HAMAP rule MF_00049
General rule information
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| Accession | MF_00049 |
| Dates | 1-JUN-2001 (Created)
3-SEP-2024 (Last updated, Version 38) |
| Name | Leu_tRNA_synth |
| Scope(s) |
Bacteria Archaea |
| Template(s) | P07813 (SYL_ECOLI); [ Recover all ] |
| Triggered by |
case c? <OC:Bacteria>
HAMAP; MF_00049_B (Get profile general information and statistics) end case
case c? <OC:Archaea>
HAMAP; MF_00049_A (Get profile general information and statistics) end case
|
Propagated annotation
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Identifier, protein and gene names
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| Identifier | SYL |
| Protein name | RecName: Full=Leucine--tRNA ligase; EC=6.1.1.4; AltName: Full=Leucyl-tRNA synthetase; Short=LeuRS; |
| Gene name | Name=leuS; |
Comments
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| CATALYTIC ACTIVITY | Reaction=tRNA(Leu) + L-leucine + ATP = L-leucyl-tRNA(Leu) + AMP + diphosphate; Xref=Rhea:RHEA:11688, Rhea:RHEA-COMP:9613, Rhea:RHEA- COMP:9622, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57427, ChEBI:CHEBI:78442, ChEBI:CHEBI:78494, ChEBI:CHEBI:456215; EC=6.1.1.4; |
| SUBCELLULAR LOCATION | Cytoplasm. |
| SIMILARITY | Belongs to the class-I aminoacyl-tRNA synthetase family. |
Keywords
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Gene Ontology
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| GO:0005524; Molecular function:ATP binding |
| GO:0004823; Molecular function:leucine-tRNA ligase activity |
| GO:0006429; Biological process:leucyl-tRNA aminoacylation |
| GO:0005737; Cellular component:cytoplasm |
Cross-references
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| Pfam | PF00133; tRNA-synt_1; 1; |
| PRINTS | PR00985; TRNASYNTHLEU; 1; |
| NCBIfam | TIGR00395; LeuS_arch; 1; |
| NCBIfam | TIGR00396; LeuS_bact; 1; |
| PROSITE | PS00178; AA_TRNA_LIGASE_I; 1; |
Features
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| From: SYL_ECOLI (P07813) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| case <OC:Bacteria> | ||||||||||||
| MOTIF | 42 | 52 | /note="'HIGH' region" | [SP]-[YF]-[PLVT]-[SN]-[GAS]-x(1,3)-H-[LIVMA]-G-[HS] | ||||||||
| MOTIF | 619 | 623 | /note="'KMSKS' region" | [KRTA]-[MLI]-[SGA]-[KS]-[SA] | ||||||||
| BINDING | 622 | 622 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616" |
K | ||||||||
| end case | ||||||||||||
| From: SYL_METJA (Q58050) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| case <OC:Archaea> | ||||||||||||
| MOTIF | 41 | 51 | /note="'HIGH' region" | P-Y-x-[SN]-[GAS]-x(2)-H-[LIVMA]-G-[HS] | ||||||||
| MOTIF | 633 | 637 | /note="'KMSKS' region" | |||||||||
| BINDING | 636 | 636 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616" |
K | ||||||||
| end case | ||||||||||||
Additional information
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| Size range | 910-977 amino acids |
| Related rules |
None |
| Fusion | Nter: None Cter: None |
| Comments | The AQUAE leuS is cut into two subunits. It is not shown in the alignment. There is a shorter second copy in SULTO, which is not shown in the alignment, nor are HALSA or NANEQ as they are quite divergent. |