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Annotation rule MF_00107
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General rule information [?]

Accession MF_00107
Dates 1-JUN-2001 (Created)
18-NOV-2019 (Last updated, Version 35)
Name IspF
Scope
Bacteria
Templates P62617 (ISPF_ECOLI); P44815 (ISPF_HAEIN); Q8RQP5 (ISPF_THET8); P9WKG5 (ISPF_MYCTU); Q63T71 (ISPF_BURPS); Q3JRA0 (ISPF_BURP1); Q06756 (ISPF_BACSU); Q5NFU1 (ISPF_FRATT); Q8ZMF7 (ISPF_SALTY); Q8EBR3 (ISPF_SHEON); Q8ZBP7 (ISPF_YERPE): [Recover all]
Triggered by

Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
ISPF
Protein name
RecName: Full=2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase;
Short=MECPP-synthase;
Short=MECDP-synthase;
Short=MECPS;
EC 4.6.1.12;
Gene name
ispF

Comments [?]

Function Involved in the biosynthesis of isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP), two major building blocks of isoprenoid compounds. Catalyzes the conversion of 4-diphosphocytidyl-2-C-methyl-D-erythritol 2-phosphate (CDP-ME2P) to 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-CPP) with a corresponding release of cytidine 5-monophosphate (CMP).
Catalytic activity RHEA:23864: 4-CDP-2-C-methyl-D-erythritol 2-phosphate = 2-C-methyl-D-erythritol 2,4-cyclic diphosphate + CMP
EC 4.6.1.12
Cofactor a divalent metal cation
Note: Binds 1 divalent metal cation per subunit.
Pathway Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 4/6.
Subunit Homotrimer.
Similarity Belongs to the IspF family.

Keywords [?]

Isoprene biosynthesis
Lyase
Metal-binding

Gene Ontology [?]

GO:0008685; Molecular function: 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase activity.
GO:0016114; Biological process: terpenoid biosynthetic process.

Cross-references [?]

Pfam PF02542; YgbB; 1;
TIGRFAMs TIGR00151; IspF; 1;
PROSITE PS01350; ISPF; 1;

Features [?]

From: ISPF_ECOLI (P62617)
Key     From     To       Description   Tag   Condition   FTGroup
REGION     8     10       Substrate binding     D-x-H  
REGION     34     35       Substrate binding     H-S  
REGION     38     46       Substrate binding     D-x(3)-H-x(3)-[DN]  
REGION     56     58       Substrate binding     [DNEK]-[IVL]-[GA]  
REGION     61     65       Substrate binding     F-x-[DNEQ]-x-[DNEQ]  
REGION     100     106       Substrate binding     [ALVI]-x(2)-P-[KNR]-M-[LAGI]  
REGION     131     135       Substrate binding     [AG]-[TS]-[TS]-x-[EDN]  
METAL     8     8       Divalent metal cation     D  
METAL     10     10       Divalent metal cation     H  
METAL     42     42       Divalent metal cation     H  
SITE     34     34       Transition state stabilizer     [HDN]  
SITE     133     133       Transition state stabilizer     [TS]  
BINDING     65     65       Substrate; via carbonyl oxygen     [DENQ]  
BINDING     139     139       Substrate; via carbonyl oxygen     F  
BINDING     142     142       Substrate     [RHK]  

Additional information [?]

Size range 152-184 amino acids
Related rules MF_01520 (ISPDF supersedes the current rule)
Fusion None
Comments See MF_01520 for bifunctional ispDF


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