HAMAP rule MF_00107

General rule information [?]

Accession	MF_00107
Dates	1-JUN-2001 (Created) 19-NOV-2022 (Last updated, Version 39)

Name

IspF

Scope

Bacteria

Templates

P62617 (ISPF_ECOLI); P44815 (ISPF_HAEIN); Q8RQP5 (ISPF_THET8); P9WKG5 (ISPF_MYCTU); Q63T71 (ISPF_BURPS); Q3JRA0 (ISPF_BURP1); Q06756 (ISPF_BACSU); Q5NFU1 (ISPF_FRATT); Q8ZMF7 (ISPF_SALTY); Q8EBR3 (ISPF_SHEON); Q8ZBP7 (ISPF_YERPE): [Recover all]

Triggered by

HAMAP; MF_00107 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier

ISPF

Protein name

RecName:		Full=2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase;
		Short=MECPP-synthase;
		Short=MECDP-synthase;
		Short=MECPS;
		EC 4.6.1.12;

Gene name

ispF

Comments [?]

Function	Involved in the biosynthesis of isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP), two major building blocks of isoprenoid compounds. Catalyzes the conversion of 4-diphosphocytidyl-2-C-methyl-D-erythritol 2-phosphate (CDP-ME2P) to 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-CPP) with a corresponding release of cytidine 5-monophosphate (CMP).
Catalytic activity	RHEA:23864: 4-CDP-2-C-methyl-D-erythritol 2-phosphate = 2-C-methyl-D-erythritol 2,4-cyclic diphosphate + CMP EC 4.6.1.12
Cofactor	a divalent metal cation Note: Binds 1 divalent metal cation per subunit.
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 4/6.
Subunit	Homotrimer.
Similarity	Belongs to the IspF family.

Keywords [?]

Isoprene biosynthesis
Lyase
Metal-binding

Gene Ontology [?]

GO:0008685; Molecular function: 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase activity.
GO:0016114; Biological process: terpenoid biosynthetic process.

Cross-references [?]

Pfam	PF02542; YgbB; 1;
TIGRFAMs	TIGR00151; IspF; 1;
PROSITE	PS01350; ISPF; 1;

Features [?]

From: ISPF_ECOLI (P62617)

Key From To Description Tag Condition FTGroup

BINDING 8 10 /ligand="4-CDP-2-C-methyl-D-erythritol 2-phosphate" /ligand_id="ChEBI:CHEBI:57919 D-x-H

BINDING 34 35 /ligand="4-CDP-2-C-methyl-D-erythritol 2-phosphate" /ligand_id="ChEBI:CHEBI:57919 H-S

BINDING 56 58 /ligand="4-CDP-2-C-methyl-D-erythritol 2-phosphate" /ligand_id="ChEBI:CHEBI:57919 [DNEK]-[IVL]-[GA]

BINDING 61 65 /ligand="4-CDP-2-C-methyl-D-erythritol 2-phosphate" /ligand_id="ChEBI:CHEBI:57919 F-x-[DNEQ]-x-[DNEQ]

BINDING 100 106 /ligand="4-CDP-2-C-methyl-D-erythritol 2-phosphate" /ligand_id="ChEBI:CHEBI:57919 [ALVI]-x(2)-P-[KNR]-M-[LAGI]

BINDING 132 135 /ligand="4-CDP-2-C-methyl-D-erythritol 2-phosphate" /ligand_id="ChEBI:CHEBI:57919 [TS]-[TS]-x-[EDN]

BINDING 8 8 /ligand="a divalent metal cation" /ligand_id="ChEBI:CHEBI:60240 D

BINDING 10 10 /ligand="a divalent metal cation" /ligand_id="ChEBI:CHEBI:60240 H

BINDING 42 42 /ligand="a divalent metal cation" /ligand_id="ChEBI:CHEBI:60240 H

BINDING 139 139 /ligand="4-CDP-2-C-methyl-D-erythritol 2-phosphate" /ligand_id="ChEBI:CHEBI:57919 F

BINDING 142 142 /ligand="4-CDP-2-C-methyl-D-erythritol 2-phosphate" /ligand_id="ChEBI:CHEBI:57919 [RHK]

SITE 34 34 Transition state stabilizer [HDN]

SITE 133 133 Transition state stabilizer [TS]

Additional information [?]

Size range	152-184 amino acids
Related rules	MF_01520 (ISPDF supersedes the current rule)
Fusion	None
Comments	See MF_01520 for bifunctional ispDF

View rule in raw text format (no links)