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HAMAP rule MF_00449

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General rule information [?]

Accession MF_00449
Dates 17-SEP-2001 (Created)
19-NOV-2022 (Last updated, Version 31)
Name RAD50
Scope
Archaea
Template P58301 (RAD50_PYRFU)
Triggered by

Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
RAD50
Protein name
RecName: Full=DNA double-strand break repair Rad50 ATPase;
Gene name
rad50

Comments [?]

case <OC:Halobacteria>
Function Part of the Rad50/Mre11 complex, which is involved in the early steps of DNA double-strand break (DSB) repair. Rad50 controls the balance between DNA end bridging and DNA resection via ATP-dependent structural rearrangements of the Rad50/Mre11 complex.
else
Function Part of the Rad50/Mre11 complex, which is involved in the early steps of DNA double-strand break (DSB) repair. The complex may facilitate opening of the processed DNA ends to aid in the recruitment of HerA and NurA. Rad50 controls the balance between DNA end bridging and DNA resection via ATP-dependent structural rearrangements of the Rad50/Mre11 complex.
end case
case <FTGroup:1>
Cofactor Zn(2+)
Note: Binds 1 zinc ion per homodimer.
Domain The two conserved Cys that bind zinc constitute the zinc-hook, which separates the large intramolecular coiled coil regions. The 2 Cys residues coordinate one molecule of zinc with the help of the 2 Cys residues of the zinc-hook of another Rad50 molecule, thereby forming a V-shaped homodimer.
end case
Subunit Homodimer. Forms a heterotetramer composed of two Mre11 subunits and two Rad50 subunits.
Similarity Belongs to the SMC family. RAD50 subfamily.

Keywords [?]

ATP-binding
DNA damage
DNA repair
Hydrolase
Nucleotide-binding
case <FTGroup:1>
Metal-binding
Zinc
end case

Gene Ontology [?]

GO:0005524; Molecular function: ATP binding.
GO:0016887; Molecular function: ATP hydrolysis activity.
GO:0006302; Biological process: double-strand break repair.
case <FTGroup:1>
GO:0008270; Molecular function: zinc ion binding.
end case

Cross-references [?]

PROSITE PS51131; ZN_HOOK; 1; trigger=PRU00471;
Pfam PF04423; Rad50_zn_hook; 1;
PF02463; SMC_N; 1;

Computed features [?]

General Coiled_coil; -; 0-unlimited; trigger=yes;

Features [?]

From: RAD50_PYRFU (P58301)
Key     From     To       Description   Tag   Condition   FTGroup
BINDING (Optional)     32     38       /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616     N-[GA]-[SA]-G-K-[ST]-[ST]  
BINDING (Optional)     791     796       /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616     [FLIM]-[LIVFM]-S-G-G-E  
BINDING     444     444       /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105     C   1
BINDING     447     447       /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105     C   1
BINDING (Optional)     12     12       /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616     [RK]  
BINDING (Optional)     140     140       /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616     Q  

Additional information [?]

Size range 830-1100 amino acids
Related rules None
Fusion None


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