HAMAP rule MF_01263

General rule information [?]

Accession	MF_01263
Dates	29-JUL-2005 (Created) 17-MAY-2023 (Last updated, Version 21)
Name	CCA_bact_type3
Scope(s)	Bacteria Bacillota
Template(s)	Q7SIB1 (CCA_GEOSE); P42977 (CCA_BACSU); [ Recover all ]
Triggered by	HAMAP; MF_01263 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier	CCA
Protein name	RecName: Full=CCA-adding enzyme; EC=2.7.7.72; AltName: Full=CCA tRNA nucleotidyltransferase; AltName: Full=tRNA CCA-pyrophosphorylase; AltName: Full=tRNA adenylyl-/cytidylyl- transferase; AltName: Full=tRNA nucleotidyltransferase; AltName: Full=tRNA-NT;
Gene name	Name=cca;

Comments [?]

FUNCTION	Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing inorganic pyrophosphate. tRNA 3'-terminal CCA addition is required both for tRNA processing and repair. Also involved in tRNA surveillance by mediating tandem CCA addition to generate a CCACCA at the 3' terminus of unstable tRNAs. While stable tRNAs receive only 3'-terminal CCA, unstable tRNAs are marked with CCACCA and rapidly degraded.
CATALYTIC ACTIVITY	Reaction=a tRNA precursor + ATP + 2 CTP = a tRNA with a 3' CCA end + 3 diphosphate; Xref=Rhea:RHEA:14433, Rhea:RHEA-COMP:10465, Rhea:RHEA- COMP:10468, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:37563, ChEBI:CHEBI:74896, ChEBI:CHEBI:83071; EC=2.7.7.72;
CATALYTIC ACTIVITY	Reaction=a tRNA with a 3' CCA end + ATP + 2 CTP = a tRNA with a 3' CCACCA end + 3 diphosphate; Xref=Rhea:RHEA:76235, Rhea:RHEA- COMP:10468, Rhea:RHEA-COMP:18655, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:37563, ChEBI:CHEBI:83071, ChEBI:CHEBI:195187; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:76236;
COFACTOR	Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
SUBUNIT	Homodimer.
MISCELLANEOUS	A single active site specifically recognizes both ATP and CTP and is responsible for their addition.
SIMILARITY	Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 3 subfamily.

Keywords [?]

Nucleotidyltransferase

Gene Ontology [?]

GO:0000049; Molecular function:tRNA binding

GO:0000166; Molecular function:nucleotide binding

GO:0000287; Molecular function:magnesium ion binding

GO:0004810; Molecular function:CCA tRNA nucleotidyltransferase activity

GO:0005524; Molecular function:ATP binding

GO:0001680; Biological process:tRNA 3'-terminal CCA addition

Cross-references [?]

Pfam	PF01743; PolyA_pol; 1;

Features [?]

From: CCA_GEOSE (Q7SIB1)

Key

From

Description

Tag

Condition

FTGroup

BINDING

/ligand="Mg(2+)"
/ligand_id="ChEBI:CHEBI:18420"

BINDING

/ligand="Mg(2+)"
/ligand_id="ChEBI:CHEBI:18420"

BINDING