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Annotation rule MF_00159
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General rule information [?]

Accession MF_00159
Dates 18-JAN-2002 (Created)
18-NOV-2019 (Last updated, Version 34)
Name IspG
Scope
Bacteria
Templates P62620 (ISPG_ECOLI); Q84GJ3 (ISPG_THETH); O67496 (ISPG_AQUAE); Q72H18 (ISPG_THET2): [Recover all]
Triggered by

Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
ISPG
case <OC:Cyanobacteria>
Protein name
RecName: Full=4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (ferredoxin);
EC 1.17.7.1;
AltName: Full=1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase;
else
Protein name
RecName: Full=4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (flavodoxin);
EC 1.17.7.3;
AltName: Full=1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase;
end case
Gene name
ispG

Comments [?]

Function Converts 2C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-2,4cPP) into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate.
case <OC:Cyanobacteria> or <OG:Chloroplast>
Catalytic activity RHEA:26119: (2E)-4-hydroxy-3-methylbut-2-enyl diphosphate + H2O + 2 oxidized [2Fe-2S]-[ferredoxin] = 2-C-methyl-D-erythritol 2,4-cyclic diphosphate + H(+) + 2 reduced [2Fe-2S]-[ferredoxin]
EC 1.17.7.1
else
Catalytic activity RHEA:43604: (2E)-4-hydroxy-3-methylbut-2-enyl diphosphate + 2 H(+) + H2O + oxidized [flavodoxin] = 2-C-methyl-D-erythritol 2,4-cyclic diphosphate + reduced [flavodoxin]
EC 1.17.7.3
end case
case <FTGroup:1>
Cofactor [4Fe-4S] cluster
Note: Binds 1 [4Fe-4S] cluster.
end case
Pathway Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 5/6.
Similarity Belongs to the IspG family.

Keywords [?]

case <FTGroup:1>
4Fe-4S
Metal-binding
Iron
Iron-sulfur
end case
Isoprene biosynthesis
Oxidoreductase

Gene Ontology [?]

case <FTGroup:1>
GO:0051539; Molecular function: 4 iron, 4 sulfur cluster binding.
end case
GO:0046429; Molecular function: 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase activity.
GO:0016114; Biological process: terpenoid biosynthetic process.

Cross-references [?]

Pfam PF04551; GcpE; 1;
TIGRFAMs TIGR00612; IspG_gcpE; 1;

Features [?]

From: ISPG_AQUAE (O67496)
Key     From     To       Description   Tag   Condition   FTGroup
METAL     265     265       Iron-sulfur (4Fe-4S)     C   1
METAL     268     268       Iron-sulfur (4Fe-4S)     C   1
METAL     300     300       Iron-sulfur (4Fe-4S)     C   1
METAL     307     307       Iron-sulfur (4Fe-4S)     E   1

Additional information [?]

Size range 344-663 amino acids
Related rules None
Fusion None
Comments Insertion in BACFN, BACFR, BACTN, CHLAB, CHLCV, CHLMU, CHLPN, CHLTE, CHLTR, LEPIC, LEPIN, PARUW and PORGI. Possible wrong start in CHLTE, DESVH and RHOPA.


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