroup
BINDING 18 18 /ligand="Zn(2+)"
BINDING 123 123 /ligand="Zn(2+)"
BINDING 131 131 /ligand="Zn(2+)"
SITE 47 47 /note="Essential for amidase activity and zinc hydrate coordination" Y

Additional information [?]

Size range 146-153 amino acids
Related rules None
Fusion Nter: None Cter: None

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