HAMAP rule MF_00130
General rule information
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| Accession | MF_00130 |
| Dates | 1-JUN-2001 (Created)
1-JUN-2023 (Last updated, Version 30) |
| Name | RecU |
| Scope(s) |
Bacteria |
| Template(s) | P39792 (RECU_BACSU); Q5KXY4 (RECU_GEOKA); [ Recover all ] |
| Triggered by |
HAMAP; MF_00130 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | RECU |
| Protein name | RecName: Full=Holliday junction resolvase RecU; EC=3.1.21.10; AltName: Full=Recombination protein U homolog; |
| Gene name | Name=recU; |
Comments
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| FUNCTION | Endonuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves mobile four-strand junctions by introducing symmetrical nicks in paired strands. Promotes annealing of linear ssDNA with homologous dsDNA. Required for DNA repair, homologous recombination and chromosome segregation. |
| CATALYTIC ACTIVITY | Reaction=Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction).; EC=3.1.21.10; |
| COFACTOR | Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Note=Binds 1 Mg(2+) ion per subunit.; |
| SUBCELLULAR LOCATION | Cytoplasm. |
| SIMILARITY | Belongs to the RecU family. |
Keywords
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| Cytoplasm |
| DNA damage |
| DNA repair |
| DNA recombination |
| Endonuclease |
| Hydrolase |
| Magnesium |
| Metal-binding |
| Nuclease |
Gene Ontology
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| GO:0004519; Molecular function:endonuclease activity |
| GO:0000287; Molecular function:magnesium ion binding |
| GO:0007059; Biological process:chromosome segregation |
| GO:0006310; Biological process:DNA recombination |
| GO:0006281; Biological process:DNA repair |
| GO:0005737; Cellular component:cytoplasm |
Cross-references
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Features
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| From: RECU_GEOKA (Q5KXY4) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| BINDING | 84 | 84 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" |
T | ||||||||
| BINDING | 86 | 86 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" |
D | ||||||||
| BINDING | 99 | 99 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" |
[DE] | ||||||||
| BINDING | 118 | 118 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" |
Q | ||||||||
| SITE | 101 | 101 | /note="Transition state stabilizer" | K | ||||||||
Additional information
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| Size range | 157-213 amino acids |
| Related rules |
None |
| Fusion | Nter: None Cter: None |