HAMAP rule MF_04121
General rule information
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| Accession | MF_04121 |
| Dates | 18-JAN-2018 (Created)
16-FEB-2023 (Last updated, Version 6) |
| Name | TRANSGLYCOSYLASE_T7 |
| Scope(s) |
Viruses Caudoviricetes |
| Template(s) | P03726 (EXLYS_BPT7); [ Recover all ] |
| Triggered by |
HAMAP; MF_04121 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | EXLYS |
| case <FTTag:transglycosylase> | |
| Protein name | RecName: Full=Peptidoglycan transglycosylase gp16; EC=4.2.2.n1; AltName: Full=Internal core protein gp16; |
| else | |
| Protein name | RecName: Full=Putative peptidoglycan transglycosylase gp16; AltName: Full=Internal core protein gp16; |
| end case | |
Comments
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| case <FTTag:transglycosylase> | |
| FUNCTION | Component of the cylindrical core that assembles on the inner surface of the capsid during capsid formation and plays a role in viral DNA ejection into the host cell. The inner core is composed of stacked rings of gp14, gp15 and gp16 proteins. Following binding to the host cell surface, the internal core is disassembled and gp16 is ejected along with gp14 and gp15 into the infected cell. Gp16 probably inserts in the host inner membrane and remains associated with gp15. The gp15- gp16 complex binds to both the viral DNA and the host inner membrane, probably escorting the leading end of the genome through the periplasm and controlling the extent of DNA translocated into the host cell. Functions as an exolysin that catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N-acetylglucosamine residues in peptidoglycans allowing the local digestion of the bacterial peptidoglycan wall. |
| else | |
| FUNCTION | Component of the cylindrical core that assembles on the inner surface of the capsid during capsid formation and plays a role in viral DNA ejection into the host cell. The inner core is composed of stacked rings of gp14, gp15 and gp16 proteins. Following binding to the host cell surface, the internal core is disassembled and gp16 is ejected along with gp14 and gp15 into the infected cell. Gp16 probably inserts in the host inner membrane and remains associated with gp15. The gp15- gp16 complex binds to both the viral DNA and the host inner membrane, probably escorting the leading end of the genome through the periplasm and controlling the extent of DNA translocated into the host cell. |
| end case | |
| case <FTTag:transglycosylase> | |
| CATALYTIC ACTIVITY | Reaction=Exolytic cleavage of the (1->4)-beta-glycosidic linkage between N-acetylmuramic acid (MurNAc) and N-acetylglucosamine (GlcNAc) residues in peptidoglycan, from either the reducing or the non-reducing ends of the peptidoglycan chains, with concomitant formation of a 1,6-anhydrobond in the MurNAc residue.; EC=4.2.2.n1; |
| end case | |
| SUBUNIT | Homotetramer. Interacts with gp15; after ejection the gp15- gp16 complex composed of a gp15 octamer and a gp16 tetramer probably binds both the viral DNA and the host inner membrane. |
| SUBCELLULAR LOCATION | Virion. Host cell inner membrane; Single-pass membrane protein. Note=The gp15-gp16 complex spans the periplasm and the cytoplasmic membrane. |
| case <FTTag:transglycosylase> | |
| DOMAIN | The N-terminus contains the transglycosylase activity. The C- terminus is essential for the viral DNA translocation into the host cytoplasm. |
| end case | |
| SIMILARITY | Belongs to the transglycosylase Slt family. |
Keywords
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Gene Ontology
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| GO:0044423; Cellular component:virion component | |
| GO:0098932; Biological process:disruption by virus of host cell wall peptidoglycan during virus entry | |
| GO:0099002; Biological process:viral genome ejection through host cell envelope, short tail mechanism | |
| case <FTTag:transglycosylase> | |
| GO:0008933; Molecular function:lytic transglycosylase activity | |
| end case | |
Cross-references
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| PROSITE | PS00922; TRANSGLYCOSYLASE; 1; |
| Pfam | PF01464; SLT; 1; |
| General | Coiled_coil; -; 0-unlimited; |
| General | Transmembrane; -; 0-1; |
Features
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| From: EXLYS_BPT7 (P03726) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| TOPO_DOM | Nter | 1136 | /note="Periplasmic" | |||||||||
| TRANSMEM | 1137 | 1157 | /note="Helical" | |||||||||
| TOPO_DOM | 1158 | Cter | /note="Cytoplasmic" | |||||||||
| REGION | 24 | 111 | /note="Transglycosylase SLT-type domain" | |||||||||
| REGION | 1314 | 1318 | /note="Essential for viral DNA translocation" | R-E-[KR]-[KR]-K | ||||||||
| ACT_SITE | 37 | 37 | transglycosylase | E | ||||||||
Additional information
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| Size range | 1250-1550 amino acids |
| Related rules |
None |
| Fusion | Nter: None Cter: None |