HAMAP logo

HAMAP rule MF_00463

Send feedback

General rule information [?]

Accession MF_00463
Dates 15-OCT-2001 (Created)
1-JUN-2023 (Last updated, Version 52)
Name RsxB_RnfB
Scope(s) Archaea
Bacteria
Template(s) P77223 (RSXB_ECOLI); P0CZ14 (RNFB_RHOCA); D5ARZ0 (RNFB_RHOCB); A5F2R3 (RNFB_VIBC3); H6LC27 (RNFB_ACEWD); D8GR71 (RNFB_CLOLD); Q8TSX9 (RNFB_METAC); [ Recover all ]
Triggered by HAMAP; MF_00463 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

case <OC:Escherichia> or <OC:Shigella> or <OC:Salmonella>
Identifier RSXB
Protein name RecName: Full=Ion-translocating oxidoreductase complex subunit B;
                 EC=7.-.-.-;
AltName: Full=Rsx electron transport complex subunit B;
Gene name Name=rsxB;
else
Identifier RNFB
Protein name RecName: Full=Ion-translocating oxidoreductase complex subunit B;
                 EC=7.-.-.-;
AltName: Full=Rnf electron transport complex subunit B;
Gene name Name=rnfB;
end case

Comments [?]

case <OC:Escherichia> or <OC:Shigella> or <OC:Salmonella>
FUNCTIONPart of a membrane-bound complex that couples electron transfer with translocation of ions across the membrane. Required to maintain the reduced state of SoxR.
else
FUNCTIONPart of a membrane-bound complex that couples electron transfer with translocation of ions across the membrane.
end case
case <FTGroup:1> and (<FTGroup:2> or <FTGroup:4>) and <FTGroup:3> and not <FTGroup:5>
COFACTOR Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883; Note=Binds 3 [4Fe-4S] clusters
else case <FTGroup:1> and (<FTGroup:2> or <FTGroup:4>) and <FTGroup:3> and <FTGroup:5>
COFACTOR Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883; Note=Binds 4 [4Fe-4S] clusters
else
COFACTOR Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
end case
case <OC:Escherichia> or <OC:Shigella> or <OC:Salmonella>
SUBUNITThe complex is composed of six subunits: RsxA, RsxB, RsxC, RsxD, RsxE and RsxG.
else case <OC:Bacteria>
SUBUNITThe complex is composed of six subunits: RnfA, RnfB, RnfC, RnfD, RnfE and RnfG.
else case <OC:Archaea>
SUBUNITThe Rnf complex is probably composed of eight subunits, including RnfA, RnfB, RnfC, RnfD, RnfE and RnfG.
end case
case (<OC:Pseudomonadota> or (defined <Property:Membrane> and <Property:Membrane=2>)) and not <OC:Paracoccaceae>
SUBCELLULAR LOCATIONCell inner membrane.
else case <OC:Paracoccaceae>
SUBCELLULAR LOCATIONCellular chromatophore membrane; Peripheral membrane protein; Cytoplasmic side.
else
SUBCELLULAR LOCATIONCell membrane.
end case
SIMILARITYBelongs to the 4Fe4S bacterial-type ferredoxin family. RnfB subfamily.

Keywords [?]

4Fe-4S
case (<OC:Pseudomonadota> or (defined <Property:Membrane> and <Property:Membrane=2>)) and not <OC:Paracoccaceae>
Cell inner membrane
end case
case not <OC:Paracoccaceae>
Cell membrane
end case
Electron transport
Iron
Iron-sulfur
Membrane
Metal-binding
case <OC:Paracoccaceae>
Nitrogen fixation
end case
Repeat
<a href="https://www.uniprot.org/keywords/KW-1278">Translocase</a>
Transport

Gene Ontology [?]

GO:0051539; Molecular function:4 iron, 4 sulfur cluster binding
GO:0022900; Biological process:electron transport chain
case <OCellular component:Paracoccaceae>
GO:0009399; Biological process:nitrogen fixation
end case
case not <OCellular component:Paracoccaceae>
GO:0005886; Cellular component:plasma membrane
end case

Cross-references [?]

Pfam PF00037; Fer4; 2;
Pfam PF04060; FeS; 1;
PRINTS PR00353; 4FE4SFRDOXIN; 1;
NCBIfam TIGR01944; rnfB; 1;
PROSITE PS51656; 4FE4S; 1;
PROSITE PS00198; 4FE4S_FER_1; 2;
PROSITE PS51379; 4FE4S_FER_2; 2;

Features [?]

From: RNFB_RHOCA (P0CZ14)
Key From To Description Tag Condition FTGroup
REGION Nter 23 /note="Hydrophobic"
BINDING 46 46 /ligand="[4Fe-4S] cluster"
/ligand_id="ChEBI:CHEBI:49883"
/ligand_label="1"
C 1
BINDING 49 49 /ligand="[4Fe-4S] cluster"
/ligand_id="ChEBI:CHEBI:49883"
/ligand_label="1"
C 1
BINDING 54 54 /ligand="[4Fe-4S] cluster"
/ligand_id="ChEBI:CHEBI:49883"
/ligand_label="1"
C 1
BINDING 71 71 /ligand="[4Fe-4S] cluster"
/ligand_id="ChEBI:CHEBI:49883"
/ligand_label="1"
C 1
BINDING 112 112 /ligand="[4Fe-4S] cluster"
/ligand_id="ChEBI:CHEBI:49883"
/ligand_label="2"
C 2
BINDING 115 115 /ligand="[4Fe-4S] cluster"
/ligand_id="ChEBI:CHEBI:49883"
/ligand_label="2"
C 2
BINDING 118 118 /ligand="[4Fe-4S] cluster"
/ligand_id="ChEBI:CHEBI:49883"
/ligand_label="2"
C 2
BINDING 122 122 /ligand="[4Fe-4S] cluster"
/ligand_id="ChEBI:CHEBI:49883"
/ligand_label="3"
C 3
BINDING 142 142 /ligand="[4Fe-4S] cluster"
/ligand_id="ChEBI:CHEBI:49883"
/ligand_label="3"
C 3
BINDING 145 145 /ligand="[4Fe-4S] cluster"
/ligand_id="ChEBI:CHEBI:49883"
/ligand_label="3"
C 3
BINDING 148 148 /ligand="[4Fe-4S] cluster"
/ligand_id="ChEBI:CHEBI:49883"
/ligand_label="3"
C 3
BINDING 152 152 /ligand="[4Fe-4S] cluster"
/ligand_id="ChEBI:CHEBI:49883"
/ligand_label="2"
C 2
From: RNFB_ACEWD (H6LC27)
Key From To Description Tag Condition FTGroup
BINDING 138 138 /ligand="[4Fe-4S] cluster"
/ligand_id="ChEBI:CHEBI:49883"
/ligand_label="2"
C 4
BINDING 142 142 /ligand="[4Fe-4S] cluster"
/ligand_id="ChEBI:CHEBI:49883"
/ligand_label="2"
C 4
BINDING 148 148 /ligand="[4Fe-4S] cluster"
/ligand_id="ChEBI:CHEBI:49883"
/ligand_label="2"
C 4
BINDING 182 182 /ligand="[4Fe-4S] cluster"
/ligand_id="ChEBI:CHEBI:49883"
/ligand_label="2"
C 4
BINDING 217 217 /ligand="[4Fe-4S] cluster"
/ligand_id="ChEBI:CHEBI:49883"
/ligand_label="4"
C 5
BINDING 220 220 /ligand="[4Fe-4S] cluster"
/ligand_id="ChEBI:CHEBI:49883"
/ligand_label="4"
C 5
BINDING 223 223 /ligand="[4Fe-4S] cluster"
/ligand_id="ChEBI:CHEBI:49883"
/ligand_label="4"
C 5
BINDING 227 227 /ligand="[4Fe-4S] cluster"
/ligand_id="ChEBI:CHEBI:49883"
/ligand_label="4"
C 5

Additional information [?]

Size range 160-400 amino acids
Related rules None
Fusion Nter: None Cter: None
Comments According to PubMed:9154934, this protein is a peripheral membrane protein, and the N-terminal hydrophobic stretch is not a membrane anchor. According to PubMed:25831459, this protein is a transmembrane protein.



View rule in raw text format (no links)