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Annotation rule MF_04200
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General rule information [?]

Accession MF_04200
Dates 22-MAR-2018 (Created)
20-NOV-2019 (Last updated, Version 3)
Viruses; Alphacoronavirus
Template P15423 (SPIKE_CVH22)

Propagated annotation [?]

Identifier, protein and gene names [?]

Protein name
RecName: Full=Spike glycoprotein;
Short=S glycoprotein;
AltName: Full=E2;
AltName: Full=Peplomer protein;
Flags: Precursor;
Gene name

Comments [?]

Function S1 region attaches the virion to the cell membrane by interacting with host ANPEP/aminopeptidase N, initiating the infection. Binding to the receptor probably induces conformational changes in the S glycoprotein unmasking the fusion peptide of S2 region and activating membranes fusion. S2 region belongs to the class I viral fusion protein. Under the current model, the protein has at least 3 conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats) regions assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes.
Subunit Homotrimer. During virus morphogenesis, found in a complex with M and HE proteins. Interacts with host ANPEP.
Subcellular location Virion membrane; Single-pass type I membrane protein. Host endoplasmic reticulum-Golgi intermediate compartment membrane; Single-pass type I membrane protein. Note=Accumulates in the endoplasmic reticulum-Golgi intermediate compartment, where it participates in virus particle assembly.
Domain The KxHxx motif seems to function as an ER retrieval signal.
Similarity Belongs to the alphacoronaviruses spike protein family.
Caution In contrast to serogroups 2 and 3, S glycoprotein from serogroup 1 is not cleaved into S1 and S2.

Keywords [?]

Gene Ontology [?]

GO:0044173; Cellular component: host cell endoplasmic reticulum-Golgi intermediate compartment membrane.
GO:0016021; Cellular component: integral component of membrane.
GO:0019031; Cellular component: viral envelope.
GO:0055036; Cellular component: virion membrane.
GO:0075509; Biological process: endocytosis involved in viral entry into host cell.
GO:0039654; Biological process: fusion of virus membrane with host endosome membrane.
GO:0019064; Biological process: fusion of virus membrane with host plasma membrane.
GO:0046813; Biological process: receptor-mediated virion attachment to host cell.

Cross-references [?]

Pfam PF01600; Corona_S1; 1;
PF01601; Corona_S2; 1;

Computed features [?]

General Coiled_coil; -; 2-unlimited;
Signal; -; 1;
Transmembrane; -; 1;

Features [?]

From: SPIKE_CVH22 (P15423)
Key     From     To       Description   Tag   Condition   FTGroup
SIGNAL     Nter     15                
CHAIN     16     1173       Spike glycoprotein        
TOPO_DOM     16     1115       Virion surface        
TRANSMEM     1116     1135       Helical        
TOPO_DOM     1136     1173       Intravirion        
REGION     16     536       S1        
REGION     417     547       Interaction with host ANPEP        
REGION     537     1173       S2        
REGION     753     773       Fusion peptide        
COILED     834     878                
COILED     1063     1105                
MOTIF     1169     1173       KxHxx     K-x-H-x-x  

Additional information [?]

Size range 1173-1453 amino acids
Related rules None
Fusion None