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Annotation rule MF_04151
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General rule information [?]

Accession MF_04151
Dates 15-JAN-2021 (Created)
15-JAN-2021 (Last updated, Version 2)
Name NEEDLE_T4
Scope
Viruses
Template P16009 (NEEDL_BPT4)

Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
NEEDL
case <FTTag:Cleavage> and <OC:Tequatrovirus>
Protein name
RecName: Full=Pre-baseplate central spike protein Gp5;
Short=Pre-Gp5;
AltName: Full=Peptidoglycan hydrolase gp5;
EC 3.2.1.17;
Contains:
RecName: Full=Baseplate central spike protein Gp5*;
AltName: Full=Mature Gp5;
Contains:
RecName: Full=Gp5C;
Gene name
5
else case <FTTag:Cleavage> and not <OC:Tequatrovirus>
Protein name
RecName: Full=Pre-baseplate central spike protein;
AltName: Full=Peptidoglycan hydrolase;
EC 3.2.1.17;
Contains:
RecName: Full=Mature baseplate central spike protein;
Contains:
RecName: Full=Baseplate central spike protein C-terminus;
else
Protein name
RecName: Full=Baseplate central spike protein;
AltName: Full=Peptidoglycan hydrolase;
EC 3.2.1.17;
end case

Comments [?]

case <FTTag:Cleavage> and <OC:Tequatrovirus>
Function [Baseplate central spike protein Gp5*]: Baseplate central spike complex-associated lysozyme that is essential for the localized hydrolysis of bacterial cell wall, so that the tail tube, through which the phage DNA is ejected, can penetrate to the host inner membrane. The tail lysozyme complex at the tip of the tail tube penetrates through the outer membrane into the periplasm and during that process, gp5* dissociates from gp5C and activated. Due to the lower pH in the periplasm, gp5* would dissociate from gp27 which probably still binds to the tip of the tube. This way, lysozyme domain is released and locally digests the peptidoglycan layer to make a hole to let the tube penetrate to the inner membrane. Involved in the tail assembly.
Catalytic activity [Baseplate central spike protein Gp5*]: Reaction=Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins.; EC=3.2.1.17;
Subunit [Baseplate central spike protein Gp5*]: Monomer. The central spike complex, which creates an extension of the tail tube, is made up of three copies of the gp27-gp5*-gp5C complex and one copy of gp5.4. Part of the baseplate macromolecular complex which consists of gp5*, gp5C, gp5.4, gp27 (central spike complex); gp6, gp25, gp53 (inner baseplate); gp7, gp8 (intermediate baseplate); gp9, gp10, gp11, gp12 (peripheral); gp48 and gp54 (proximal region of the tail tube).
[Gp5C]: Homotrimer. The central spike complex, which creates an extension of the tail tube, is made up of three copies of the gp27-gp5*-gp5C complex and one copy of gp5.4. Part of the baseplate macromolecular complex which consists of gp5*, gp5C, gp5.4, gp27 (central spike complex); gp6, gp25, gp53 (inner baseplate); gp7, gp8 (intermediate baseplate); gp9, gp10, gp11, gp12 (peripheral); gp48 and gp54 (proximal region of the tail tube).
[Pre-baseplate central spike protein Gp5]: Homotrimer.
Subcellular location [Baseplate central spike protein Gp5*]: Virion. Note=Present in 3 copies in the baseplate.
[Gp5C]: Virion. Note=Present in 3 copies in the baseplate.
[Pre-baseplate central spike protein Gp5]: Virion. Note=Present in the baseplate.
Ptm [Pre-baseplate central spike protein Gp5]: In the fully assembled virus, gp5 precursor is cleaved to form the mature tail lysozyme gp5*, and a C-terminus fragment, gp5C. The two fragments remain associated with the virion. The enzymatic activity of the precursor is about 10% of that of mature gp5*.
Similarity Belongs to the glycosyl hydrolase 24 family.
else case <FTTag:Cleavage> and not <OC:Tequatrovirus>
Function [Mature baseplate central spike protein]: Baseplate central spike complex-associated lysozyme that is essential for the localized hydrolysis of bacterial cell wall, so that the tail tube, through which the phage DNA is ejected, can penetrate to the host inner membrane. The tail lysozyme complex at the tip of the tail tube penetrates through the outer membrane into the periplasm. This way, lysozyme domain is released and locally digests the peptidoglycan layer to make a hole to let the tube penetrate to the inner membrane. Involved in the tail assembly.
Catalytic activity [Mature baseplate central spike protein]: Reaction=Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins.; EC=3.2.1.17;
Subunit [Mature baseplate central spike protein]: Monomer. The central spike complex creates an extension of the tail tube.
[Baseplate central spike protein C-terminus]: Homotrimer. The central spike complex creates an extension of the tail tube.
[Pre-baseplate central spike protein]: Homotrimer.
Subcellular location [Mature baseplate central spike protein]: Virion. Note=Present in the baseplate.
[Baseplate central spike protein C-terminus]: Virion. Note=Present in the baseplate.
[Pre-baseplate central spike protein]: Virion. Note=Present in the baseplate.
Ptm [Pre-baseplate central spike protein]: In the fully assembled virus, the pre-baseplate central spike protein is cleaved to form the mature baseplate central spike protein, and a C-terminus fragment, the baseplate central spike protein C-terminus. The two fragments remain associated with the virion.
Caution The cleavage site between the mature baseplate central spike protein and the baseplate central spike protein C-terminus is uncertain.
Similarity Belongs to the glycosyl hydrolase 24 family.
else
Function [Baseplate central spike protein]: Baseplate central spike complex-associated lysozyme that is essential for the localized hydrolysis of bacterial cell wall, so that the tail tube, through which the phage DNA is ejected, can penetrate to the host inner membrane. The tail lysozyme complex at the tip of the tail tube penetrates through the outer membrane into the periplasm. This way, lysozyme domain is released and locally digests the peptidoglycan layer to make a hole to let the tube penetrate to the inner membrane. Involved in the tail assembly.
Catalytic activity [Baseplate central spike protein]: Reaction=Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins.; EC=3.2.1.17;
Similarity Belongs to the glycosyl hydrolase 24 family.
end case

Keywords [?]


Gene Ontology [?]

GO:0098015; Cellular component: virus tail.
GO:0098025; Cellular component: virus tail, baseplate.
GO:0003796; Molecular function: lysozyme activity.
GO:0098932; Biological process: disruption by virus of host cell wall peptidoglycan during virus entry.
GO:0009253; Biological process: peptidoglycan catabolic process.
GO:0098003; Biological process: viral tail assembly.

Cross-references [?]

Pfam PF06715; Gp5_C; 3;
PF06714; Gp5_OB; 1;
PF00959; Phage_lysozyme; 1;
PRINTS PR00684; T4LYSOZYME; 1;

Features [?]

From: NEEDLE_BPT4 (P16009)
Key     From     To       Description   Tag   Condition   FTGroup
ACT_SITE     184     184       Proton donor     E  
ACT_SITE     193     193       Nucleophile     D  
SITE (Optional)     351     352       Cleavage   Cleavage   S-A  
case <FTTag:Cleavage> and <OC:Tequatrovirus>
CHAIN     Nter     Cter       Pre-baseplate central spike protein Gp5        
CHAIN     Nter     351       Baseplate central spike protein Gp5*        
CHAIN     352     Cter       Gp5C        
else case <FTTag:Cleavage> and not <OC:Tequatrovirus>
CHAIN     Nter     Cter       Pre-baseplate central spike protein        
CHAIN     Nter     351       Mature baseplate central spike protein        
CHAIN     352     Cter       Baseplate central spike protein C-terminus        
else
CHAIN     Nter     Cter       Baseplate central spike protein        
end case

Additional information [?]

Size range 550-700 amino acids
Related rules None
Fusion None