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HAMAP rule MF_04157

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General rule information [?]

Accession MF_04157
Dates 26-FEB-2021 (Created)
19-NOV-2022 (Last updated, Version 5)
Scope(s) Viruses
Enterobacteria phage T4
Template(s) P04520 (PRIM_BPT4); [ Recover all ]
Triggered by HAMAP; MF_04157 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier PRIM
Protein name RecName: Full=DNA primase;
AltName: Full=DNA priming protein;

Comments [?]

FUNCTIONSynthesizes short RNA primers for the lagging strand DNA replication. The primase synthesizes short RNA primers on the lagging strand that the polymerase elongates using dNTPs. Recognizes two trinucleotide sequences 5'-GTT-3' and 5'-GCT-3' in vitro, but uses only the first as the priming site in vivo.
SUBUNITMonomer. Hexamer. Interacts with the DnaB-like replicative helicase; this interaction forms the active primosome complex, which is composed of 6 helicase and 1 primase subunits and expresses full helicase and primase activities. Interacts (via C-terminus) with the single-stranded DNA-binding protein. Part of the replicase complex that includes the DNA polymerase, the polymerase clamp, the clamp loader complex, the single-stranded DNA binding protein, the primase, the DnaB-like replicative helicase and the helicase assembly factor.
SIMILARITYBelongs to the Tequatrovirus DNA primase family.

Keywords [?]

Gene Ontology [?]

GO:0039693; Biological process:viral DNA genome replication
GO:0016740; Molecular function:transferase activity
GO:0003896; Molecular function:DNA primase activity
case <FTGroup:1>
GO:0008270; Molecular function:zinc ion binding
end case

Cross-references [?]

Pfam PF08275; Toprim_N; 1;

Features [?]

From: PRIM_BPT4 (P04520)
Key From To Description Tag Condition FTGroup
BINDING 37 37 /ligand="Zn(2+)"
C 1
BINDING 40 40 /ligand="Zn(2+)"
C 1
BINDING 65 65 /ligand="Zn(2+)"
C 1
BINDING 68 68 /ligand="Zn(2+)"
C 1

Additional information [?]

Size range 260-460 amino acids
Related rules None
Fusion Nter: None Cter: None

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