HAMAP logo

HAMAP rule MF_04137

Send feedback

General rule information [?]

Accession MF_04137
Dates 16-APR-2019 (Created)
29-SEP-2022 (Last updated, Version 9)
Template P27358 (SPAN1_BPP21)

Propagated annotation [?]

Identifier, protein and gene names [?]

Protein name
RecName: Full=Spanin, inner membrane subunit;
AltName: Full=Lysis protein Rz;

Comments [?]

Function Component of the spanin complex that disrupts the host outer membrane and participates in cell lysis during virus exit. The spanin complex conducts the final step in host lysis by disrupting the outer membrane after holin and endolysin action have permeabilized the inner membrane and degraded the host peptidoglycans. Host outer membrane disruption is due to local fusion between the inner and outer membrane performed by the spanin complex.
case <FTGroup:1>
Subunit Homodimer; disulfide-linked. Interacts (via C-terminus) with the spanin outer lipoprotein subunit (via C-terminus). Part of the spanin complex which spans the entire periplasmic space. The spanin complex is composed of one homodimer of the i-spanin linked by intermolecular disulfide bonds involving two Cys residues and one homodimer of the o-spanin covalently linked by an intermolecular disulfide bond involving one Cys.
end case
case <AnyFeature:Transmembrane<2>
Subcellular location Host cell inner membrane; Single-pass type II membrane protein; Periplasmic side.
end case
case <Feature:Coiled_coil>
Domain The coiled coil region is probably involved in host membrane fusion leading to lysis.
end case
Similarity Belongs to the Lambdavirus i-spanin family.

Keywords [?]

case <Feature:coiled>
end case
case <FTGroup:1>
end case
case <Feature:Transmembrane>
end case

Gene Ontology [?]

GO:0020002; Cellular component: host cell plasma membrane.
GO:0016020; Cellular component: membrane.
GO:0044659; Biological process: viral release from host cell by cytolysis.

Cross-references [?]

Pfam PF03245; Phage_lysis; 1;

Computed features [?]

General Coiled_coil; -; 0-unlimited; trigger=yes;
case <Feature:Transmembrane==1>
ADD_TOPO_DOMAIN Cytoplasmic; -; 1; trigger=yes;
Periplasmic; -; 1; trigger=yes;
General Transmembrane; -; 0-1; trigger=yes;
end case

Features [?]

From: SPAN1_LAMBD (P00726)
Key     From     To       Description   Tag   Condition   FTGroup
DISULFID     99     99       Interchain     C   1
DISULFID     152     152       Interchain     C   1
case not <Feature:Transmembrane==1>
TOPO_DOM     Nter     3       Cytoplasmic        
TRANSMEM     4     24       Helical; Signal-anchor for type II membrane protein        
TOPO_DOM     25     Cter       Periplasmic        
end case

Additional information [?]

Size range 95-195 amino acids
Related rules None
Fusion None