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HAMAP rule MF_04136

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General rule information [?]

Accession MF_04136
Dates 9-APR-2019 (Created)
9-AUG-2022 (Last updated, Version 6)
Name SAR_ENDOLYSIN
Scope(s) Viruses
Template(s) Q7Y2C0 (ENLYS_BPKMV); P27359 (ENLYS_BPP21); Q37875 (ENLYS_BPP1); [ Recover all ]
Triggered by HAMAP; MF_04136 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier ENLYS
Protein name RecName: Full=SAR-endolysin;
                 EC=3.2.1.17;
AltName: Full=Endolysin;
AltName: Full=Lysis protein;
AltName: Full=Lysozyme;
AltName: Full=Muramidase;

Comments [?]

case <OC:Muvirus>
FUNCTIONSignal-arrest-release (SAR) endolysin with lysozyme activity that degrades host peptidoglycans and participates with the releasin and spanin proteins in the sequential events which lead to programmed host cell lysis releasing the mature viral particles. The SAR-endolysin is released by the releasin protein into the periplasm, where it breaks down the peptidoglycan layer.
else
FUNCTIONSignal-arrest-release (SAR) endolysin with lysozyme activity that degrades host peptidoglycans and participates with the pinholin and spanin proteins in the sequential events which lead to programmed host cell lysis releasing the mature viral particles. Once the pinholin has permeabilized the host cell membrane, the SAR-endolysin is released into the periplasm where it breaks down the peptidoglycan layer.
end case
CATALYTIC ACTIVITY Reaction=Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins.; EC=3.2.1.17;
case <AnyFeature:Transmembrane<2>
SUBCELLULAR LOCATIONHost cell inner membrane; Single-pass type II membrane protein; Periplasmic side. Note=Secreted as a signal-anchored, membrane-tethered, inactive endolysin which is subsequently refolded, activated and released by membrane depolarization driven by the pinholin.
DOMAINThe signal-anchor, which may also be an uncleaved signal sequence tethers the SAR-endolysin to the membrane until the latter is depolarized by the holin, resulting in the escape of SAR-endolysin from the membrane.
end case
case <FTGroup:1>
PTMAll the periplasmic cyteines of the inactive, membrane-associated endolysin are involved in disulfide bond. In the active soluble form, disulfide bonds are isomerized and only the catalytic cysteine remains free.
end case
case not <FTTag:Act_site> and not <FTTag:Act_site_Cys>
CAUTIONLacks the conserved Asp active site.
end case
SIMILARITYBelongs to the glycosyl hydrolase 24 family.

Keywords [?]

Antimicrobial
Bacteriolytic enzyme
Cytolysis
Glycosidase
Host cell inner membrane
Host cell lysis by virus
Host cell membrane
Host membrane
Hydrolase
Membrane
Signal-anchor
Transmembrane
Transmembrane helix
Viral release from host cell
case <FTGroup:1>
Disulfide bond
end case

Gene Ontology [?]

GO:0003796; Molecular function:lysozyme activity
GO:0044659; Biological process:viral release from host cell by cytolysis
GO:0009253; Biological process:peptidoglycan catabolic process

Cross-references [?]

Pfam PF00959; Phage_lysozyme; 1;
General Transmembrane; -; 1;

Features [?]

From: ENLYS_BPKMV (Q7Y2C0)
Key From To Description Tag Condition FTGroup
case not <AnyFeature:Transmembrane>
TRANSMEM 1 19 /note="Helical; Signal-anchor for type II membrane protein"
end case
ACT_SITE 21 21 /note="Proton donor/acceptor" E
ACT_SITE 30 30 /note="Proton donor/acceptor" Act_site D
From: ENLYS_BPP1 (Q37875)
Key From To Description Tag Condition FTGroup
ACT_SITE 51 51 /note="Proton donor/acceptor" Act_site_Cys C
DISULFID 13 44 /note="In the active soluble endolysin; alternate" C-x*-C 1
DISULFID 44 51 /note="In the inactive membrane-associated endolysin; alternate" C-x*-C 1

Additional information [?]

Size range 140-250 amino acids
Related rules MF_04110
Fusion Nter: None Cter: None



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