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HAMAP rule MF_00107

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General rule information [?]

Accession MF_00107
Dates 1-JUN-2001 (Created)
12-MAR-2024 (Last updated, Version 41)
Name IspF
Scope(s) Bacteria
Template(s) P62617 (ISPF_ECOLI); P44815 (ISPF_HAEIN); Q8RQP5 (ISPF_THET8); P9WKG5 (ISPF_MYCTU); Q63T71 (ISPF_BURPS); Q3JRA0 (ISPF_BURP1); Q06756 (ISPF_BACSU); Q5NFU1 (ISPF_FRATT); Q8ZMF7 (ISPF_SALTY); Q8EBR3 (ISPF_SHEON); Q8ZBP7 (ISPF_YERPE); [ Recover all ]
Triggered by HAMAP; MF_00107 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier ISPF
Protein name RecName: Full=2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase;
                 Short=MECPP-synthase;
                 Short=MECDP-synthase;
                 Short=MECPS;
                 EC=4.6.1.12;
Gene name Name=ispF;

Comments [?]

FUNCTIONInvolved in the biosynthesis of isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP), two major building blocks of isoprenoid compounds. Catalyzes the conversion of 4-diphosphocytidyl-2- C-methyl-D-erythritol 2-phosphate (CDP-ME2P) to 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-CPP) with a corresponding release of cytidine 5-monophosphate (CMP).
CATALYTIC ACTIVITY Reaction=4-CDP-2-C-methyl-D-erythritol 2-phosphate = 2-C-methyl-D- erythritol 2,4-cyclic diphosphate + CMP; Xref=Rhea:RHEA:23864, ChEBI:CHEBI:57919, ChEBI:CHEBI:58483, ChEBI:CHEBI:60377; EC=4.6.1.12;
COFACTOR Name=a divalent metal cation; Xref=ChEBI:CHEBI:60240; Note=Binds 1 divalent metal cation per subunit.;
PATHWAYIsoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5- phosphate: step 4/6.
SUBUNITHomotrimer.
SIMILARITYBelongs to the IspF family.

Keywords [?]

Isoprene biosynthesis
Lyase
Metal-binding

Gene Ontology [?]

GO:0008685; Molecular function:2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase activity
GO:0019288; Biological process:isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway

Cross-references [?]

Pfam PF02542; YgbB; 1;
NCBIfam TIGR00151; IspF; 1;
PROSITE PS01350; ISPF; 1;

Features [?]

From: ISPF_ECOLI (P62617)
Key From To Description Tag Condition FTGroup
BINDING 8 10 /ligand="4-CDP-2-C-methyl-D-erythritol 2-phosphate"
/ligand_id="ChEBI:CHEBI:57919"		 D-x-H
BINDING 34 35 /ligand="4-CDP-2-C-methyl-D-erythritol 2-phosphate"
/ligand_id="ChEBI:CHEBI:57919"		 H-S
BINDING 56 58 /ligand="4-CDP-2-C-methyl-D-erythritol 2-phosphate"
/ligand_id="ChEBI:CHEBI:57919"		 [DNEK]-[IVL]-[GA]
BINDING 61 65 /ligand="4-CDP-2-C-methyl-D-erythritol 2-phosphate"
/ligand_id="ChEBI:CHEBI:57919"		 F-x-[DNEQ]-x-[DNEQ]
BINDING 100 106 /ligand="4-CDP-2-C-methyl-D-erythritol 2-phosphate"
/ligand_id="ChEBI:CHEBI:57919"		 [ALVI]-x(2)-P-[KNR]-M-[LAGI]
BINDING 132 135 /ligand="4-CDP-2-C-methyl-D-erythritol 2-phosphate"
/ligand_id="ChEBI:CHEBI:57919"		 [TS]-[TS]-x-[EDN]
BINDING 8 8 /ligand="a divalent metal cation"
/ligand_id="ChEBI:CHEBI:60240"		 D
BINDING 10 10 /ligand="a divalent metal cation"
/ligand_id="ChEBI:CHEBI:60240"		 H
BINDING 42 42 /ligand="a divalent metal cation"
/ligand_id="ChEBI:CHEBI:60240"		 H
BINDING 139 139 /ligand="4-CDP-2-C-methyl-D-erythritol 2-phosphate"
/ligand_id="ChEBI:CHEBI:57919"		 F
BINDING 142 142 /ligand="4-CDP-2-C-methyl-D-erythritol 2-phosphate"
/ligand_id="ChEBI:CHEBI:57919"		 [RHK]
SITE 34 34 /note="Transition state stabilizer" [HDN]
SITE 133 133 /note="Transition state stabilizer" [TS]

Additional information [?]

Size range 152-184 amino acids
Related rules MF_01520
Fusion Nter: None Cter: None
Comments See MF_01520 for bifunctional ispDF



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