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HAMAP rule MF_04151

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General rule information [?]

Accession MF_04151
Dates 15-JAN-2021 (Created)
25-MAR-2021 (Last updated, Version 3)
Name NEEDLE_T4
Scope(s) Viruses
Template(s) P16009 (NEEDL_BPT4); [ Recover all ]
Triggered by HAMAP; MF_04151 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier NEEDL
case <FTTag:Cleavage> and <OC:Tequatrovirus>
Protein name RecName: Full=Pre-baseplate central spike protein Gp5;
                 Short=Pre-Gp5;
AltName: Full=Peptidoglycan hydrolase gp5;
                 EC=3.2.1.17;
                 Contains:
RecName: Full=Baseplate central spike protein Gp5*;
AltName: Full=Mature Gp5;
                 Contains:
RecName: Full=Gp5C;
Gene name Name=5;
else case <FTTag:Cleavage> and not <OC:Tequatrovirus>
Protein name RecName: Full=Pre-baseplate central spike protein;
AltName: Full=Peptidoglycan hydrolase;
                 EC=3.2.1.17;
                 Contains:
RecName: Full=Mature baseplate central spike protein;
                 Contains:
RecName: Full=Baseplate central spike protein C-terminus;
else
Protein name RecName: Full=Baseplate central spike protein;
AltName: Full=Peptidoglycan hydrolase;
                 EC=3.2.1.17;
end case

Comments [?]

case <FTTag:Cleavage> and <OC:Tequatrovirus>
FUNCTION[Baseplate central spike protein Gp5*]: Baseplate central spike complex-associated lysozyme that is essential for the localized hydrolysis of bacterial cell wall, so that the tail tube, through which the phage DNA is ejected, can penetrate to the host inner membrane. The tail lysozyme complex at the tip of the tail tube penetrates through the outer membrane into the periplasm and during that process, gp5* dissociates from gp5C and activated. Due to the lower pH in the periplasm, gp5* would dissociate from gp27 which probably still binds to the tip of the tube. This way, lysozyme domain is released and locally digests the peptidoglycan layer to make a hole to let the tube penetrate to the inner membrane. Involved in the tail assembly.
CATALYTIC ACTIVITY[Baseplate central spike protein Gp5*]: Reaction=Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins.; EC=3.2.1.17;
SUBUNIT[Baseplate central spike protein Gp5*]: Monomer. The central spike complex, which creates an extension of the tail tube, is made up of three copies of the gp27-gp5*-gp5C complex and one copy of gp5.4. Part of the baseplate macromolecular complex which consists of gp5*, gp5C, gp5.4, gp27 (central spike complex); gp6, gp25, gp53 (inner baseplate); gp7, gp8 (intermediate baseplate); gp9, gp10, gp11, gp12 (peripheral); gp48 and gp54 (proximal region of the tail tube).
SUBUNIT[Gp5C]: Homotrimer. The central spike complex, which creates an extension of the tail tube, is made up of three copies of the gp27- gp5*-gp5C complex and one copy of gp5.4. Part of the baseplate macromolecular complex which consists of gp5*, gp5C, gp5.4, gp27 (central spike complex); gp6, gp25, gp53 (inner baseplate); gp7, gp8 (intermediate baseplate); gp9, gp10, gp11, gp12 (peripheral); gp48 and gp54 (proximal region of the tail tube).
SUBUNIT[Pre-baseplate central spike protein Gp5]: Homotrimer.
SUBCELLULAR LOCATION[Baseplate central spike protein Gp5*]: Virion. Note=Present in 3 copies in the baseplate.
SUBCELLULAR LOCATION[Gp5C]: Virion. Note=Present in 3 copies in the baseplate.
SUBCELLULAR LOCATION[Pre-baseplate central spike protein Gp5]: Virion. Note=Present in the baseplate.
PTM[Pre-baseplate central spike protein Gp5]: In the fully assembled virus, gp5 precursor is cleaved to form the mature tail lysozyme gp5*, and a C-terminus fragment, gp5C. The two fragments remain associated with the virion. The enzymatic activity of the precursor is about 10% of that of mature gp5*.
SIMILARITYBelongs to the glycosyl hydrolase 24 family.
else case <FTTag:Cleavage> and not <OC:Tequatrovirus>
FUNCTION[Mature baseplate central spike protein]: Baseplate central spike complex-associated lysozyme that is essential for the localized hydrolysis of bacterial cell wall, so that the tail tube, through which the phage DNA is ejected, can penetrate to the host inner membrane. The tail lysozyme complex at the tip of the tail tube penetrates through the outer membrane into the periplasm. This way, lysozyme domain is released and locally digests the peptidoglycan layer to make a hole to let the tube penetrate to the inner membrane. Involved in the tail assembly.
CATALYTIC ACTIVITY[Mature baseplate central spike protein]: Reaction=Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins.; EC=3.2.1.17;
SUBUNIT[Mature baseplate central spike protein]: Monomer. The central spike complex creates an extension of the tail tube.
SUBUNIT[Baseplate central spike protein C-terminus]: Homotrimer. The central spike complex creates an extension of the tail tube.
SUBUNIT[Pre-baseplate central spike protein]: Homotrimer.
SUBCELLULAR LOCATION[Mature baseplate central spike protein]: Virion. Note=Present in the baseplate.
SUBCELLULAR LOCATION[Baseplate central spike protein C-terminus]: Virion. Note=Present in the baseplate.
SUBCELLULAR LOCATION[Pre-baseplate central spike protein]: Virion. Note=Present in the baseplate.
PTM[Pre-baseplate central spike protein]: In the fully assembled virus, the pre-baseplate central spike protein is cleaved to form the mature baseplate central spike protein, and a C-terminus fragment, the baseplate central spike protein C-terminus. The two fragments remain associated with the virion.
CAUTIONThe cleavage site between the mature baseplate central spike protein and the baseplate central spike protein C-terminus is uncertain.
SIMILARITYBelongs to the glycosyl hydrolase 24 family.
else
FUNCTION[Baseplate central spike protein]: Baseplate central spike complex-associated lysozyme that is essential for the localized hydrolysis of bacterial cell wall, so that the tail tube, through which the phage DNA is ejected, can penetrate to the host inner membrane. The tail lysozyme complex at the tip of the tail tube penetrates through the outer membrane into the periplasm. This way, lysozyme domain is released and locally digests the peptidoglycan layer to make a hole to let the tube penetrate to the inner membrane. Involved in the tail assembly.
CATALYTIC ACTIVITY[Baseplate central spike protein]: Reaction=Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins.; EC=3.2.1.17;
SIMILARITYBelongs to the glycosyl hydrolase 24 family.
end case

Keywords [?]


Gene Ontology [?]

GO:0098015; Cellular component:virus tail
GO:0098025; Cellular component:virus tail, baseplate
GO:0003796; Molecular function:lysozyme activity
GO:0098932; Biological process:disruption by virus of host cell wall peptidoglycan during virus entry
GO:0009253; Biological process:peptidoglycan catabolic process
GO:0098003; Biological process:viral tail assembly

Cross-references [?]

Pfam PF06715; Gp5_C; 3;
Pfam PF06714; Gp5_OB; 1;
Pfam PF00959; Phage_lysozyme; 1;
PRINTS PR00684; T4LYSOZYME; 1;

Features [?]

From: NEEDL_BPT4 (P16009)
Key From To Description Tag Condition FTGroup
ACT_SITE 184 184 /note="Proton donor" E
ACT_SITE 193 193 /note="Nucleophile" D
SITE 351 352 /note="Cleavage" Cleavage S-A
case <FTTag:Cleavage> and <OC:Tequatrovirus>
CHAIN Nter Cter /note="Pre-baseplate central spike protein Gp5"
CHAIN Nter 351 /note="Baseplate central spike protein Gp5*"
CHAIN 352 Cter /note="Gp5C"
else case <FTTag:Cleavage> and not <OC:Tequatrovirus>
CHAIN Nter Cter /note="Pre-baseplate central spike protein"
CHAIN Nter 351 /note="Mature baseplate central spike protein"
CHAIN 352 Cter /note="Baseplate central spike protein C-terminus"
else
CHAIN Nter Cter /note="Baseplate central spike protein"
end case

Additional information [?]

Size range 550-700 amino acids
Related rules None
Fusion Nter: None Cter: None



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