HAMAP rule MF_00100
General rule information
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Accession | MF_00100 |
Dates | 1-JUN-2001 (Created) 1-JUN-2023 (Last updated, Version 53) |
Name | IF_2 |
Scope | Bacteria
Archaea
Plastid |
Templates | P04766 (IF2_GEOSE); P0A705 (IF2_ECOLI); P48515 (IF2_THET8); Q9HNQ2 (IF2P_HALSA); O26359 (IF2P_METTH): [Recover all] |
case <OC:Bacteria> or <OG:Chloroplast>
Triggered by |
end case
case <OC:Archaea>
Triggered by |
end case
Propagated annotation
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Identifier, protein and gene names
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case <OC:Bacteria>
Identifier |
|
Protein name |
|
end case
case <OG:Chloroplast>
Identifier |
|
Protein name |
|
end case
case <OC:Archaea>
Identifier |
|
Protein name |
|
end case
Gene name |
|
Comments
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case <OC:Bacteria>
Function | One of the essential components for the initiation of protein synthesis. Protects formylmethionyl-tRNA from spontaneous hydrolysis and promotes its binding to the 30S ribosomal subunits. Also involved in the hydrolysis of GTP during the formation of the 70S ribosomal complex. |
Subcellular location | Cytoplasm. |
end case
case <OC:Archaea>
Function | Function in general translation initiation by promoting the binding of the formylmethionine-tRNA to ribosomes. Seems to function along with eIF-2. |
end case
case <OG:Chloroplast>
Function | One of the essential components for the initiation of protein synthesis. Protects formylmethionyl-tRNA from spontaneous hydrolysis and promotes its binding to the 30S ribosomal subunits. Also involved in the hydrolysis of GTP during the formation of the 70S ribosomal complex. |
Subcellular location | Plastid, chloroplast. |
end case
Similarity | Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. IF-2 subfamily. |
Keywords
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case <OC:Bacteria>
end case
Gene Ontology
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GO:0003924; Molecular function: GTPase activity.
GO:0003743; Molecular function: translation initiation factor activity.
GO:0006413; Biological process: translational initiation.
GO:0003743; Molecular function: translation initiation factor activity.
GO:0006413; Biological process: translational initiation.
case <OC:Bacteria>
GO:0005737; Cellular component: cytoplasm.
end case
case <OG:Chloroplast>
GO:0009507; Cellular component: chloroplast.
end case
Cross-references
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Pfam | PF00009; GTP_EFTU; 1; |
NCBIfam | TIGR00487; IF-2; 1; |
TIGR00491; AIF-2; 1; | |
PROSITE | PS51722; G_TR_2; 1; |
PS01176; IF2; 1; |
Features
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case <OC:Bacteria> or <OG:Chloroplast>
From: IF2_ECOLI (P0A705) | ||||||||||||
Key | From | To | Description | Tag | Condition | FTGroup | ||||||
BINDING | 398 | 405 | /ligand="GTP" /ligand_id="ChEBI:CHEBI:37565 | G-H-V-D-H-G-K-T | ||||||||
BINDING | 444 | 448 | /ligand="GTP" /ligand_id="ChEBI:CHEBI:37565 | D-T-P-G-H | ||||||||
BINDING | 498 | 501 | /ligand="GTP" /ligand_id="ChEBI:CHEBI:37565 | [NST]-K-[LIVCMT]-D | ||||||||
REGION | 392 | 540 | G-domain |
end case
case <OC:Archaea>
From: IF2P_METTH (O26359) | ||||||||||||
BINDING | 12 | 19 | /ligand="GTP" /ligand_id="ChEBI:CHEBI:37565 | G-H-V-D-[HVA]-G-K-T | ||||||||
BINDING | 76 | 80 | /ligand="GTP" /ligand_id="ChEBI:CHEBI:37565 | D-T-P-G-H | ||||||||
BINDING | 130 | 133 | /ligand="GTP" /ligand_id="ChEBI:CHEBI:37565 | [NT]-K-[LIV]-D |
end case
Additional information
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case <OC:Bacteria>
Size range | 571-1183 amino acids |
end case
case <OC:Archaea>
Size range | 589-620 amino acids |
end case
Related rules | None |
Fusion | None |
Comments | Intein in some members. |