HAMAP rule MF_04143
General rule information
[?]
| Accession | MF_04143 |
| Dates | 12-DEC-2019 (Created) 28-OCT-2021 (Last updated, Version 6) |
| Name | Poxins |
| Scope | Eukaryota
Viruses |
| Template | Q01225 (POXIN_VACCW) |
| Triggered by |
Propagated annotation
[?]
Identifier, protein and gene names
[?]
| Identifier |
|
case <Feature:PF17057>
| Protein name |
|
||||||||
else
| Protein name |
|
||||||||
end case
Comments
[?]
case <OC:Poxviridae>
| Function | Nuclease that is responsible for viral evasion of host cGAS-STING innate immunity. Cleaves 2',3'-cGAMP which is produced by host cGAS following recognition of cytosolic DNA and blocks the subsequent 2',3'-cGAMP-mediated activation of TMEM173/STING, which normally spreads to adjacent cells and activates the interferon and NF-kappa-B immune responses. |
else case <OC:Viruses>
| Function | Nuclease that cleaves host 2',3'-cGAMP. |
end case
case <OC:Insecta>
| Function | Nuclease that cleaves 2',3'-cGAMP. |
end case
| Catalytic activity | RHEA:59472: 2',3'-cGAMP + H2O = Gp(2'-5')Ap(3') + H(+)
PhysiologicalDirection=left-to-right (RHEA:59473) |
case <OC:Viruses>
| Subunit | Homodimer. |
| Domain | The substrate binding site is formed by the N-terminus of a monomer and the C-terminus of the opposite monomer. |
end case
case <Feature:PF17057>
| Similarity | In the N-terminal section; belongs to the poxin family. |
| In the C-terminal section; belongs to the Schlafen protein family. Subgroup poxviridae B3 subfamily. |
else case <OC:Viruses>
| Similarity | Belongs to the poxin family. |
else case <OC:Insecta>
| Similarity | Belongs to the poxin family. Highly divergent. |
| Caution | The active site cannot be determined by similarity to the viral poxins. |
end case
Keywords
[?]
Gene Ontology
[?]
GO:0061507; Molecular function: 2',3'-cyclic GMP-AMP binding.
GO:0004518; Molecular function: nuclease activity.
GO:0004518; Molecular function: nuclease activity.
case <OC:Poxviridae>
GO:0039503; Biological process: suppression by virus of host innate immune response.
end case
Cross-references
[?]
Features
[?]
case <OC:Viruses>
| From: POXIN_VACCW (Q01225) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| ACT_SITE | 17 | 17 | Proton donor | H | ||||||||
| ACT_SITE | 138 | 138 | Shared with catalytic histidine of dimeric partner | Y | ||||||||
| ACT_SITE | 142 | 142 | Proton acceptor; shared with catalytic histidine of dimeric partner | K | ||||||||
| SITE | 60 | 60 | Substrate binding | R | ||||||||
| SITE | 105 | 105 | Substrate binding | I | ||||||||
| SITE | 149 | 149 | Substrate binding | N | ||||||||
| SITE | 169 | 169 | Substrate binding | Q | ||||||||
| SITE | 182 | 182 | Substrate binding | R | ||||||||
| SITE | 184 | 184 | Substrate binding | R | ||||||||
| SITE | 186 | 186 | Substrate binding | K | ||||||||
end case
case <Feature:PF17057>
| From: POXIN_CWPXB (Q8QMP8) | ||||||||||||
| REGION | Nter | 238 | Poxin-like | |||||||||
| REGION | 239 | Cter | Schlafen-like | |||||||||
end case
Additional information
[?]
| Size range | 180-550 amino acids |
| Related rules | None |
| Fusion | None |