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HAMAP rule MF_00295

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General rule information [?]

Accession MF_00295
Dates 1-JUN-2001 (Created)
1-JUN-2023 (Last updated, Version 35)
Name MetA_acyltransf
Scope(s) Bacteria
Template(s) Q72X44 (METAA_BACC1); P07623 (METAS_ECOLI); Q9WZY3 (METAA_THEMA); A0A1D3PCK2 (SAT_LACAI); [ Recover all ]
Triggered by HAMAP; MF_00295 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

case <FTGroup:1> and ((<FTTag:P4> and <FTTag:P7>) or <FTTag:P5a>)
Identifier METAS
Protein name RecName: Full=Homoserine O-succinyltransferase;
                 Short=HST;
                 EC=2.3.1.46;
AltName: Full=Homoserine transsuccinylase;
                 Short=HTS;
Gene name Name=metAS;
else case <FTGroup:1> and <FTTag:P5b> and <FTTag:P10b> and not (<FTTag:P4> or <FTTag:P7>)
Identifier METAA
Protein name RecName: Full=Homoserine O-acetyltransferase;
                 Short=HAT;
                 EC=2.3.1.31;
AltName: Full=Homoserine transacetylase;
                 Short=HTA;
Gene name Name=metAA;
else case <FTGroup:1> and <FTTag:P5b> and <FTTag:P10a> and not (<FTTag:P4> or <FTTag:P7>)
Identifier SAT
Protein name RecName: Full=Serine O-acetyltransferase;
                 Short=SAT;
                 EC=2.3.1.30;
else
Identifier META
Protein name RecName: Full=Probable acyltransferase;
                 EC=2.3.1.-;
end case

Comments [?]

case <FTGroup:1> and ((<FTTag:P4> and <FTTag:P7>) or <FTTag:P5a>)
FUNCTIONTransfers a succinyl group from succinyl-CoA to L-homoserine, forming succinyl-L-homoserine.
CATALYTIC ACTIVITY Reaction=L-homoserine + succinyl-CoA = CoA + O-succinyl-L-homoserine; Xref=Rhea:RHEA:22008, ChEBI:CHEBI:57287, ChEBI:CHEBI:57292, ChEBI:CHEBI:57476, ChEBI:CHEBI:57661; EC=2.3.1.46;
PATHWAYAmino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; O-succinyl-L-homoserine from L-homoserine: step 1/1.
else case <FTGroup:1> and <FTTag:P5b> and <FTTag:P10b> and not (<FTTag:P4> or <FTTag:P7>)
FUNCTIONTransfers an acetyl group from acetyl-CoA to L-homoserine, forming acetyl-L-homoserine.
CATALYTIC ACTIVITY Reaction=acetyl-CoA + L-homoserine = CoA + O-acetyl-L-homoserine; Xref=Rhea:RHEA:13701, ChEBI:CHEBI:57287, ChEBI:CHEBI:57288, ChEBI:CHEBI:57476, ChEBI:CHEBI:57716; EC=2.3.1.31;
PATHWAYAmino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; O-acetyl-L-homoserine from L-homoserine: step 1/1.
else case <FTGroup:1> and <FTTag:P5b> and <FTTag:P10a> and not (<FTTag:P4> or <FTTag:P7>)
FUNCTIONTransfers an acetyl group from acetyl-CoA to L-serine, forming acetyl-L-serine.
CATALYTIC ACTIVITY Reaction=acetyl-CoA + L-serine = CoA + O-acetyl-L-serine; Xref=Rhea:RHEA:24560, ChEBI:CHEBI:33384, ChEBI:CHEBI:57287, ChEBI:CHEBI:57288, ChEBI:CHEBI:58340; EC=2.3.1.30;
PATHWAYAmino-acid biosynthesis; L-cysteine biosynthesis; L-cysteine from L-serine: step 1/2.
end case
case <OC:Escherichia> or <OC:Shigella> or <OC:Salmonella>
SUBUNITHomodimer.
end case
SUBCELLULAR LOCATIONCytoplasm.
SIMILARITYBelongs to the MetA family.

Keywords [?]

Cytoplasm
Amino-acid biosynthesis
case <FTGroup:1> and ((<FTTag:P4> and <FTTag:P7>) or <FTTag:P5a>)
Methionine biosynthesis
else case <FTGroup:1> and <FTTag:P5b> and <FTTag:P10b> and not (<FTTag:P4> or <FTTag:P7>)
Methionine biosynthesis
else case <FTGroup:1> and <FTTag:P5b> and <FTTag:P10a> and not (<FTTag:P4> or <FTTag:P7>)
Cysteine biosynthesis
end case
Transferase
Acyltransferase

Gene Ontology [?]

case <FTGroup:1> and ((<FTTag:P4> and <FTTag:P7>) or <FTTag:P5a>)
GO:0004414; Molecular function:homoserine O-acetyltransferase activity
GO:0009086; Biological process:methionine biosynthetic process
else case <FTGroup:1> and <FTTag:P5b> and <FTTag:P10b> and not (<FTTag:P4> or <FTTag:P7>)
GO:0008899; Molecular function:homoserine O-succinyltransferase activity
GO:0009086; Biological process:methionine biosynthetic process
else case <FTGroup:1> and <FTTag:P5b> and <FTTag:P10a> and not (<FTTag:P4> or <FTTag:P7>)
GO:0009001; Molecular function:serine O-acetyltransferase activity
GO:0006535; Biological process:cysteine biosynthetic process from serine
end case
GO:0005737; Cellular component:cytoplasm

Cross-references [?]

Pfam PF04204; HTS; 1;
PIRSF PIRSF000450; H_ser_succinyltr; 1;
NCBIfam TIGR01001; MetA; 1;

Features [?]

From: METAA_BACC1 (Q72X44)
Key From To Description Tag Condition FTGroup
ACT_SITE 142 142 /note="Acyl-thioester intermediate" C 1
ACT_SITE 235 235 /note="Proton acceptor" H 1
ACT_SITE 237 237 E
BINDING 163 163 /ligand="substrate" K 1
BINDING 192 192 /ligand="substrate" S
BINDING 249 249 /ligand="substrate" R 1
SITE 109 109 /note="Important for acyl-CoA specificity" P4 [NE]
SITE 111 111 /note="Important for acyl-CoA specificity" P5a [GA]
SITE 111 111 /note="Important for acyl-CoA specificity" P5b [ED]
SITE 143 143 /note="Important for acyl-CoA specificity" P7 L
SITE 192 192 /note="Important for substrate specificity" P10a A
SITE 192 192 /note="Important for substrate specificity" P10b S

Additional information [?]

Size range 250-385 amino acids
Related rules None
Fusion Nter: None Cter: None
Comments Classification as HST, HAT or SAT was done according to Bastard et al. (2017) (PubMed:28581482).



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