Due to maintenance work, this service will be unavailable from Mon Nov 11 17:30 until Tue Nov 12 09:00 CET. Apologies for the inconvenience.

HAMAP rule MF_04151

Send feedback

General rule information [?]

Accession	MF_04151
Dates	15-JAN-2021 (Created) 1-MAR-2024 (Last updated, Version 4)
Name	NEEDLE_T4
Scope(s)	Viruses
Template(s)	P16009 (NEEDL_BPT4); [ Recover all ]
Triggered by	HAMAP; MF_04151 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier	NEEDL
case <FTTag:Cleavage> and <OC:Tequatrovirus>
Protein name	RecName: Full=Pre-baseplate central spike protein Gp5; Short=Pre-Gp5; AltName: Full=Peptidoglycan hydrolase gp5; EC=3.2.1.17; Contains: RecName: Full=Baseplate central spike protein Gp5*; AltName: Full=Mature Gp5; Contains: RecName: Full=Gp5C;
Gene name	Name=5;
else case <FTTag:Cleavage> and not <OC:Tequatrovirus>
Protein name	RecName: Full=Pre-baseplate central spike protein; AltName: Full=Peptidoglycan hydrolase; EC=3.2.1.17; Contains: RecName: Full=Mature baseplate central spike protein; Contains: RecName: Full=Baseplate central spike protein C-terminus;
else
Protein name	RecName: Full=Baseplate central spike protein; AltName: Full=Peptidoglycan hydrolase; EC=3.2.1.17;
end case

Comments [?]

case <FTTag:Cleavage> and <OC:Tequatrovirus>
FUNCTION	[Baseplate central spike protein Gp5]: Baseplate central spike complex-associated lysozyme that is essential for the localized hydrolysis of bacterial cell wall, so that the tail tube, through which the phage DNA is ejected, can penetrate to the host inner membrane. The tail lysozyme complex at the tip of the tail tube penetrates through the outer membrane into the periplasm and during that process, gp5 dissociates from gp5C and activated. Due to the lower pH in the periplasm, gp5* would dissociate from gp27 which probably still binds to the tip of the tube. This way, lysozyme domain is released and locally digests the peptidoglycan layer to make a hole to let the tube penetrate to the inner membrane. Involved in the tail assembly.
CATALYTIC ACTIVITY	[Baseplate central spike protein Gp5*]: Reaction=Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins.; EC=3.2.1.17;
SUBUNIT	[Baseplate central spike protein Gp5]: Monomer. The central spike complex, which creates an extension of the tail tube, is made up of three copies of the gp27-gp5-gp5C complex and one copy of gp5.4. Part of the baseplate macromolecular complex which consists of gp5*, gp5C, gp5.4, gp27 (central spike complex); gp6, gp25, gp53 (inner baseplate); gp7, gp8 (intermediate baseplate); gp9, gp10, gp11, gp12 (peripheral); gp48 and gp54 (proximal region of the tail tube).
SUBUNIT	[Gp5C]: Homotrimer. The central spike complex, which creates an extension of the tail tube, is made up of three copies of the gp27- gp5-gp5C complex and one copy of gp5.4. Part of the baseplate macromolecular complex which consists of gp5, gp5C, gp5.4, gp27 (central spike complex); gp6, gp25, gp53 (inner baseplate); gp7, gp8 (intermediate baseplate); gp9, gp10, gp11, gp12 (peripheral); gp48 and gp54 (proximal region of the tail tube).
SUBUNIT	[Pre-baseplate central spike protein Gp5]: Homotrimer.
SUBCELLULAR LOCATION	[Baseplate central spike protein Gp5*]: Virion. Note=Present in 3 copies in the baseplate.
SUBCELLULAR LOCATION	[Gp5C]: Virion. Note=Present in 3 copies in the baseplate.
SUBCELLULAR LOCATION	[Pre-baseplate central spike protein Gp5]: Virion. Note=Present in the baseplate.
PTM	[Pre-baseplate central spike protein Gp5]: In the fully assembled virus, gp5 precursor is cleaved to form the mature tail lysozyme gp5, and a C-terminus fragment, gp5C. The two fragments remain associated with the virion. The enzymatic activity of the precursor is about 10% of that of mature gp5.
SIMILARITY	Belongs to the glycosyl hydrolase 24 family.
else case <FTTag:Cleavage> and not <OC:Tequatrovirus>
FUNCTION	[Mature baseplate central spike protein]: Baseplate central spike complex-associated lysozyme that is essential for the localized hydrolysis of bacterial cell wall, so that the tail tube, through which the phage DNA is ejected, can penetrate to the host inner membrane. The tail lysozyme complex at the tip of the tail tube penetrates through the outer membrane into the periplasm. This way, lysozyme domain is released and locally digests the peptidoglycan layer to make a hole to let the tube penetrate to the inner membrane. Involved in the tail assembly.
CATALYTIC ACTIVITY	[Mature baseplate central spike protein]: Reaction=Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins.; EC=3.2.1.17;
SUBUNIT	[Mature baseplate central spike protein]: Monomer. The central spike complex creates an extension of the tail tube.
SUBUNIT	[Baseplate central spike protein C-terminus]: Homotrimer. The central spike complex creates an extension of the tail tube.
SUBUNIT	[Pre-baseplate central spike protein]: Homotrimer.
SUBCELLULAR LOCATION	[Mature baseplate central spike protein]: Virion. Note=Present in the baseplate.
SUBCELLULAR LOCATION	[Baseplate central spike protein C-terminus]: Virion. Note=Present in the baseplate.
SUBCELLULAR LOCATION	[Pre-baseplate central spike protein]: Virion. Note=Present in the baseplate.
PTM	[Pre-baseplate central spike protein]: In the fully assembled virus, the pre-baseplate central spike protein is cleaved to form the mature baseplate central spike protein, and a C-terminus fragment, the baseplate central spike protein C-terminus. The two fragments remain associated with the virion.
CAUTION	The cleavage site between the mature baseplate central spike protein and the baseplate central spike protein C-terminus is uncertain.
SIMILARITY	Belongs to the glycosyl hydrolase 24 family.
else
FUNCTION	[Baseplate central spike protein]: Baseplate central spike complex-associated lysozyme that is essential for the localized hydrolysis of bacterial cell wall, so that the tail tube, through which the phage DNA is ejected, can penetrate to the host inner membrane. The tail lysozyme complex at the tip of the tail tube penetrates through the outer membrane into the periplasm. This way, lysozyme domain is released and locally digests the peptidoglycan layer to make a hole to let the tube penetrate to the inner membrane. Involved in the tail assembly.
CATALYTIC ACTIVITY	[Baseplate central spike protein]: Reaction=Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins.; EC=3.2.1.17;
SIMILARITY	Belongs to the glycosyl hydrolase 24 family.
end case