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HAMAP rule MF_00107

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General rule information [?]

Accession MF_00107
Dates 1-JUN-2001 (Created)
19-NOV-2022 (Last updated, Version 39)
Name IspF
Scope
Bacteria
Templates P62617 (ISPF_ECOLI); P44815 (ISPF_HAEIN); Q8RQP5 (ISPF_THET8); P9WKG5 (ISPF_MYCTU); Q63T71 (ISPF_BURPS); Q3JRA0 (ISPF_BURP1); Q06756 (ISPF_BACSU); Q5NFU1 (ISPF_FRATT); Q8ZMF7 (ISPF_SALTY); Q8EBR3 (ISPF_SHEON); Q8ZBP7 (ISPF_YERPE): [Recover all]

Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
ISPF
Protein name
RecName: Full=2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase;
Short=MECPP-synthase;
Short=MECDP-synthase;
Short=MECPS;
EC 4.6.1.12;
Gene name
ispF

Comments [?]

Function Involved in the biosynthesis of isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP), two major building blocks of isoprenoid compounds. Catalyzes the conversion of 4-diphosphocytidyl-2-C-methyl-D-erythritol 2-phosphate (CDP-ME2P) to 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-CPP) with a corresponding release of cytidine 5-monophosphate (CMP).
Catalytic activity RHEA:23864: 4-CDP-2-C-methyl-D-erythritol 2-phosphate = 2-C-methyl-D-erythritol 2,4-cyclic diphosphate + CMP
EC 4.6.1.12
Cofactor a divalent metal cation
Note: Binds 1 divalent metal cation per subunit.
Pathway Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 4/6.
Subunit Homotrimer.
Similarity Belongs to the IspF family.

Keywords [?]


Gene Ontology [?]

GO:0008685; Molecular function: 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase activity.
GO:0016114; Biological process: terpenoid biosynthetic process.

Cross-references [?]

Pfam PF02542; YgbB; 1;
TIGRFAMs TIGR00151; IspF; 1;
PROSITE PS01350; ISPF; 1;

Features [?]

From: ISPF_ECOLI (P62617)
Key     From     To       Description   Tag   Condition   FTGroup
BINDING     8     10       /ligand="4-CDP-2-C-methyl-D-erythritol 2-phosphate" /ligand_id="ChEBI:CHEBI:57919     D-x-H  
BINDING     34     35       /ligand="4-CDP-2-C-methyl-D-erythritol 2-phosphate" /ligand_id="ChEBI:CHEBI:57919     H-S  
BINDING     56     58       /ligand="4-CDP-2-C-methyl-D-erythritol 2-phosphate" /ligand_id="ChEBI:CHEBI:57919     [DNEK]-[IVL]-[GA]  
BINDING     61     65       /ligand="4-CDP-2-C-methyl-D-erythritol 2-phosphate" /ligand_id="ChEBI:CHEBI:57919     F-x-[DNEQ]-x-[DNEQ]  
BINDING     100     106       /ligand="4-CDP-2-C-methyl-D-erythritol 2-phosphate" /ligand_id="ChEBI:CHEBI:57919     [ALVI]-x(2)-P-[KNR]-M-[LAGI]  
BINDING     132     135       /ligand="4-CDP-2-C-methyl-D-erythritol 2-phosphate" /ligand_id="ChEBI:CHEBI:57919     [TS]-[TS]-x-[EDN]  
BINDING     8     8       /ligand="a divalent metal cation" /ligand_id="ChEBI:CHEBI:60240     D  
BINDING     10     10       /ligand="a divalent metal cation" /ligand_id="ChEBI:CHEBI:60240     H  
BINDING     42     42       /ligand="a divalent metal cation" /ligand_id="ChEBI:CHEBI:60240     H  
BINDING     139     139       /ligand="4-CDP-2-C-methyl-D-erythritol 2-phosphate" /ligand_id="ChEBI:CHEBI:57919     F  
BINDING     142     142       /ligand="4-CDP-2-C-methyl-D-erythritol 2-phosphate" /ligand_id="ChEBI:CHEBI:57919     [RHK]  
SITE     34     34       Transition state stabilizer     [HDN]  
SITE     133     133       Transition state stabilizer     [TS]  

Additional information [?]

Size range 152-184 amino acids
Related rules MF_01520 (ISPDF supersedes the current rule)
Fusion None
Comments See MF_01520 for bifunctional ispDF