HAMAP rule MF_04143
General rule information
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| Accession | MF_04143 |
| Dates | 12-DEC-2019 (Created)
1-MAR-2024 (Last updated, Version 7) |
| Name | Poxins |
| Scope(s) |
Eukaryota Viruses |
| Template(s) | Q01225 (POXIN_VACCW); [ Recover all ] |
| Triggered by |
HAMAP; MF_04143 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | POXIN |
| case <Feature:PF17057> | |
| Protein name | RecName: Full=Poxin-Schlafen; EC=3.1.-.-; |
| else | |
| Protein name | RecName: Full=Poxin; EC=3.1.-.-; |
| end case | |
Comments
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| case <OC:Poxviridae> | |
| FUNCTION | Nuclease that is responsible for viral evasion of host cGAS- STING innate immunity. Cleaves 2',3'-cGAMP which is produced by host cGAS following recognition of cytosolic DNA and blocks the subsequent 2',3'-cGAMP-mediated activation of TMEM173/STING, which normally spreads to adjacent cells and activates the interferon and NF-kappa-B immune responses. |
| else case <OC:Viruses> | |
| FUNCTION | Nuclease that cleaves host 2',3'-cGAMP. |
| end case | |
| case <OC:Insecta> | |
| FUNCTION | Nuclease that cleaves 2',3'-cGAMP. |
| end case | |
| CATALYTIC ACTIVITY | Reaction=2',3'-cGAMP + H2O = Gp(2'-5')Ap(3') + H(+); Xref=Rhea:RHEA:59472, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:143093, ChEBI:CHEBI:143098; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:59473; |
| case <OC:Viruses> | |
| SUBUNIT | Homodimer. |
| DOMAIN | The substrate binding site is formed by the N-terminus of a monomer and the C-terminus of the opposite monomer. |
| end case | |
| case <Feature:PF17057> | |
| SIMILARITY | In the N-terminal section; belongs to the poxin family. |
| SIMILARITY | In the C-terminal section; belongs to the Schlafen protein family. Subgroup poxviridae B3 subfamily. |
| else case <OC:Viruses> | |
| SIMILARITY | Belongs to the poxin family. |
| else case <OC:Insecta> | |
| SIMILARITY | Belongs to the poxin family. Highly divergent. |
| CAUTION | The active site cannot be determined by similarity to the viral poxins. |
| end case | |
Keywords
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Gene Ontology
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| GO:0061507; Molecular function:2',3'-cyclic GMP-AMP binding | |
| GO:0004518; Molecular function:nuclease activity | |
| case <OCellular component:Poxviridae> | |
| GO:0052170; Biological process:symbiont-mediated suppression of host innate immune response | |
| end case | |
Cross-references
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Features
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| From: POXIN_VACCW (Q01225) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| case <OC:Viruses> | ||||||||||||
| ACT_SITE | 17 | 17 | /note="Proton donor" | H | ||||||||
| ACT_SITE | 138 | 138 | /note="Shared with catalytic histidine of dimeric partner" | Y | ||||||||
| ACT_SITE | 142 | 142 | /note="Proton acceptor; shared with catalytic histidine of dimeric partner" | K | ||||||||
| SITE | 60 | 60 | /note="Substrate binding" | R | ||||||||
| SITE | 105 | 105 | /note="Substrate binding" | I | ||||||||
| SITE | 149 | 149 | /note="Substrate binding" | N | ||||||||
| SITE | 169 | 169 | /note="Substrate binding" | Q | ||||||||
| SITE | 182 | 182 | /note="Substrate binding" | R | ||||||||
| SITE | 184 | 184 | /note="Substrate binding" | R | ||||||||
| SITE | 186 | 186 | /note="Substrate binding" | K | ||||||||
| end case | ||||||||||||
| From: POXIN_CWPXB (Q8QMP8) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| case <Feature:PF17057> | ||||||||||||
| REGION | Nter | 238 | /note="Poxin-like" | |||||||||
| REGION | 239 | Cter | /note="Schlafen-like" | |||||||||
| end case | ||||||||||||
Additional information
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| Size range | 180-550 amino acids |
| Related rules |
None |
| Fusion | Nter: None Cter: None |