HAMAP rule MF_00394
General rule information
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| Accession | MF_00394 |
| Dates | 1-JUN-2001 (Created)
15-FEB-2024 (Last updated, Version 40) |
| Name | NAD_Glyc3P_dehydrog |
| Scope(s) |
Bacteria Archaea |
| Template(s) | P0A6S7 (GPDA_ECOLI); A0A0F6AK91 (GPDA_THESQ); P46919 (GPDA_BACSU); O29390 (GPDA_ARCFU); [ Recover all ] |
| Triggered by |
HAMAP; MF_00394 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | GPDA |
| Protein name | RecName: Full=Glycerol-3-phosphate dehydrogenase [NAD(P)+]; EC=1.1.1.94; AltName: Full=NAD(P)(+)-dependent glycerol-3-phosphate dehydrogenase; AltName: Full=NAD(P)H-dependent dihydroxyacetone-phosphate reductase; |
| Gene name | Name=gpsA; |
Comments
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| case <OC:Bacteria> | |
| FUNCTION | Catalyzes the reduction of the glycolytic intermediate dihydroxyacetone phosphate (DHAP) to sn-glycerol 3-phosphate (G3P), the key precursor for phospholipid synthesis. |
| else case <OC:Archaea> | |
| FUNCTION | Catalyzes the reduction of the glycolytic intermediate dihydroxyacetone phosphate (DHAP) to sn-glycerol 3-phosphate (G3P). |
| end case | |
| CATALYTIC ACTIVITY | Reaction=NAD(+) + sn-glycerol 3-phosphate = dihydroxyacetone phosphate + H(+) + NADH; Xref=Rhea:RHEA:11092, ChEBI:CHEBI:15378, ChEBI:CHEBI:57540, ChEBI:CHEBI:57597, ChEBI:CHEBI:57642, ChEBI:CHEBI:57945; EC=1.1.1.94; PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:11094; |
| CATALYTIC ACTIVITY | Reaction=NADP(+) + sn-glycerol 3-phosphate = dihydroxyacetone phosphate + H(+) + NADPH; Xref=Rhea:RHEA:11096, ChEBI:CHEBI:15378, ChEBI:CHEBI:57597, ChEBI:CHEBI:57642, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349; EC=1.1.1.94; PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:11098; |
| SUBCELLULAR LOCATION | Cytoplasm. |
| case <OC:Bacteria> | |
| PATHWAY | Membrane lipid metabolism; glycerophospholipid metabolism. |
| end case | |
| SIMILARITY | Belongs to the NAD-dependent glycerol-3-phosphate dehydrogenase family. |
Keywords
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| Cytoplasm | |
| case <OC:Bacteria> | |
| Lipid biosynthesis | |
| Lipid metabolism | |
| Phospholipid biosynthesis | |
| Phospholipid metabolism | |
| end case | |
| NAD | |
| NADP | |
| Oxidoreductase | |
| Nucleotide-binding | |
Gene Ontology
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| GO:0047952; Molecular function:glycerol-3-phosphate dehydrogenase [NAD(P)+] activity |
| GO:0046167; Biological process:glycerol-3-phosphate biosynthetic process |
| GO:0006650; Biological process:glycerophospholipid metabolic process |
| GO:0005737; Cellular component:cytoplasm |
Cross-references
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| Pfam | PF07479; NAD_Gly3P_dh_C; 1; |
| Pfam | PF01210; NAD_Gly3P_dh_N; 1; |
| PIRSF | PIRSF000114; Glycerol-3-P_dh; 1; |
| PRINTS | PR00077; GPDHDRGNASE; 1; |
| PROSITE | PS00957; NAD_G3PDH; 1; |
Features
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| From: GPDA_THESQ (A0A0F6AK91) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| ACT_SITE | 177 | 177 | /note="Proton acceptor" | K | ||||||||
| BINDING | 10 | 10 | /ligand="NADPH" /ligand_id="ChEBI:CHEBI:57783" |
[ST] | ||||||||
| BINDING | 11 | 11 | /ligand="NADPH" /ligand_id="ChEBI:CHEBI:57783" |
[WYF] | ||||||||
| BINDING | 31 | 31 | /ligand="NADPH" /ligand_id="ChEBI:CHEBI:57783" |
[RHK] | ||||||||
| BINDING | 32 | 32 | /ligand="NADPH" /ligand_id="ChEBI:CHEBI:57783" |
[RHK] | ||||||||
| BINDING | 47 | 47 | /ligand="NADPH" /ligand_id="ChEBI:CHEBI:57783" |
Y | ||||||||
| BINDING | 98 | 98 | /ligand="NADPH" /ligand_id="ChEBI:CHEBI:57783" |
K | ||||||||
| BINDING | 98 | 98 | /ligand="sn-glycerol 3-phosphate" /ligand_id="ChEBI:CHEBI:57597" |
K | ||||||||
| BINDING | 125 | 125 | /ligand="sn-glycerol 3-phosphate" /ligand_id="ChEBI:CHEBI:57597" |
G | ||||||||
| BINDING | 127 | 127 | /ligand="sn-glycerol 3-phosphate" /ligand_id="ChEBI:CHEBI:57597" |
[ST] | ||||||||
| BINDING | 129 | 129 | /ligand="NADPH" /ligand_id="ChEBI:CHEBI:57783" |
[AGS] | ||||||||
| BINDING | 177 | 177 | /ligand="sn-glycerol 3-phosphate" /ligand_id="ChEBI:CHEBI:57597" |
K | ||||||||
| BINDING | 230 | 230 | /ligand="sn-glycerol 3-phosphate" /ligand_id="ChEBI:CHEBI:57597" |
D | ||||||||
| BINDING | 240 | 240 | /ligand="sn-glycerol 3-phosphate" /ligand_id="ChEBI:CHEBI:57597" |
S | ||||||||
| BINDING | 241 | 241 | /ligand="NADPH" /ligand_id="ChEBI:CHEBI:57783" |
R | ||||||||
| BINDING | 241 | 241 | /ligand="sn-glycerol 3-phosphate" /ligand_id="ChEBI:CHEBI:57597" |
R | ||||||||
| BINDING | 242 | 242 | /ligand="sn-glycerol 3-phosphate" /ligand_id="ChEBI:CHEBI:57597" |
N | ||||||||
| BINDING | 265 | 265 | /ligand="NADPH" /ligand_id="ChEBI:CHEBI:57783" |
[VLI] | ||||||||
| BINDING | 267 | 267 | /ligand="NADPH" /ligand_id="ChEBI:CHEBI:57783" |
E | ||||||||
Additional information
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| Size range | 297-369 amino acids |
| Related rules |
None |
| Fusion | Nter: None Cter: None |