HAMAP rule MF_04157
General rule information
[?]
| Accession | MF_04157 |
| Dates | 26-FEB-2021 (Created)
19-NOV-2022 (Last updated, Version 5) |
| Name | PRIMASE_T4 |
| Scope(s) |
Viruses Enterobacteria phage T4 |
| Template(s) | P04520 (PRIM_BPT4); [ Recover all ] |
| Triggered by |
HAMAP; MF_04157 (Get profile general information and statistics) |
Propagated annotation
[?]
Identifier, protein and gene names
[?]
| Identifier | PRIM |
| Protein name | RecName: Full=DNA primase; EC=2.7.7.-; AltName: Full=DNA priming protein; |
Comments
[?]
| FUNCTION | Synthesizes short RNA primers for the lagging strand DNA replication. The primase synthesizes short RNA primers on the lagging strand that the polymerase elongates using dNTPs. Recognizes two trinucleotide sequences 5'-GTT-3' and 5'-GCT-3' in vitro, but uses only the first as the priming site in vivo. |
| SUBUNIT | Monomer. Hexamer. Interacts with the DnaB-like replicative helicase; this interaction forms the active primosome complex, which is composed of 6 helicase and 1 primase subunits and expresses full helicase and primase activities. Interacts (via C-terminus) with the single-stranded DNA-binding protein. Part of the replicase complex that includes the DNA polymerase, the polymerase clamp, the clamp loader complex, the single-stranded DNA binding protein, the primase, the DnaB-like replicative helicase and the helicase assembly factor. |
| SIMILARITY | Belongs to the Tequatrovirus DNA primase family. |
Keywords
[?]
| Viral DNA replication | |
| Early protein | |
| Transferase | |
| case <FTGroup:1> | |
| Zinc | |
| end case | |
Gene Ontology
[?]
| GO:0039693; Biological process:viral DNA genome replication | |
| GO:0016740; Molecular function:transferase activity | |
| GO:0003896; Molecular function:DNA primase activity | |
| case <FTGroup:1> | |
| GO:0008270; Molecular function:zinc ion binding | |
| end case | |
Cross-references
[?]
| Pfam | PF08275; Toprim_N; 1; |
Features
[?]
| From: PRIM_BPT4 (P04520) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| BINDING | 37 | 37 | /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" |
C | 1 | |||||||
| BINDING | 40 | 40 | /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" |
C | 1 | |||||||
| BINDING | 65 | 65 | /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" |
C | 1 | |||||||
| BINDING | 68 | 68 | /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" |
C | 1 | |||||||
Additional information
[?]
| Size range | 260-460 amino acids |
| Related rules |
None |
| Fusion | Nter: None Cter: None |