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HAMAP rule MF_00743

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General rule information [?]

Accession MF_00743
Dates 4-DEC-2003 (Created)
28-NOV-2023 (Last updated, Version 32)
Name FumaraseC
Scope(s) Bacteria
Archaea
Template(s) P05042 (FUMC_ECOLI); P9WN93 (FUMC_MYCTU); P39461 (FUMC_SACS2); [ Recover all ]
Triggered by HAMAP; MF_00743 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier FUMC
Protein name RecName: Full=Fumarate hydratase class II;
                 Short=Fumarase C;
                 EC=4.2.1.2;
AltName: Full=Aerobic fumarase;
AltName: Full=Iron-independent fumarase;
Gene name Name=fumC;

Comments [?]

FUNCTIONInvolved in the TCA cycle. Catalyzes the stereospecific interconversion of fumarate to L-malate.
CATALYTIC ACTIVITY Reaction=(S)-malate = fumarate + H2O; Xref=Rhea:RHEA:12460, ChEBI:CHEBI:15377, ChEBI:CHEBI:15589, ChEBI:CHEBI:29806; EC=4.2.1.2;
PATHWAYCarbohydrate metabolism; tricarboxylic acid cycle; (S)-malate from fumarate: step 1/1.
SUBUNITHomotetramer.
SUBCELLULAR LOCATIONCytoplasm.
MISCELLANEOUSThere are 2 substrate-binding sites: the catalytic A site, and the non-catalytic B site that may play a role in the transfer of substrate or product between the active site and the solvent. Alternatively, the B site may bind allosteric effectors.
SIMILARITYBelongs to the class-II fumarase/aspartase family. Fumarase subfamily.

Keywords [?]


Gene Ontology [?]

GO:0004333; Molecular function:fumarate hydratase activity
GO:0006099; Biological process:tricarboxylic acid cycle
GO:0005737; Cellular component:cytoplasm

Cross-references [?]

Pfam PF10415; FumaraseC_C; 1;
Pfam PF00206; Lyase_1; 1;
PRINTS PR00149; FUMRATELYASE; 1;
NCBIfam TIGR00979; FumC_II; 1;
PROSITE PS00163; FUMARATE_LYASES; 1;

Features [?]

From: FUMC_MYCTU (P9WN93)
Key From To Description Tag Condition FTGroup
BINDING 104 106 /ligand="substrate" S-x-T
BINDING 128 131 /ligand="substrate"
/note="in site B"
[HN]-x-N-D
BINDING 138 140 /ligand="substrate" S-[ST]-N
BINDING 324 326 /ligand="substrate" K-x-N
ACT_SITE 187 187 /note="Proton donor/acceptor" H
ACT_SITE 318 318 S
BINDING 186 186 /ligand="substrate" T
BINDING 319 319 /ligand="substrate" S
SITE 331 331 /note="Important for catalytic activity" E

Additional information [?]

Size range 438-484 amino acids
Related rules None
Fusion Nter: None Cter: None



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