HAMAP rule MF_00177
General rule information
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| PURL | https://purl.expasy.org/hamap/rule/MF_00177 |
| Accession | MF_00177 |
| Dates | 28-FEB-2005 (Created)
02-SEP-2024 (Last updated, Version 27) |
| Name | Lys_tRNA_synth_class1 |
| Scope(s) |
Bacteria Archaea |
| Template(s) | O57963; O51603; P0CW67; [ Recover all ] |
| Triggered by |
HAMAP; MF_00177 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | SYK |
| Protein name | RecName: Full=Lysine--tRNA ligase; EC=6.1.1.6; AltName: Full=Lysyl-tRNA synthetase; Short=LysRS; |
| Gene name | Name=lysS; |
Comments
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| CATALYTIC ACTIVITY | Reaction=tRNA(Lys) + L-lysine + ATP = L-lysyl-tRNA(Lys) + AMP + diphosphate; Xref=Rhea:RHEA:20792, Rhea:RHEA-COMP:9696, Rhea:RHEA- COMP:9697, ChEBI:CHEBI:30616, ChEBI:CHEBI:32551, ChEBI:CHEBI:33019, ChEBI:CHEBI:78442, ChEBI:CHEBI:78529, ChEBI:CHEBI:456215; EC=6.1.1.6; |
| SUBCELLULAR LOCATION | Cytoplasm. |
| SIMILARITY | Belongs to the class-I aminoacyl-tRNA synthetase family. |
Keywords
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Gene Ontology
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| GO:0005524; Molecular function:ATP binding |
| GO:0004824; Molecular function:lysine-tRNA ligase activity |
| GO:0006430; Biological process:lysyl-tRNA aminoacylation |
| GO:0005737; Cellular component:cytoplasm |
Cross-references
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| Pfam | PF01921; tRNA-synt_1f; 1; |
| NCBIfam | TIGR00467; LysS_arch; 1; |
| PROSITE | PS00178; AA_TRNA_LIGASE_I; 1; |
Features
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| From: SYK_RICPR (Q9ZDF8) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| MOTIF | 44 | 52 | /note="'HIGH' region" | [AIPTV]-S-G-x-[ILPQV]-H-[ILMV]-G-[HNRST] | ||||||||
| MOTIF | 290 | 294 | /note="'KMSKS' region" | [ADKP]-[FILM]-[HKS]-[GKS]-S | ||||||||
| BINDING | 293 | 293 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616" |
K | ||||||||
Additional information
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| Size range | 493-580 amino acids |
| Related rules |
None |
| Fusion | Nter: None Cter: None |
| Comments | Lysyl-tRNA synthesis is catalyzed by two unrelated families of tRNA synthetase; class-I or class-II. Usually only one or the other is present, however Methanosarcina seem to have both. |