HAMAP logo

HAMAP rule MF_04143

Send feedback

General rule information [?]

Accession MF_04143
Dates 12-DEC-2019 (Created)
1-MAR-2024 (Last updated, Version 7)
Name Poxins
Scope(s) Eukaryota
Template(s) Q01225 (POXIN_VACCW); [ Recover all ]
Triggered by HAMAP; MF_04143 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier POXIN
case <Feature:PF17057>
Protein name RecName: Full=Poxin-Schlafen;
Protein name RecName: Full=Poxin;
end case

Comments [?]

case <OC:Poxviridae>
FUNCTIONNuclease that is responsible for viral evasion of host cGAS- STING innate immunity. Cleaves 2',3'-cGAMP which is produced by host cGAS following recognition of cytosolic DNA and blocks the subsequent 2',3'-cGAMP-mediated activation of TMEM173/STING, which normally spreads to adjacent cells and activates the interferon and NF-kappa-B immune responses.
else case <OC:Viruses>
FUNCTIONNuclease that cleaves host 2',3'-cGAMP.
end case
case <OC:Insecta>
FUNCTIONNuclease that cleaves 2',3'-cGAMP.
end case
CATALYTIC ACTIVITY Reaction=2',3'-cGAMP + H2O = Gp(2'-5')Ap(3') + H(+); Xref=Rhea:RHEA:59472, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:143093, ChEBI:CHEBI:143098; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:59473;
case <OC:Viruses>
DOMAINThe substrate binding site is formed by the N-terminus of a monomer and the C-terminus of the opposite monomer.
end case
case <Feature:PF17057>
SIMILARITYIn the N-terminal section; belongs to the poxin family.
SIMILARITYIn the C-terminal section; belongs to the Schlafen protein family. Subgroup poxviridae B3 subfamily.
else case <OC:Viruses>
SIMILARITYBelongs to the poxin family.
else case <OC:Insecta>
SIMILARITYBelongs to the poxin family. Highly divergent.
CAUTIONThe active site cannot be determined by similarity to the viral poxins.
end case

Keywords [?]

Gene Ontology [?]

GO:0061507; Molecular function:2',3'-cyclic GMP-AMP binding
GO:0004518; Molecular function:nuclease activity
case <OCellular component:Poxviridae>
GO:0052170; Biological process:symbiont-mediated suppression of host innate immune response
end case

Cross-references [?]

Pfam PF04766; Baculo_p26; 1;
Pfam PF04326; AlbA_2; 0-1;
Pfam PF17057; B3R; 0-1;

Features [?]

From: POXIN_VACCW (Q01225)
Key From To Description Tag Condition FTGroup
case <OC:Viruses>
ACT_SITE 17 17 /note="Proton donor" H
ACT_SITE 138 138 /note="Shared with catalytic histidine of dimeric partner" Y
ACT_SITE 142 142 /note="Proton acceptor; shared with catalytic histidine of dimeric partner" K
SITE 60 60 /note="Substrate binding" R
SITE 105 105 /note="Substrate binding" I
SITE 149 149 /note="Substrate binding" N
SITE 169 169 /note="Substrate binding" Q
SITE 182 182 /note="Substrate binding" R
SITE 184 184 /note="Substrate binding" R
SITE 186 186 /note="Substrate binding" K
end case
Key From To Description Tag Condition FTGroup
case <Feature:PF17057>
REGION Nter 238 /note="Poxin-like"
REGION 239 Cter /note="Schlafen-like"
end case

Additional information [?]

Size range 180-550 amino acids
Related rules None
Fusion Nter: None Cter: None

View rule in raw text format (no links)