HAMAP rule MF_00265
General rule information
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| Accession | MF_00265 |
| Dates | 1-JUN-2001 (Created)
19-NOV-2022 (Last updated, Version 32) |
| Name | VapC/Nob1 |
| Scope(s) |
Bacteria Archaea |
| Template(s) | Q8ZM86 (VAPC_SALTY); O06662 (VAPC_SHIFL); P9WFB7 (VAPC3_MYCTU); P9WF85 (VPC25_MYCTU); P9WFB9 (VAPC2_MYCTU); O58440 (NOB1_PYRHO); [ Recover all ] |
| Triggered by |
HAMAP; MF_00265 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| case <OC:Escherichia> or <OC:Shigella> or <OC:Salmonella> | |
| Identifier | VAPC |
| Protein name | RecName: Full=tRNA(fMet)-specific endonuclease VapC; EC=3.1.-.-; AltName: Full=RNase VapC; AltName: Full=Toxin VapC; |
| Gene name | Name=vapC; |
| else case <OC:Bacteria> | |
| Identifier | VAPC |
| Protein name | RecName: Full=Ribonuclease VapC; Short=RNase VapC; EC=3.1.-.-; AltName: Full=Toxin VapC; |
| Gene name | Name=vapC; |
| else case <OC:Archaea> | |
| Identifier | VAPC |
| Protein name | RecName: Full=Ribonuclease VapC; Short=RNase VapC; EC=3.1.-.-; AltName: Full=Putative toxin VapC; |
| Gene name | Name=vapC; |
| end case | |
Comments
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| case <OC:Escherichia> or <OC:Shigella> or <OC:Salmonella> | |
| FUNCTION | Toxic component of a toxin-antitoxin (TA) system. A site- specific tRNA-(fMet) endonuclease, it cleaves both charged and uncharged tRNA-(fMet) between positions 38 and 39 at the anticodon stem-loop boundary. Its toxic effects are neutralized by expression of cognate antitoxin VapB. |
| else | |
| FUNCTION | Toxic component of a toxin-antitoxin (TA) system. An RNase. |
| end case | |
| case <FT:1> or <FT:2> | |
| COFACTOR | Name=Mg(2+); Xref=ChEBI:CHEBI:18420; |
| end case | |
| SIMILARITY | Belongs to the PINc/VapC protein family. |
Keywords
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| Toxin-antitoxin system | |
| case <FT:1> or <FT:2> | |
| Magnesium | |
| Metal-binding | |
| end case | |
| Hydrolase | |
| Nuclease | |
| Toxin | |
Gene Ontology
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| case <FT:1> or <FT:2> | |
| GO:0000287; Molecular function:magnesium ion binding | |
| end case | |
Cross-references
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Features
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| From: VAPC1_AQUAE (O66399) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| BINDING | 9 | 9 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" |
D | ||||||||
| BINDING | 102 | 102 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" |
D | ||||||||
Additional information
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| Size range | 112-197 amino acids |
| Related rules |
None |
| Fusion | Nter: <Leu-rich domain> Cter: <TRAM domain> |
| Comments | VAPC1_THEAC and NOB1_METJA may be too long at N-terminus. Y540_THEAC fused with a Leu-rich transmembrane domain at N-terminus and TRAM domain at C-terminus, several other proteins are also fused to a TRAM domain. VAPC8_MYCTU atypical sequence. VAPC3_METJA, VPC14_MYCTU too short. At least 1 zinc-binding archaeal member processes pre-16S rRNA (NOB1_PYRHO, O58440); archaeal members that have the ability to bind zinc in the C-termini have been accordingly reannotated. |