HAMAP rule MF_04154

General rule information [?]

Accession	MF_04154
Dates	8-FEB-2021 (Created) 19-NOV-2022 (Last updated, Version 6)
Name	Helic_Prim_T7
Scope(s)	Viruses
Template(s)	P03692 (HELIC_BPT7); [ Recover all ]
Triggered by	HAMAP; MF_04154 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier	HELIC
RecName: Full=DNA helicase/primase;<br />                  EC=<a href="https://enzyme.expasy.org/EC/2.7.7.-">2.7.7.-</a>;<br />                  EC=<a href="https://enzyme.expasy.org/EC/3.6.4.12">3.6.4.12</a>;
case <OC:Teseptimavirus>
Protein name	AltName: Full=Gene product 4; Short=Gp4;
Gene name	Name=4;
end case

Comments [?]

case <OC:Teseptimavirus>
FUNCTION	ATP-dependent DNA helicase and primase essential for viral DNA replication and recombination. The helicase moves 5' -> 3' on the lagging strand template, unwinding the DNA duplex ahead of the leading strand polymerase at the replication fork and generating ssDNA for both leading and lagging strand synthesis. ATP or dTTP hydrolysis propels each helicase domain to translocate 2 nt per step sequentially along DNA. Mediates strand transfer when a joint molecule is available and participates in recombinational DNA repair through its role in strand exchange. Primase activity synthesizes short RNA primers at the sequence 5'-GTC-3' on the lagging strand that the polymerase elongates using dNTPs and providing the primase is still present.
else
FUNCTION	ATP-dependent DNA helicase and primase essential for viral DNA replication and recombination. The helicase moves 5' -> 3' on the lagging strand template, unwinding the DNA duplex ahead of the leading strand polymerase at the replication fork and generating ssDNA for both leading and lagging strand synthesis. ATP or dTTP hydrolysis propels each helicase domain to translocate sequentially along DNA. Mediates strand transfer when a joint molecule is available and participates in recombinational DNA repair through its role in strand exchange. Primase activity synthesizes short RNA primers at the sequence 5'-GTC-3' on the lagging strand that the polymerase elongates using dNTPs and providing the primase is still present.
end case
CATALYTIC ACTIVITY	Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12;
COFACTOR	Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Note=Binds 2 Mg(2+), one of which is catalytic.;
case <OC:Teseptimavirus>
SUBUNIT	Homohexamer. Assembles as a hexamer onto linear or circular ssDNA in the presence of ATP or dTTP. Interacts (via C-terminus) with the viral DNA polymerase that is bound to DNA; this interaction is essential to initiate leading-strand DNA synthesis. The priming complex consists of 2 DNA polymerases and 1 helicase-primase hexamer that assemble on the DNA template. Interacts with the single-stranded DNA- binding protein. Part of the replicase complex that includes the DNA polymerase, thioredoxin, the primase/helicase and the single-stranded DNA binding protein.
else
SUBUNIT	Homohexamer. Assembles as a hexamer onto linear or circular ssDNA in the presence of ATP or dTTP. Interacts (via C-terminus) with the viral DNA polymerase that is bound to DNA; this interaction is essential to initiate leading-strand DNA synthesis. The priming complex consists of 2 DNA polymerases and 1 helicase-primase hexamer that assemble on the DNA template. Interacts with the single-stranded DNA- binding protein. Part of the replicase complex that includes the DNA polymerase, the primase/helicase and the single-stranded DNA binding protein.
end case
case <FTGroup:1> and <FTGroup:2>
DOMAIN	The N-terminus zinc finger domain is essential for delivering the primed DNA template to the DNA polymerase. The central core domain contains the primase activity. The C-terminus region is responsible for the helicase activity and binds 1 Mg(2+)-dTTP.
else case <FTGroup:2> and not <FTGroup:1>
DOMAIN	The central core domain contains the primase activity. The C- terminus is responsible for the helicase activity.
end case
SIMILARITY	Belongs to the Teseptimavirus DNA helicase/primase family.

Keywords [?]

ATP-binding
Viral DNA replication
<a href="https://www.uniprot.org/keywords/KW-0347">Helicase</a>
Hydrolase
Nucleotidyltransferase
Primosome
Metal-binding
Multifunctional enzyme
Transferase
Zinc-finger
Zinc
case <FTGroup:2>
Magnesium
end case

Gene Ontology [?]

GO:0003896; Molecular function:DNA primase activity
case <FTGroup:1>
GO:0008270; Molecular function:zinc ion binding
end case
GO:0005524; Molecular function:ATP binding
GO:0003678; Molecular function:DNA helicase activity
GO:0039693; Biological process:viral DNA genome replication
GO:0016740; Molecular function:transferase activity

Cross-references [?]

PROSITE	PS50880; TOPRIM; 1;
PROSITE	PS51199; SF4_HELICASE; 1;
Pfam	PF03796; DnaB_C; 1;
Pfam	PF08273; Prim_Zn_Ribbon; 0-1;

Features [?]

From: HELIC_BPT7 (P03692)
Key	From	To	Description	Tag	Condition	FTGroup
ZN_FING	17	39	/note="C4-like; zinc ribbon fold"		C-x(2,4)-C-x(15,21)-C-x(2)-C
BINDING	312	319	/ligand="ATP" /ligand_id="ChEBI:CHEBI:30616"		[AS]-G-[ST]-G-x-G-K-[ST]
REGION	550	566	/note="Binding to viral DNA polymerase"
BINDING	17	17	/ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105"		C	1
BINDING	20	20	/ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105"		C	1
BINDING	36	36	/ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105"		C	1
BINDING	39	39	/ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105"		C	1
BINDING	157	157	/ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" /ligand_label="1" /ligand_note="catalytic"		E	2
BINDING	207	207	/ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" /ligand_label="1" /ligand_note="catalytic"		D	2
BINDING	237	237	/ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" /ligand_label="2"		D
SITE	361	361	/note="dTTP/dATP binding"		R
SITE	465	465	/note="dTTP/dATP binding"		H
SITE	504	504	/note="dTTP/dATP binding"		R
SITE	522	522	/note="dTTP/dATP binding"		R
SITE	535	535	/note="dTTP/dATP binding"		Y

Additional information [?]

Size range	460-650 amino acids
Related rules	None
Fusion	Nter: None Cter: None

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