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Annotation rule MF_00049
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General rule information [?]

Accession MF_00049
Dates 1-JUN-2001 (Created)
20-NOV-2019 (Last updated, Version 33)
Name Leu_tRNA_synth
Scope
Bacteria
Archaea
Template P07813 (SYL_ECOLI)
case <OC:Bacteria>
end case

case <OC:Archaea>
end case


Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
SYL
Protein name
RecName: Full=Leucine--tRNA ligase;
EC 6.1.1.4;
AltName: Full=Leucyl-tRNA synthetase;
Short=LeuRS;
Gene name
leuS

Comments [?]

Catalytic activity RHEA:11688: ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu)
EC 6.1.1.4
Subcellular location Cytoplasm.
Similarity Belongs to the class-I aminoacyl-tRNA synthetase family.

Keywords [?]


Gene Ontology [?]

GO:0005524; Molecular function: ATP binding.
GO:0004823; Molecular function: leucine-tRNA ligase activity.
GO:0006429; Biological process: leucyl-tRNA aminoacylation.
GO:0005737; Cellular component: cytoplasm.

Cross-references [?]

Pfam PF00133; tRNA-synt_1; 1;
PRINTS PR00985; TRNASYNTHLEU; 1;
TIGRFAMs TIGR00395; LeuS_arch; 1;
TIGR00396; LeuS_bact; 1;
PROSITE PS00178; AA_TRNA_LIGASE_I; 1;

Features [?]

case <OC:Bacteria>
From: SYL_ECOLI (P07813)
Key     From     To       Description   Tag   Condition   FTGroup
MOTIF     42     52       'HIGH' region     [SP]-[YF]-[PLVT]-[SN]-[GAS]-x(1,3)-H-[LIVMA]-G-[HS]  
MOTIF     619     623       'KMSKS' region     [KRTA]-[MLI]-[SGA]-[KS]-[SA]  
BINDING     622     622       ATP     K  
end case
case <OC:Archaea>
From: SYL_METJA (Q58050)
MOTIF     41     51       'HIGH' region     P-Y-x-[SN]-[GAS]-x(2)-H-[LIVMA]-G-[HS]  
MOTIF     633     637       'KMSKS' region        
BINDING     636     636       ATP     K  
end case

Additional information [?]

case <OC:Bacteria>
Size range 792-990 amino acids
end case
case <OC:Archaea>
Size range 910-977 amino acids
end case
Related rules None
Fusion None
Comments The AQUAE leuS is cut into two subunits. It is not shown in the alignment. There is a shorter second copy in SULTO, which is not shown in the alignment, nor are HALSA or NANEQ as they are quite divergent.