HAMAP logo

HAMAP rule MF_04121

Send feedback

General rule information [?]

Accession MF_04121
Dates 18-JAN-2018 (Created)
16-FEB-2023 (Last updated, Version 6)
Name TRANSGLYCOSYLASE_T7
Scope
Viruses; Caudoviricetes
Template P03726 (EXLYS_BPT7)
Triggered by

Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
EXLYS
case <FTTag:transglycosylase>
Protein name
RecName: Full=Peptidoglycan transglycosylase gp16;
EC 4.2.2.n1;
AltName: Full=Internal core protein gp16;
else
Protein name
RecName: Full=Putative peptidoglycan transglycosylase gp16;
AltName: Full=Internal core protein gp16;
end case

Comments [?]

case <FTTag:transglycosylase>
Function Component of the cylindrical core that assembles on the inner surface of the capsid during capsid formation and plays a role in viral DNA ejection into the host cell. The inner core is composed of stacked rings of gp14, gp15 and gp16 proteins. Following binding to the host cell surface, the internal core is disassembled and gp16 is ejected along with gp14 and gp15 into the infected cell. Gp16 probably inserts in the host inner membrane and remains associated with gp15. The gp15-gp16 complex binds to both the viral DNA and the host inner membrane, probably escorting the leading end of the genome through the periplasm and controlling the extent of DNA translocated into the host cell. Functions as an exolysin that catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N-acetylglucosamine residues in peptidoglycans allowing the local digestion of the bacterial peptidoglycan wall.
else
Function Component of the cylindrical core that assembles on the inner surface of the capsid during capsid formation and plays a role in viral DNA ejection into the host cell. The inner core is composed of stacked rings of gp14, gp15 and gp16 proteins. Following binding to the host cell surface, the internal core is disassembled and gp16 is ejected along with gp14 and gp15 into the infected cell. Gp16 probably inserts in the host inner membrane and remains associated with gp15. The gp15-gp16 complex binds to both the viral DNA and the host inner membrane, probably escorting the leading end of the genome through the periplasm and controlling the extent of DNA translocated into the host cell.
end case
case <FTTag:transglycosylase>
Catalytic activity Reaction=Exolytic cleavage of the (1->4)-beta-glycosidic linkage between N-acetylmuramic acid (MurNAc) and N-acetylglucosamine (GlcNAc) residues in peptidoglycan, from either the reducing or the non-reducing ends of the peptidoglycan chains, with concomitant formation of a 1,6-anhydrobond in the MurNAc residue.; EC=4.2.2.n1;
end case
Subunit Homotetramer. Interacts with gp15; after ejection the gp15-gp16 complex composed of a gp15 octamer and a gp16 tetramer probably binds both the viral DNA and the host inner membrane.
Subcellular location Virion. Host cell inner membrane; Single-pass membrane protein. Note=The gp15-gp16 complex spans the periplasm and the cytoplasmic membrane.
case <FTTag:transglycosylase>
Domain The N-terminus contains the transglycosylase activity. The C-terminus is essential for the viral DNA translocation into the host cytoplasm.
end case
Similarity Belongs to the transglycosylase Slt family.

Keywords [?]

Host cell inner membrane
Host cell membrane
Host membrane
case <FTTag:transglycosylase>
Antimicrobial
Bacteriolytic enzyme
Degradation of host cell envelope components during virus entry
Degradation of host peptidoglycans during virus entry
Hydrolase
Lyase
end case
Membrane
Transmembrane
Transmembrane helix
Viral genome ejection through host cell envelope
Viral penetration into host cytoplasm
Viral short tail ejection system
Virion
Virus entry into host cell

Gene Ontology [?]

GO:0044423; Cellular component: virion component.
GO:0098932; Biological process: disruption by virus of host cell wall peptidoglycan during virus entry.
GO:0099002; Biological process: viral genome ejection through host cell envelope, short tail mechanism.
case <FTTag:transglycosylase>
GO:0008933; Molecular function: lytic transglycosylase activity.
end case

Cross-references [?]

PROSITE PS00922; TRANSGLYCOSYLASE; 1;
Pfam PF01464; SLT; 1;

Computed features [?]

General Coiled_coil; -; 0-unlimited; trigger=yes;
Transmembrane; -; 0-1;

Features [?]

From: EXLYS_BPT7 (P03726)
Key     From     To       Description   Tag   Condition   FTGroup
TOPO_DOM     Nter     1136       Periplasmic        
TRANSMEM     1137     1157       Helical        
TOPO_DOM     1158     Cter       Cytoplasmic        
REGION     24     111       Transglycosylase SLT-type domain        
REGION     1314     1318       Essential for viral DNA translocation     R-E-[KR]-[KR]-K  
ACT_SITE     37     37           transglycosylase   E  

Additional information [?]

Size range 1250-1550 amino acids
Related rules None
Fusion None


View rule in raw text format (no links)