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HAMAP rule MF_00159

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General rule information [?]

Accession MF_00159
Dates 18-JAN-2002 (Created)
1-JUN-2023 (Last updated, Version 38)
Name IspG
Scope
Bacteria
Templates P62620 (ISPG_ECOLI); Q84GJ3 (ISPG_THETH); O67496 (ISPG_AQUAE); Q72H18 (ISPG_THET2): [Recover all]
Triggered by

Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
ISPG
case <OC:Cyanobacteriota>
Protein name
RecName: Full=4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (ferredoxin);
EC 1.17.7.1;
AltName: Full=1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase;
else
Protein name
RecName: Full=4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (flavodoxin);
EC 1.17.7.3;
AltName: Full=1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase;
end case
Gene name
ispG

Comments [?]

Function Converts 2C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-2,4cPP) into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate.
case <OC:Cyanobacteriota> or <OG:Chloroplast>
Catalytic activity RHEA:26119: (2E)-4-hydroxy-3-methylbut-2-enyl diphosphate + H2O + 2 oxidized [2Fe-2S]-[ferredoxin] = 2-C-methyl-D-erythritol 2,4-cyclic diphosphate + H(+) + 2 reduced [2Fe-2S]-[ferredoxin]
EC 1.17.7.1
else
Catalytic activity RHEA:43604: (2E)-4-hydroxy-3-methylbut-2-enyl diphosphate + 2 H(+) + H2O + oxidized [flavodoxin] = 2-C-methyl-D-erythritol 2,4-cyclic diphosphate + reduced [flavodoxin]
EC 1.17.7.3
end case
case <FTGroup:1>
Cofactor [4Fe-4S] cluster
Note: Binds 1 [4Fe-4S] cluster.
end case
Pathway Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 5/6.
Similarity Belongs to the IspG family.

Keywords [?]

case <FTGroup:1>
4Fe-4S
Metal-binding
Iron
Iron-sulfur
end case
Isoprene biosynthesis
Oxidoreductase

Gene Ontology [?]

case <FTGroup:1>
GO:0051539; Molecular function: 4 iron, 4 sulfur cluster binding.
end case
GO:0046429; Molecular function: 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase activity.
GO:0016114; Biological process: terpenoid biosynthetic process.

Cross-references [?]

Pfam PF04551; GcpE; 1;
NCBIfam TIGR00612; IspG_gcpE; 1;

Features [?]

From: ISPG_AQUAE (O67496)
Key     From     To       Description   Tag   Condition   FTGroup
BINDING     265     265       /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883     C   1
BINDING     268     268       /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883     C   1
BINDING     300     300       /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883     C   1
BINDING     307     307       /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883     E   1

Additional information [?]

Size range 344-663 amino acids
Related rules None
Fusion None
Comments Insertion in BACFN, BACFR, BACTN, CHLAB, CHLCV, CHLMU, CHLPN, CHLTE, CHLTR, LEPIC, LEPIN, PARUW and PORGI. Possible wrong start in CHLTE, DESVH and RHOPA.


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