HAMAP rule MF_04200
General rule information
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| Accession | MF_04200 |
| Dates | 22-MAR-2018 (Created)
29-SEP-2022 (Last updated, Version 5) |
| Name | ALPHA_CORONA_SPIKE |
| Scope(s) |
Viruses Alphacoronavirus |
| Template(s) | P15423 (SPIKE_CVH22); [ Recover all ] |
| Triggered by |
HAMAP; MF_04200 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | SPIKE |
| Protein name | RecName: Full=Spike glycoprotein; Short=S glycoprotein; AltName: Full=E2; AltName: Full=Peplomer protein; Flags: Precursor; |
| Gene name | Name=S; |
Comments
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| FUNCTION | S1 region attaches the virion to the cell membrane by interacting with the host receptor, initiating the infection. Binding to the receptor probably induces conformational changes in the S glycoprotein unmasking the fusion peptide of S2 region and activating membranes fusion. S2 region belongs to the class I viral fusion protein. Under the current model, the protein has at least 3 conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats) regions assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes. |
| SUBUNIT | Homotrimer. During virus morphogenesis, found in a complex with M and HE proteins. Interacts with host receptor. |
| SUBCELLULAR LOCATION | Virion membrane; Single-pass type I membrane protein. Host endoplasmic reticulum-Golgi intermediate compartment membrane; Single-pass type I membrane protein. Note=Accumulates in the endoplasmic reticulum-Golgi intermediate compartment, where it participates in virus particle assembly. |
| DOMAIN | The KxHxx motif seems to function as an ER retrieval signal. |
| SIMILARITY | Belongs to the alphacoronaviruses spike protein family. |
| CAUTION | In contrast to serogroups 2 and 3, S glycoprotein from serogroup 1 is not cleaved into S1 and S2. |
Keywords
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Gene Ontology
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| GO:0044173; Cellular component:host cell endoplasmic reticulum-Golgi intermediate compartment membrane |
| GO:0016020; Cellular component:membrane |
| GO:0019031; Cellular component:viral envelope |
| GO:0055036; Cellular component:virion membrane |
| GO:0075509; Biological process:endocytosis involved in viral entry into host cell |
| GO:0039654; Biological process:fusion of virus membrane with host endosome membrane |
| GO:0019064; Biological process:fusion of virus membrane with host plasma membrane |
| GO:0046813; Biological process:receptor-mediated virion attachment to host cell |
Cross-references
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| Pfam | PF01600; Corona_S1; 1; |
| Pfam | PF01601; Corona_S2; 1; |
| General | Coiled_coil; -; 2-unlimited; |
| General | Signal; -; 1; |
| General | Transmembrane; -; 1; |
Features
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| From: SPIKE_CVH22 (P15423) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| SIGNAL | Nter | 15 | ||||||||||
| CHAIN | 16 | 1173 | /note="Spike glycoprotein" | |||||||||
| TOPO_DOM | 16 | 1115 | /note="Virion surface" | |||||||||
| TRANSMEM | 1116 | 1135 | /note="Helical" | |||||||||
| TOPO_DOM | 1136 | 1173 | /note="Intravirion" | |||||||||
| REGION | 16 | 536 | /note="S1" | |||||||||
| REGION | 537 | 1173 | /note="S2" | |||||||||
| REGION | 753 | 773 | /note="Fusion peptide" | |||||||||
| COILED | 834 | 878 | ||||||||||
| COILED | 1063 | 1105 | ||||||||||
| MOTIF | 1169 | 1173 | /note="KxHxx" | K-x-H-x-x | ||||||||
Additional information
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| Size range | 1173-1453 amino acids |
| Related rules |
None |
| Fusion | Nter: None Cter: None |