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HAMAP rule MF_03163

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General rule information [?]

Accession MF_03163
Dates 8-JAN-2013 (Created)
7-FEB-2023 (Last updated, Version 11)
Name RNA_methyltr_E_SPB1
Scope(s) Eukaryota
Template(s) P25582 (SPB1_YEAST); Q8IY81 (SPB1_HUMAN); [ Recover all ]
Triggered by
case c? <OC:Eukaryota>
HAMAP; MF_03163 (Get profile general information and statistics)
end case

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier SPB1
case <OC:Fungi>
Protein name RecName: Full=AdoMet-dependent rRNA methyltransferase ;
                 EC=2.1.1.-;
AltName: Full=2'-O-ribose RNA methyltransferase;
AltName: Full=S-adenosyl-L-methionine-dependent methyltransferase;
Gene name Name=SPB1;
else case <OC:Vertebrata>
Protein name RecName: Full=pre-rRNA processing protein FTSJ3;
                 EC=2.1.1.-;
AltName: Full=2'-O-ribose RNA methyltransferase SPB1 homolog;
AltName: Full=Protein ftsJ homolog 3;
AltName: Full=Putative rRNA methyltransferase 3;
Gene name Name=FTSJ3;
else
Protein name RecName: Full=Putative rRNA methyltransferase;
                 EC=2.1.1.-;
AltName: Full=2'-O-ribose RNA methyltransferase SPB1 homolog;
end case

Comments [?]

case <OC:Fungi>
FUNCTIONRequired for proper assembly of pre-ribosomal particles during the biogenesis of the 60S ribosomal subunit.
CATALYTIC ACTIVITY Reaction=a ribonucleotide in rRNA + S-adenosyl-L-methionine = a 2'-O- methylribonucleotide in rRNA + H(+) + S-adenosyl-L-homocysteine; Xref=Rhea:RHEA:48628, Rhea:RHEA-COMP:12164, Rhea:RHEA-COMP:12165, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789, ChEBI:CHEBI:90675, ChEBI:CHEBI:90676;
SUBUNITComponent of the nucleolar and nucleoplasmic pre-60S ribosomal particle.
else case <OC:Vertebrata>
FUNCTIONProbable methyltransferase involved in the processing of the 34S pre-rRNA to 18S rRNA and in 40S ribosomal subunit formation.
CATALYTIC ACTIVITY Reaction=a ribonucleotide in rRNA + S-adenosyl-L-methionine = a 2'-O- methylribonucleotide in rRNA + H(+) + S-adenosyl-L-homocysteine; Xref=Rhea:RHEA:48628, Rhea:RHEA-COMP:12164, Rhea:RHEA-COMP:12165, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789, ChEBI:CHEBI:90675, ChEBI:CHEBI:90676;
SUBUNITInteracts with NIP7.
else
FUNCTIONProbable methyltransferase involved in the maturation of rRNA and in the biogenesis of ribosomal subunits.
CATALYTIC ACTIVITY Reaction=a ribonucleotide in rRNA + S-adenosyl-L-methionine = a 2'-O- methylribonucleotide in rRNA + H(+) + S-adenosyl-L-homocysteine; Xref=Rhea:RHEA:48628, Rhea:RHEA-COMP:12164, Rhea:RHEA-COMP:12165, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789, ChEBI:CHEBI:90675, ChEBI:CHEBI:90676;
end case
SUBCELLULAR LOCATIONNucleus, nucleolus.
SIMILARITYBelongs to the class I-like SAM-binding methyltransferase superfamily. RNA methyltransferase RlmE family. SPB1 subfamily.

Keywords [?]


Gene Ontology [?]

GO:0005730; Cellular component:nucleolus
case <OCellular component:Fungi>
GO:0030687; Cellular component:preribosome, large subunit precursor
GO:0070039; Molecular function:rRNA (guanosine-2'-O-)-methyltransferase activity
GO:0008650; Molecular function:rRNA (uridine-2'-O-)-methyltransferase activity
else case <OCellular component:Vertebrata>
GO:0030688; Cellular component:preribosome, small subunit precursor
GO:0008649; Molecular function:rRNA methyltransferase activity
else; https://www.ebi.ac.uk/QuickGO/term/else
GO:0008649; Molecular function:rRNA methyltransferase activity
end case
GO:0031167; Biological process:rRNA methylation

Cross-references [?]

Pfam PF11861; DUF3381; 1;
Pfam PF01728; FtsJ; 1;
Pfam PF07780; Spb1_C; 1;
Pfam PF02520; DUF148; 1;
PROSITE PS50312; ASP_RICH; 0-unlimited;
PROSITE PS50313; GLU_RICH; 0-unlimited;
PROSITE PS50318; LYS_RICH; 0-unlimited;
General Coiled_coil; -; 0-unlimited;

Features [?]

From: SPB1_YEAST (P25582)
Key From To Description Tag Condition FTGroup
ACT_SITE 159 159 /note="Proton acceptor" K
BINDING 58 58 /ligand="S-adenosyl-L-methionine"
/ligand_id="ChEBI:CHEBI:59789"
G 1
BINDING 60 60 /ligand="S-adenosyl-L-methionine"
/ligand_id="ChEBI:CHEBI:59789"
[WF] 1
BINDING 78 78 /ligand="S-adenosyl-L-methionine"
/ligand_id="ChEBI:CHEBI:59789"
D 1
BINDING 94 94 /ligand="S-adenosyl-L-methionine"
/ligand_id="ChEBI:CHEBI:59789"
[DN] 1
BINDING 119 119 /ligand="S-adenosyl-L-methionine"
/ligand_id="ChEBI:CHEBI:59789"
D 1

Additional information [?]

Size range 736-1019 amino acids
Related rules MF_01547
MF_03162
Fusion Nter: None Cter: None



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