HAMAP rule MF_01974

General rule information [?]

Accession	MF_01974
Dates	31-MAY-2013 (Created) 1-JUN-2023 (Last updated, Version 11)
Name	MetAP_1
Scope(s)	Bacteria
Template(s)	P0AE18 (MAP1_ECOLI); P9WK19 (MAP12_MYCTU); Q9ZCD3 (MAP1_RICPR); [ Recover all ]
Triggered by	case c? <OC:Bacteria> HAMAP; MF_01974 (Get profile general information and statistics) end case

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier	MAP1
Protein name	RecName: Full=Methionine aminopeptidase; Short=MAP; Short=MetAP; EC=3.4.11.18; AltName: Full=Peptidase M;
Gene name	Name=map;

Comments [?]

FUNCTION	Removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val). Requires deformylation of the N(alpha)-formylated initiator methionine before it can be hydrolyzed.
CATALYTIC ACTIVITY	Reaction=Release of N-terminal amino acids, preferentially methionine, from peptides and arylamides.; EC=3.4.11.18;
COFACTOR	Name=Co(2+); Xref=ChEBI:CHEBI:48828; Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Name=Fe(2+); Xref=ChEBI:CHEBI:29033; Note=Binds 2 divalent metal cations per subunit. Has a high-affinity and a low affinity metal-binding site. The true nature of the physiological cofactor is under debate. The enzyme is active with cobalt, zinc, manganese or divalent iron ions. Most likely, methionine aminopeptidases function as mononuclear Fe(2+)-metalloproteases under physiological conditions, and the catalytically relevant metal-binding site has been assigned to the histidine-containing high-affinity site.;
SUBUNIT	Monomer.
SIMILARITY	Belongs to the peptidase M24A family. Methionine aminopeptidase type 1 subfamily.

Keywords [?]

Gene Ontology [?]

GO:0046872; Molecular function:metal ion binding

GO:0070006; Molecular function:metalloaminopeptidase activity

GO:0004239; Molecular function:initiator methionyl aminopeptidase activity

Cross-references [?]

Features [?]

From: MAP1_ECOLI (P0AE18)
Key	From	To	Description	Tag	Condition	FTGroup
BINDING	97	97	/ligand="a divalent metal cation" /ligand_id="ChEBI:CHEBI:60240" /ligand_label="1"		D	1
BINDING	108	108	/ligand="a divalent metal cation" /ligand_id="ChEBI:CHEBI:60240" /ligand_label="1"		D	1
BINDING	108	108	/ligand="a divalent metal cation" /ligand_id="ChEBI:CHEBI:60240" /ligand_label="2" /ligand_note="catalytic"		D	1
BINDING	171	171	/ligand="a divalent metal cation" /ligand_id="ChEBI:CHEBI:60240" /ligand_label="2" /ligand_note="catalytic"		H	1
BINDING	204	204	/ligand="a divalent metal cation" /ligand_id="ChEBI:CHEBI:60240" /ligand_label="2" /ligand_note="catalytic"		E	1
BINDING	235	235	/ligand="a divalent metal cation" /ligand_id="ChEBI:CHEBI:60240" /ligand_label="1"		E	1
BINDING	235	235	/ligand="a divalent metal cation" /ligand_id="ChEBI:CHEBI:60240" /ligand_label="2" /ligand_note="catalytic"		E	1
BINDING	79	79	/ligand="substrate"		H
BINDING	178	178	/ligand="substrate"		H

Additional information [?]