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HAMAP rule MF_00339

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General rule information [?]

Accession MF_00339
Dates 1-JUN-2001 (Created)
1-JUN-2023 (Last updated, Version 45)
Name Phosphofructokinase_I_B1
Scope(s) Bacteria
Template(s) P0A796 (PFKA_ECOLI); P00512 (PFKA_GEOSE); P0DOB6 (PFKA1_LACLL); Q9WY52 (PFKA_THEMA); P21777 (PFKA1_THET8); [ Recover all ]
Triggered by HAMAP; MF_00339 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier PFKA
Protein name RecName: Full=ATP-dependent 6-phosphofructokinase;
                 Short=ATP-PFK;
                 Short=Phosphofructokinase;
                 EC=2.7.1.11;
AltName: Full=Phosphohexokinase;
Gene name Name=pfkA;

Comments [?]

FUNCTIONCatalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.
CATALYTIC ACTIVITY Reaction=ATP + beta-D-fructose 6-phosphate = ADP + beta-D-fructose 1,6- bisphosphate + H(+); Xref=Rhea:RHEA:16109, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:32966, ChEBI:CHEBI:57634, ChEBI:CHEBI:456216; EC=2.7.1.11;
COFACTOR Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
ACTIVITY REGULATIONAllosterically activated by ADP and other diphosphonucleosides, and allosterically inhibited by phosphoenolpyruvate.
PATHWAYCarbohydrate degradation; glycolysis; D-glyceraldehyde 3- phosphate and glycerone phosphate from D-glucose: step 3/4.
SUBUNITHomotetramer.
SUBCELLULAR LOCATIONCytoplasm.
SIMILARITYBelongs to the phosphofructokinase type A (PFKA) family. ATP-dependent PFK group I subfamily. Prokaryotic clade 'B1' sub- subfamily.

Keywords [?]


Gene Ontology [?]

GO:0003872; Molecular function:6-phosphofructokinase activity
GO:0006096; Biological process:glycolytic process
GO:0005737; Cellular component:cytoplasm

Cross-references [?]

Pfam PF00365; PFK; 1;
PIRSF PIRSF000532; ATP_PFK_prok; 1;
PRINTS PR00476; PHFRCTKINASE; 1;
NCBIfam TIGR02482; PFKA_ATP; 1;
PROSITE PS00433; PHOSPHOFRUCTOKINASE; 1;

Features [?]

From: PFKA_ECOLI (P0A796)
Key From To Description Tag Condition FTGroup
BINDING 73 74 /ligand="ATP"
/ligand_id="ChEBI:CHEBI:30616"
R-x
BINDING 103 106 /ligand="ATP"
/ligand_id="ChEBI:CHEBI:30616"
G-[DEN]-G-[ST]
BINDING 22 26 /ligand="ADP"
/ligand_id="ChEBI:CHEBI:456216"
/ligand_note="allosteric activator; ligand shared between dimeric partners"
R-x(3)-[RK]
BINDING 55 60 /ligand="ADP"
/ligand_id="ChEBI:CHEBI:456216"
/ligand_note="allosteric activator; ligand shared between dimeric partners"
R-Y-x(2)-S-[DE]
BINDING 126 128 /ligand="substrate"
/ligand_note="ligand shared between dimeric partners"
/note="in other chain"
[TS]-x-D
BINDING 170 172 /ligand="substrate"
/ligand_note="ligand shared between dimeric partners"
/note="in other chain"
M-G-[RHN]
BINDING 186 188 /ligand="ADP"
/ligand_id="ChEBI:CHEBI:456216"
/ligand_note="allosteric activator; ligand shared between dimeric partners"
/note="in other chain"
G-x-[ED]
BINDING 214 216 /ligand="ADP"
/ligand_id="ChEBI:CHEBI:456216"
/ligand_note="allosteric activator; ligand shared between dimeric partners"
/note="in other chain"
K-x(2)
BINDING 250 253 /ligand="substrate"
/ligand_note="ligand shared between dimeric partners"
/note="in other chain"
[HY]-x(2)-R
ACT_SITE 128 128 /note="Proton acceptor" D
BINDING 104 104 /ligand="Mg(2+)"
/ligand_id="ChEBI:CHEBI:18420"
/ligand_note="catalytic"
[DEN]
BINDING 12 12 /ligand="ATP"
/ligand_id="ChEBI:CHEBI:30616"
G
BINDING 155 155 /ligand="ADP"
/ligand_id="ChEBI:CHEBI:456216"
/ligand_note="allosteric activator; ligand shared between dimeric partners"
/note="in other chain"
[RK]
BINDING 163 163 /ligand="substrate"
/ligand_note="ligand shared between dimeric partners"
R
BINDING 212 212 /ligand="ADP"
/ligand_id="ChEBI:CHEBI:456216"
/ligand_note="allosteric activator; ligand shared between dimeric partners"
/note="in other chain"
[RK]
BINDING 223 223 /ligand="substrate"
/ligand_note="ligand shared between dimeric partners"
/note="in other chain"
E
BINDING 244 244 /ligand="substrate"
/ligand_note="ligand shared between dimeric partners"
[RK]

Additional information [?]

Size range 319-361 amino acids
Related rules None
Fusion Nter: None Cter: None
Comments Classification of type A phosphofructokinases into subfamilies was done according to Mueller at al.(2001) (PubMed:11673446) and Bapteste et al.(2003) (PubMed:14585511).



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