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HAMAP rule MF_00100

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General rule information [?]

Accession MF_00100
Dates 1-JUN-2001 (Created)
1-JUN-2023 (Last updated, Version 53)
Name IF_2
Scope
Bacteria
Archaea
Plastid
Templates P04766 (IF2_GEOSE); P0A705 (IF2_ECOLI); P48515 (IF2_THET8); Q9HNQ2 (IF2P_HALSA); O26359 (IF2P_METTH): [Recover all]
case <OC:Bacteria> or <OG:Chloroplast>
Triggered by
end case

case <OC:Archaea>
Triggered by
end case


Propagated annotation [?]


Identifier, protein and gene names [?]

case <OC:Bacteria>
Identifier
IF2
Protein name
RecName: Full=Translation initiation factor IF-2;
end case
case <OG:Chloroplast>
Identifier
IF2C
Protein name
RecName: Full=Translation initiation factor IF-2, chloroplastic;
end case
case <OC:Archaea>
Identifier
IF2P
Protein name
RecName: Full=Probable translation initiation factor IF-2;
end case
Gene name
infB

Comments [?]

case <OC:Bacteria>
Function One of the essential components for the initiation of protein synthesis. Protects formylmethionyl-tRNA from spontaneous hydrolysis and promotes its binding to the 30S ribosomal subunits. Also involved in the hydrolysis of GTP during the formation of the 70S ribosomal complex.
Subcellular location Cytoplasm.
end case
case <OC:Archaea>
Function Function in general translation initiation by promoting the binding of the formylmethionine-tRNA to ribosomes. Seems to function along with eIF-2.
end case
case <OG:Chloroplast>
Function One of the essential components for the initiation of protein synthesis. Protects formylmethionyl-tRNA from spontaneous hydrolysis and promotes its binding to the 30S ribosomal subunits. Also involved in the hydrolysis of GTP during the formation of the 70S ribosomal complex.
Subcellular location Plastid, chloroplast.
end case
Similarity Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. IF-2 subfamily.

Keywords [?]

case <OC:Bacteria>
Cytoplasm
end case
Initiation factor
Protein biosynthesis
GTP-binding
Nucleotide-binding

Gene Ontology [?]

GO:0003924; Molecular function: GTPase activity.
GO:0003743; Molecular function: translation initiation factor activity.
GO:0006413; Biological process: translational initiation.
case <OC:Bacteria>
GO:0005737; Cellular component: cytoplasm.
end case
case <OG:Chloroplast>
GO:0009507; Cellular component: chloroplast.
end case

Cross-references [?]

Pfam PF00009; GTP_EFTU; 1;
NCBIfam TIGR00487; IF-2; 1;
TIGR00491; AIF-2; 1;
PROSITE PS51722; G_TR_2; 1;
PS01176; IF2; 1;

Features [?]

case <OC:Bacteria> or <OG:Chloroplast>
From: IF2_ECOLI (P0A705)
Key     From     To       Description   Tag   Condition   FTGroup
BINDING     398     405       /ligand="GTP" /ligand_id="ChEBI:CHEBI:37565     G-H-V-D-H-G-K-T  
BINDING     444     448       /ligand="GTP" /ligand_id="ChEBI:CHEBI:37565     D-T-P-G-H  
BINDING     498     501       /ligand="GTP" /ligand_id="ChEBI:CHEBI:37565     [NST]-K-[LIVCMT]-D  
REGION     392     540       G-domain        
end case
case <OC:Archaea>
From: IF2P_METTH (O26359)
BINDING     12     19       /ligand="GTP" /ligand_id="ChEBI:CHEBI:37565     G-H-V-D-[HVA]-G-K-T  
BINDING     76     80       /ligand="GTP" /ligand_id="ChEBI:CHEBI:37565     D-T-P-G-H  
BINDING     130     133       /ligand="GTP" /ligand_id="ChEBI:CHEBI:37565     [NT]-K-[LIV]-D  
end case

Additional information [?]

case <OC:Bacteria>
Size range 571-1183 amino acids
end case
case <OC:Archaea>
Size range 589-620 amino acids
end case
Related rules None
Fusion None
Comments Intein in some members.


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