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Annotation rule MF_04200 |
Accession | MF_04200 |
Dates | 22-MAR-2018 (Created) 20-NOV-2019 (Last updated, Version 3) |
Name | ALPHA_CORONA_SPIKE |
Scope | Viruses; Alphacoronavirus |
Template | P15423 (SPIKE_CVH22) |
Triggered by |
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Function | S1 region attaches the virion to the cell membrane by interacting with host ANPEP/aminopeptidase N, initiating the infection. Binding to the receptor probably induces conformational changes in the S glycoprotein unmasking the fusion peptide of S2 region and activating membranes fusion. S2 region belongs to the class I viral fusion protein. Under the current model, the protein has at least 3 conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats) regions assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes. |
Subunit | Homotrimer. During virus morphogenesis, found in a complex with M and HE proteins. Interacts with host ANPEP. |
Subcellular location | Virion membrane; Single-pass type I membrane protein. Host endoplasmic reticulum-Golgi intermediate compartment membrane; Single-pass type I membrane protein. Note=Accumulates in the endoplasmic reticulum-Golgi intermediate compartment, where it participates in virus particle assembly. |
Domain | The KxHxx motif seems to function as an ER retrieval signal. |
Similarity | Belongs to the alphacoronaviruses spike protein family. |
Caution | In contrast to serogroups 2 and 3, S glycoprotein from serogroup 1 is not cleaved into S1 and S2. |
General | Coiled_coil; -; 2-unlimited; |
Signal; -; 1; | |
Transmembrane; -; 1; |
From: SPIKE_CVH22 (P15423) | ||||||||||||
Key | From | To | Description | Tag | Condition | FTGroup | ||||||
SIGNAL | Nter | 15 | ||||||||||
CHAIN | 16 | 1173 | Spike glycoprotein | |||||||||
TOPO_DOM | 16 | 1115 | Virion surface | |||||||||
TRANSMEM | 1116 | 1135 | Helical | |||||||||
TOPO_DOM | 1136 | 1173 | Intravirion | |||||||||
REGION | 16 | 536 | S1 | |||||||||
REGION | 417 | 547 | Interaction with host ANPEP | |||||||||
REGION | 537 | 1173 | S2 | |||||||||
REGION | 753 | 773 | Fusion peptide | |||||||||
COILED | 834 | 878 | ||||||||||
COILED | 1063 | 1105 | ||||||||||
MOTIF | 1169 | 1173 | KxHxx | K-x-H-x-x |
Size range | 1173-1453 amino acids |
Related rules | None |
Fusion | None |