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HAMAP rule MF_04200

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General rule information [?]

Accession MF_04200
Dates 22-MAR-2018 (Created)
29-SEP-2022 (Last updated, Version 5)
Scope(s) Viruses
Template(s) P15423 (SPIKE_CVH22); [ Recover all ]
Triggered by HAMAP; MF_04200 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier SPIKE
Protein name RecName: Full=Spike glycoprotein;
                 Short=S glycoprotein;
AltName: Full=E2;
AltName: Full=Peplomer protein;
                 Flags: Precursor;
Gene name Name=S;

Comments [?]

FUNCTIONS1 region attaches the virion to the cell membrane by interacting with the host receptor, initiating the infection. Binding to the receptor probably induces conformational changes in the S glycoprotein unmasking the fusion peptide of S2 region and activating membranes fusion. S2 region belongs to the class I viral fusion protein. Under the current model, the protein has at least 3 conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats) regions assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes.
SUBUNITHomotrimer. During virus morphogenesis, found in a complex with M and HE proteins. Interacts with host receptor.
SUBCELLULAR LOCATIONVirion membrane; Single-pass type I membrane protein. Host endoplasmic reticulum-Golgi intermediate compartment membrane; Single-pass type I membrane protein. Note=Accumulates in the endoplasmic reticulum-Golgi intermediate compartment, where it participates in virus particle assembly.
DOMAINThe KxHxx motif seems to function as an ER retrieval signal.
SIMILARITYBelongs to the alphacoronaviruses spike protein family.
CAUTIONIn contrast to serogroups 2 and 3, S glycoprotein from serogroup 1 is not cleaved into S1 and S2.

Keywords [?]

Gene Ontology [?]

GO:0044173; Cellular component:host cell endoplasmic reticulum-Golgi intermediate compartment membrane
GO:0016020; Cellular component:membrane
GO:0019031; Cellular component:viral envelope
GO:0055036; Cellular component:virion membrane
GO:0075509; Biological process:endocytosis involved in viral entry into host cell
GO:0039654; Biological process:fusion of virus membrane with host endosome membrane
GO:0019064; Biological process:fusion of virus membrane with host plasma membrane
GO:0046813; Biological process:receptor-mediated virion attachment to host cell

Cross-references [?]

Pfam PF01600; Corona_S1; 1;
Pfam PF01601; Corona_S2; 1;
General Coiled_coil; -; 2-unlimited;
General Signal; -; 1;
General Transmembrane; -; 1;

Features [?]

From: SPIKE_CVH22 (P15423)
Key From To Description Tag Condition FTGroup
SIGNAL Nter 15
CHAIN 16 1173 /note="Spike glycoprotein"
TOPO_DOM 16 1115 /note="Virion surface"
TRANSMEM 1116 1135 /note="Helical"
TOPO_DOM 1136 1173 /note="Intravirion"
REGION 16 536 /note="S1"
REGION 537 1173 /note="S2"
REGION 753 773 /note="Fusion peptide"
COILED 834 878
COILED 1063 1105
MOTIF 1169 1173 /note="KxHxx" K-x-H-x-x

Additional information [?]

Size range 1173-1453 amino acids
Related rules None
Fusion Nter: None Cter: None

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