HAMAP rule MF_00449
General rule information
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| Accession | MF_00449 |
| Dates | 17-SEP-2001 (Created)
19-NOV-2022 (Last updated, Version 31) |
| Name | RAD50 |
| Scope(s) |
Archaea |
| Template(s) | P58301 (RAD50_PYRFU); [ Recover all ] |
| Triggered by |
HAMAP; MF_00449 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | RAD50 |
| Protein name | RecName: Full=DNA double-strand break repair Rad50 ATPase; |
| Gene name | Name=rad50; |
Comments
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| case <OC:Halobacteria> | |
| FUNCTION | Part of the Rad50/Mre11 complex, which is involved in the early steps of DNA double-strand break (DSB) repair. Rad50 controls the balance between DNA end bridging and DNA resection via ATP-dependent structural rearrangements of the Rad50/Mre11 complex. |
| else | |
| FUNCTION | Part of the Rad50/Mre11 complex, which is involved in the early steps of DNA double-strand break (DSB) repair. The complex may facilitate opening of the processed DNA ends to aid in the recruitment of HerA and NurA. Rad50 controls the balance between DNA end bridging and DNA resection via ATP-dependent structural rearrangements of the Rad50/Mre11 complex. |
| end case | |
| case <FTGroup:1> | |
| COFACTOR | Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Note=Binds 1 zinc ion per homodimer.; |
| DOMAIN | The two conserved Cys that bind zinc constitute the zinc-hook, which separates the large intramolecular coiled coil regions. The 2 Cys residues coordinate one molecule of zinc with the help of the 2 Cys residues of the zinc-hook of another Rad50 molecule, thereby forming a V-shaped homodimer. |
| end case | |
| SUBUNIT | Homodimer. Forms a heterotetramer composed of two Mre11 subunits and two Rad50 subunits. |
| SIMILARITY | Belongs to the SMC family. RAD50 subfamily. |
Keywords
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| ATP-binding | |
| DNA damage | |
| DNA repair | |
| Hydrolase | |
| Nucleotide-binding | |
| case <FTGroup:1> | |
| Metal-binding | |
| Zinc | |
| end case | |
Gene Ontology
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| GO:0005524; Molecular function:ATP binding | |
| GO:0016887; Molecular function:ATP hydrolysis activity | |
| GO:0006302; Biological process:double-strand break repair | |
| case <FTGroup:1> | |
| GO:0008270; Molecular function:zinc ion binding | |
| end case | |
Cross-references
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| PROSITE | PS51131; ZN_HOOK; 1; |
| Pfam | PF04423; Rad50_zn_hook; 1; |
| Pfam | PF02463; SMC_N; 1; |
| General | Coiled_coil; -; 0-unlimited; |
Features
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| From: RAD50_PYRFU (P58301) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| BINDING | 32 | 38 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616" |
N-[GA]-[SA]-G-K-[ST]-[ST] | ||||||||
| BINDING | 791 | 796 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616" |
[FLIM]-[LIVFM]-S-G-G-E | ||||||||
| BINDING | 444 | 444 | /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" |
C | 1 | |||||||
| BINDING | 447 | 447 | /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" |
C | 1 | |||||||
| BINDING | 12 | 12 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616" |
[RK] | ||||||||
| BINDING | 140 | 140 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616" |
Q | ||||||||
Additional information
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| Size range | 830-1100 amino acids |
| Related rules |
None |
| Fusion | Nter: None Cter: None |