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HAMAP rule MF_00265

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General rule information [?]

Accession MF_00265
Dates 1-JUN-2001 (Created)
19-NOV-2022 (Last updated, Version 32)
Name VapC/Nob1
Scope
Bacteria
Archaea
Templates Q8ZM86 (VAPC_SALTY); O06662 (VAPC_SHIFL); P9WFB7 (VAPC3_MYCTU); P9WF85 (VPC25_MYCTU); P9WFB9 (VAPC2_MYCTU); O58440 (NOB1_PYRHO): [Recover all]
Triggered by

Propagated annotation [?]


Identifier, protein and gene names [?]

case <OC:Escherichia> or <OC:Shigella> or <OC:Salmonella>
Identifier
VAPC
Protein name
RecName: Full=tRNA(fMet)-specific endonuclease VapC;
EC 3.1.-.-;
AltName: Full=RNase VapC;
AltName: Full=Toxin VapC;
Gene name
vapC
else case <OC:Bacteria>
Identifier
VAPC
Protein name
RecName: Full=Ribonuclease VapC;
Short=RNase VapC;
EC 3.1.-.-;
AltName: Full=Toxin VapC;
Gene name
vapC
else case <OC:Archaea>
Identifier
VAPC
Protein name
RecName: Full=Ribonuclease VapC;
Short=RNase VapC;
EC 3.1.-.-;
AltName: Full=Putative toxin VapC;
Gene name
vapC
end case

Comments [?]

case <OC:Escherichia> or <OC:Shigella> or <OC:Salmonella>
Function Toxic component of a toxin-antitoxin (TA) system. A site-specific tRNA-(fMet) endonuclease, it cleaves both charged and uncharged tRNA-(fMet) between positions 38 and 39 at the anticodon stem-loop boundary. Its toxic effects are neutralized by expression of cognate antitoxin VapB.
else
Function Toxic component of a toxin-antitoxin (TA) system. An RNase.
end case
case <FT:1> or <FT:2>
Cofactor Mg(2+)
end case
Similarity Belongs to the PINc/VapC protein family.

Keywords [?]

Toxin-antitoxin system
case <FT:1> or <FT:2>
Magnesium
Metal-binding
end case
Hydrolase
Nuclease
Toxin

Gene Ontology [?]

case <FT:1> or <FT:2>
GO:0000287; Molecular function: magnesium ion binding.
end case

Cross-references [?]

Pfam PF01850; PIN; 0-1; trigger=Yes;
case not <Feature:PF01850>
SMART SM00670; PINc; 0-1; trigger=YesYes;
end case

Features [?]

From: VAPC1_AQUAE (O66399)
Key     From     To       Description   Tag   Condition   FTGroup
BINDING (Optional)     9     9       /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420     D  
BINDING (Optional)     102     102       /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420     D  

Additional information [?]

Size range 112-197 amino acids
Related rules None
Fusion Nter: <Leu-rich domain>; Cter: <TRAM domain>
Comments VAPC1_THEAC and NOB1_METJA may be too long at N-terminus. Y540_THEAC fused with a Leu-rich transmembrane domain at N-terminus and TRAM domain at C-terminus, several other proteins are also fused to a TRAM domain. VAPC8_MYCTU atypical sequence. VAPC3_METJA, VPC14_MYCTU too short. At least 1 zinc-binding archaeal member processes pre-16S rRNA (NOB1_PYRHO, O58440); archaeal members that have the ability to bind zinc in the C-termini have been accordingly reannotated.


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