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HAMAP rule MF_04143

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General rule information [?]

Accession MF_04143
Dates 12-DEC-2019 (Created)
28-OCT-2021 (Last updated, Version 6)
Name Poxins
Template Q01225 (POXIN_VACCW)

Propagated annotation [?]

Identifier, protein and gene names [?]

case <Feature:PF17057>
Protein name
RecName: Full=Poxin-Schlafen;
EC 3.1.-.-;
Protein name
RecName: Full=Poxin;
EC 3.1.-.-;
end case

Comments [?]

case <OC:Poxviridae>
Function Nuclease that is responsible for viral evasion of host cGAS-STING innate immunity. Cleaves 2',3'-cGAMP which is produced by host cGAS following recognition of cytosolic DNA and blocks the subsequent 2',3'-cGAMP-mediated activation of TMEM173/STING, which normally spreads to adjacent cells and activates the interferon and NF-kappa-B immune responses.
else case <OC:Viruses>
Function Nuclease that cleaves host 2',3'-cGAMP.
end case
case <OC:Insecta>
Function Nuclease that cleaves 2',3'-cGAMP.
end case
Catalytic activity RHEA:59472: 2',3'-cGAMP + H2O = Gp(2'-5')Ap(3') + H(+)
PhysiologicalDirection=left-to-right (RHEA:59473)
case <OC:Viruses>
Subunit Homodimer.
Domain The substrate binding site is formed by the N-terminus of a monomer and the C-terminus of the opposite monomer.
end case
case <Feature:PF17057>
Similarity In the N-terminal section; belongs to the poxin family.
In the C-terminal section; belongs to the Schlafen protein family. Subgroup poxviridae B3 subfamily.
else case <OC:Viruses>
Similarity Belongs to the poxin family.
else case <OC:Insecta>
Similarity Belongs to the poxin family. Highly divergent.
Caution The active site cannot be determined by similarity to the viral poxins.
end case

Keywords [?]

Gene Ontology [?]

GO:0061507; Molecular function: 2',3'-cyclic GMP-AMP binding.
GO:0004518; Molecular function: nuclease activity.
case <OC:Poxviridae>
GO:0039503; Biological process: suppression by virus of host innate immune response.
end case

Cross-references [?]

Pfam PF04766; Baculo_p26; 1;
PF04326; AlbA_2; 0-1;
PF17057; B3R; 0-1;

Features [?]

case <OC:Viruses>
From: POXIN_VACCW (Q01225)
Key     From     To       Description   Tag   Condition   FTGroup
ACT_SITE     17     17       Proton donor     H  
ACT_SITE     138     138       Shared with catalytic histidine of dimeric partner     Y  
ACT_SITE     142     142       Proton acceptor; shared with catalytic histidine of dimeric partner     K  
SITE     60     60       Substrate binding     R  
SITE     105     105       Substrate binding     I  
SITE     149     149       Substrate binding     N  
SITE     169     169       Substrate binding     Q  
SITE     182     182       Substrate binding     R  
SITE     184     184       Substrate binding     R  
SITE     186     186       Substrate binding     K  
end case
case <Feature:PF17057>
REGION     Nter     238       Poxin-like        
REGION     239     Cter       Schlafen-like        
end case

Additional information [?]

Size range 180-550 amino acids
Related rules None
Fusion None