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Annotation rule MF_00537
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General rule information [?]

Accession MF_00537
Dates 24-NOV-2006 (Created)
18-NOV-2019 (Last updated, Version 14)
Name Ribosomal_S14_1
Scope
Bacteria
Plastid
Templates P0AG59 (RS14_ECOLI); P0DOY6 (RS14Z_THET8): [Recover all]
case <OC:Bacteria> or <OG:Chloroplast>
end case


Propagated annotation [?]


Identifier, protein and gene names [?]

case <OC:Bacteria> and not <OC:Cyanobacteria>
Identifier
RS14
Protein name
RecName: Full=30S ribosomal protein S14;
Gene name
rpsN
else case <OC:Cyanobacteria>
Identifier
RS14
Protein name
RecName: Full=30S ribosomal protein S14;
Gene name
rpsN, rps14
else case <OG:Chloroplast>
Identifier
RR14
Protein name
RecName: Full=30S ribosomal protein S14, chloroplastic;
Gene name
rps14
end case

Comments [?]

case <OC:Bacteria>
Function Binds 16S rRNA, required for the assembly of 30S particles and may also be responsible for determining the conformation of the 16S rRNA at the A site.
Subunit Part of the 30S ribosomal subunit. Contacts proteins S3 and S10.
end case
case <OG:Chloroplast>
Function Binds 16S rRNA, required for the assembly of 30S particles.
Subunit Part of the 30S ribosomal subunit.
Subcellular location Plastid, chloroplast.
end case
Similarity Belongs to the universal ribosomal protein uS14 family.

Keywords [?]


Gene Ontology [?]

GO:0006412; Biological process: translation.
GO:0019843; Molecular function: rRNA binding.
case <OG:Chloroplast>
GO:0009507; Cellular component: chloroplast.
end case

Cross-references [?]

PROSITE PS00527; RIBOSOMAL_S14; 1;
Pfam PF00253; Ribosomal_S14; 1;

Additional information [?]

Size range 89-103 amino acids
Related rules MF_01364 (RS14Z)
Fusion None
Comments In bacteria this gene has been subject to horizontal gene transfer, subsequent differential gene loss and insertions/deletions near the N-terminus. The ancestral version is most likely the shortest one (group I of PubMed 11102698) which has conserved cysteine residues that in the case of Thermus thermophilus have been shown to bind zinc. We have chosen to use the identifier RS14Z in bacteria for the zinc-binding subfamily (MF_01364). The group II proteins have an approximately 23 amino acid insertion and the group III proteins have an approximately 38 residue insertion; we do not distinguish between them however (this family, MF_00537); members of this subfamily do not usually possess the conserved cysteine residues. Many bacteria possess more than one copy of RS14, usually one of which is able to bind zinc. Interestingly, in the proteobacteria (which usually have the group III form) the acquired form is found within the spc operon, signifying gene replacement took place within the operon. The proteins in Legionella species and SALRD match this family but have the conserved residues necessary to bind zinc; whether they do so is unknown. See: PubMed=11102698; Brochier C., Philippe H., Moreira D.; "The evolutionary history of ribosomal protein RpS14: horizontal gene transfer at the heart of the ribosome."; Trends Genet. 16:529-533(2000). PubMed=11574053; Makarova K.S., Ponomarev V.A., Koonin E.V.; "Two C or not two C: recurrent disruption of Zn-ribbons, gene duplication, lineage-specific gene loss, and horizontal gene transfer in evolution of bacterial ribosomal proteins."; Genome Biol. 2:RESEARCH 0033-RESEARCH 0033(2001).