HAMAP rule MF_01447
General rule information
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Accession | MF_01447 |
Dates | 13-AUG-2007 (Created)
1-JUN-2023 (Last updated, Version 23) |
Name | Kae1_Bud32_arch |
Scope(s) |
Archaea Euryarchaeota |
Template(s) | Q58530 (KAE1B_METJA); P36132 (KAE1_YEAST); [ Recover all ] |
Triggered by |
HAMAP; MF_01447 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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Identifier | KAE1B |
Protein name | RecName: Full=Probable bifunctional tRNA threonylcarbamoyladenosine biosynthesis protein; Includes: RecName: Full=tRNA N6-adenosine threonylcarbamoyltransferase; EC=2.3.1.234; AltName: Full=tRNA threonylcarbamoyladenosine biosynthesis protein Kae1; AltName: Full=t(6)A37 threonylcarbamoyladenosine biosynthesis protein Kae1; Includes: RecName: Full=Serine/threonine-protein kinase Bud32; EC=2.7.11.1; |
Comments
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FUNCTION | Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. Is a component of the KEOPS complex that is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37. The Kae1 domain likely plays a direct catalytic role in this reaction. The Bud32 domain probably displays kinase activity that regulates Kae1 function. |
CATALYTIC ACTIVITY | Reaction=adenosine(37) in tRNA + L-threonylcarbamoyladenylate = AMP + H(+) + N(6)-L-threonylcarbamoyladenosine(37) in tRNA; Xref=Rhea:RHEA:37059, Rhea:RHEA-COMP:10162, Rhea:RHEA-COMP:10163, ChEBI:CHEBI:15378, ChEBI:CHEBI:73682, ChEBI:CHEBI:74411, ChEBI:CHEBI:74418, ChEBI:CHEBI:456215; EC=2.3.1.234; |
CATALYTIC ACTIVITY | Reaction=ATP + L-seryl-[protein] = ADP + H(+) + O-phospho-L-seryl- [protein]; Xref=Rhea:RHEA:17989, Rhea:RHEA-COMP:9863, Rhea:RHEA- COMP:11604, ChEBI:CHEBI:15378, ChEBI:CHEBI:29999, ChEBI:CHEBI:30616, ChEBI:CHEBI:83421, ChEBI:CHEBI:456216; EC=2.7.11.1; |
CATALYTIC ACTIVITY | Reaction=ATP + L-threonyl-[protein] = ADP + H(+) + O-phospho-L- threonyl-[protein]; Xref=Rhea:RHEA:46608, Rhea:RHEA-COMP:11060, Rhea:RHEA-COMP:11605, ChEBI:CHEBI:15378, ChEBI:CHEBI:30013, ChEBI:CHEBI:30616, ChEBI:CHEBI:61977, ChEBI:CHEBI:456216; EC=2.7.11.1; |
COFACTOR | Name=Fe(2+); Xref=ChEBI:CHEBI:29033; Note=Binds 1 Fe(2+) ion per subunit.; |
SUBUNIT | Component of the KEOPS complex that consists of Kae1, Bud32, Cgi121 and Pcc1; the whole complex dimerizes. |
SUBCELLULAR LOCATION | Cytoplasm. |
SIMILARITY | In the N-terminal section; belongs to the KAE1 / TsaD family. |
SIMILARITY | In the C-terminal section; belongs to the protein kinase superfamily. Tyr protein kinase family. BUD32 subfamily. |
Keywords
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Acyltransferase |
ATP-binding |
Cytoplasm |
Kinase |
Nucleotide-binding |
Serine/threonine-protein kinase |
Transferase |
tRNA processing |
Iron |
Metal-binding |
Multifunctional enzyme |
Gene Ontology
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GO:0005506; Molecular function:iron ion binding |
GO:0005524; Molecular function:ATP binding |
GO:0004712; Molecular function:protein serine/threonine/tyrosine kinase activity |
GO:0016747; Molecular function:acyltransferase activity, transferring groups other than amino-acyl groups |
GO:0002949; Biological process:tRNA threonylcarbamoyladenosine modification |
GO:0005737; Cellular component:cytoplasm |
Cross-references
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PROSITE | PS01016; GLYCOPROTEASE; 1; |
PROSITE | PS00107; PROTEIN_KINASE_ATP; 1; |
PROSITE | PS50011; PROTEIN_KINASE_DOM; 1; |
PROSITE | PS00109; PROTEIN_KINASE_TYR; 1; |
Pfam | PF00814; Peptidase_M22; 1; |
Pfam | PF00069; Pkinase; 1; |
Pfam | PF01163; RIO1; 1; |
PRINTS | PR00789; OSIALOPTASE; 1; |
NCBIfam | TIGR03724; Arch_bud32; 1; |
NCBIfam | TIGR03722; Arch_KAE1; 1; |
NCBIfam | TIGR00329; Gcp_kae1; 1; |
PIRSF | PIRSF036401; Gcp_STYKS; 1; |
Features
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From: KAE1B_METJA (Q58530) | ||||||||||||
Key | From | To | Description | Tag | Condition | FTGroup | ||||||
DOMAIN | 333 | Cter | /note="Protein kinase" | |||||||||
BINDING | 339 | 347 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616" |
|||||||||
REGION | Nter | 323 | /note="Kae1" | |||||||||
ACT_SITE | 451 | 451 | /note="Proton acceptor; for kinase activity" | D | ||||||||
BINDING | 127 | 131 | /ligand="L-threonylcarbamoyladenylate" /ligand_id="ChEBI:CHEBI:73682" |
x-x-S-G-[GA] | ||||||||
BINDING | 106 | 106 | /ligand="Fe cation" /ligand_id="ChEBI:CHEBI:24875" |
H | ||||||||
BINDING | 110 | 110 | /ligand="Fe cation" /ligand_id="ChEBI:CHEBI:24875" |
H | ||||||||
BINDING | 127 | 127 | /ligand="Fe cation" /ligand_id="ChEBI:CHEBI:24875" |
Y | ||||||||
BINDING | 284 | 284 | /ligand="Fe cation" /ligand_id="ChEBI:CHEBI:24875" |
D | ||||||||
BINDING | 159 | 159 | /ligand="L-threonylcarbamoyladenylate" /ligand_id="ChEBI:CHEBI:73682" |
D | ||||||||
BINDING | 172 | 172 | /ligand="L-threonylcarbamoyladenylate" /ligand_id="ChEBI:CHEBI:73682" |
G | ||||||||
BINDING | 176 | 176 | /ligand="L-threonylcarbamoyladenylate" /ligand_id="ChEBI:CHEBI:73682" |
E | ||||||||
BINDING | 256 | 256 | /ligand="L-threonylcarbamoyladenylate" /ligand_id="ChEBI:CHEBI:73682" |
N | ||||||||
BINDING | 360 | 360 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616" |
K |
Additional information
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Size range | 520-548 amino acids |
Related rules |
MF_01446 |
Fusion | Nter: None Cter: None |