Home  |  Contact
Annotation rule MF_04157
Send feedback

General rule information [?]

Accession MF_04157
Dates 26-FEB-2021 (Created)
31-MAR-2021 (Last updated, Version 2)
Viruses; Enterobacteria phage T4
Template P04520 (PRIM_BPT4)

Propagated annotation [?]

Identifier, protein and gene names [?]

Protein name
RecName: Full=DNA primase;
EC 2.7.7.-;
AltName: Full=DNA priming protein;

Comments [?]

Function Synthesizes short RNA primers for the lagging strand DNA replication. The primase synthesizes short RNA primers on the lagging strand that the polymerase elongates using dNTPs. Recognizes two trinucleotide sequences 5'-GTT-3' and 5'-GCT-3' in vitro, but uses only the first as the priming site in vivo.
Subunit Monomer. Hexamer. Interacts with the DnaB-like replicative helicase; this interaction forms the active primosome complex, which is composed of 6 helicase and 1 primase subunits and expresses full helicase and primase activities. Interacts (via C-terminus) with the single-stranded DNA-binding protein. Part of the replicase complex that includes the DNA polymerase, the polymerase clamp, the clamp loader complex, the single-stranded DNA binding protein, the primase, the DnaB-like replicative helicase and the helicase assembly factor.
Similarity Belongs to the Tequatrovirus DNA primase family.

Keywords [?]

case <FTTag:Zinc>
end case

Gene Ontology [?]

GO:0039693; Biological process: viral DNA genome replication.
GO:0016740; Molecular function: transferase activity.
GO:0003896; Molecular function: DNA primase activity.
case <FTTag:Zinc>
GO:0008270; Molecular function: zinc ion binding.
end case

Cross-references [?]

Pfam PF08275; Toprim_N; 1;

Features [?]

From: PRIM_BPT4 (P04520)
Key     From     To       Description   Tag   Condition   FTGroup
REGION     37     68       Zinc-binding   Zinc   C-x(2,4)-C-x(20,25)-C-x(2)-C  

Additional information [?]

Size range 260-460 amino acids
Related rules None
Fusion None