HAMAP rule MF_00785
General rule information
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| PURL | https://purl.expasy.org/hamap/rule/MF_00785 |
| Accession | MF_00785 |
| Dates | 28-FEB-2005 (Created)
02-SEP-2024 (Last updated, Version 27) |
| Name | CbiX |
| Scope(s) |
Archaea |
| Template(s) | O29537; O27448; P61816; Q8TJZ5; [ Recover all ] |
| Triggered by |
HAMAP; MF_00785 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| case <OC:Methanobacteria> or <OC:Methanococci> or <OC:Methanomicrobia> or <OC:Methanopyri> | |
| Identifier | CFBA |
| Protein name | RecName: Full=Sirohydrochlorin cobaltochelatase; EC=4.99.1.3; AltName: Full=CbiXS; AltName: Full=Sirohydrochlorin nickelchelatase; EC=4.99.1.11; |
| Gene name | Name=cbiX; Synonyms=cfbA; |
| else | |
| Identifier | CBIX |
| Protein name | RecName: Full=Sirohydrochlorin cobaltochelatase; EC=4.99.1.3; AltName: Full=CbiXS; |
| Gene name | Name=cbiX; |
| end case | |
Comments
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| case <OC:Methanobacteria> or <OC:Methanococci> or <OC:Methanomicrobia> or <OC:Methanopyri> | |
| FUNCTION | Catalyzes the insertion of Co(2+) into sirohydrochlorin as part of the anaerobic pathway to cobalamin biosynthesis. Involved in the biosynthesis of the unique nickel-containing tetrapyrrole coenzyme F430, the prosthetic group of methyl-coenzyme M reductase (MCR), which plays a key role in methanogenesis and anaerobic methane oxidation. Catalyzes the insertion of Ni(2+) into sirohydrochlorin to yield Ni- sirohydrochlorin. |
| CATALYTIC ACTIVITY | Reaction=Co-sirohydrochlorin + 2 H(+) = sirohydrochlorin + Co(2+); Xref=Rhea:RHEA:15893, ChEBI:CHEBI:15378, ChEBI:CHEBI:48828, ChEBI:CHEBI:58351, ChEBI:CHEBI:60049; EC=4.99.1.3; |
| CATALYTIC ACTIVITY | Reaction=Ni-sirohydrochlorin + 2 H(+) = sirohydrochlorin + Ni(2+); Xref=Rhea:RHEA:52796, ChEBI:CHEBI:15378, ChEBI:CHEBI:49786, ChEBI:CHEBI:58351, ChEBI:CHEBI:136841; EC=4.99.1.11; |
| else | |
| FUNCTION | Catalyzes the insertion of Co(2+) into sirohydrochlorin as part of the anaerobic pathway to cobalamin biosynthesis. |
| CATALYTIC ACTIVITY | Reaction=Co-sirohydrochlorin + 2 H(+) = sirohydrochlorin + Co(2+); Xref=Rhea:RHEA:15893, ChEBI:CHEBI:15378, ChEBI:CHEBI:48828, ChEBI:CHEBI:58351, ChEBI:CHEBI:60049; EC=4.99.1.3; |
| end case | |
| PATHWAY | Cofactor biosynthesis; adenosylcobalamin biosynthesis; cob(II)yrinate a,c-diamide from sirohydrochlorin (anaerobic route): step 1/10. |
| SUBUNIT | Homotetramer; dimer of dimers. |
| SIMILARITY | Belongs to the CbiX family. CbiXS subfamily. |
Keywords
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| Cobalamin biosynthesis | |
| Lyase | |
| case <OC:Methanobacteria> or <OC:Methanococci> or <OC:Methanomicrobia> or <OC:Methanopyri> | |
| Methanogenesis | |
| end case | |
| case <FT:2> and <OC:Methanobacteria> or <OC:Methanococci> or <OC:Methanomicrobia> or <OC:Methanopyri> | |
| Metal-binding | |
| Cobalt | |
| Nickel | |
| else | |
| Metal-binding | |
| Cobalt | |
| end case | |
Gene Ontology
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| GO:0016852; Molecular function:sirohydrochlorin cobaltochelatase activity | |
| GO:0019251; Biological process:anaerobic cobalamin biosynthetic process | |
| case <FT:2> and <OCellular component:Methanobacteria> or <OC:Methanococci> or <OC:Methanomicrobia> or <OC:Methanopyri> | |
| GO:0050897; Molecular function:cobalt ion binding | |
| GO:0016151; Molecular function:nickel cation binding | |
| else; https://www.ebi.ac.uk/QuickGO/term/else | |
| GO:0050897; Molecular function:cobalt ion binding | |
| end case | |
Cross-references
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| Pfam | PF01903; CbiX; 1; |
Features
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| From: CBIX_ARCFU (O29537) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| BINDING | 69 | 74 | /ligand="substrate" | |||||||||
| ACT_SITE | 10 | 10 | /note="Proton acceptor" | H | ||||||||
| case <OC:Methanobacteria> or <OC:Methanococci> or <OC:Methanomicrobia> or <OC:Methanopyri> | ||||||||||||
| BINDING | 10 | 10 | /ligand="Co(2+)" /ligand_id="ChEBI:CHEBI:48828" |
H | ||||||||
| BINDING | 10 | 10 | /ligand="Ni(2+)" /ligand_id="ChEBI:CHEBI:49786" |
H | ||||||||
| BINDING | 74 | 74 | /ligand="Co(2+)" /ligand_id="ChEBI:CHEBI:48828" |
H | ||||||||
| BINDING | 74 | 74 | /ligand="Ni(2+)" /ligand_id="ChEBI:CHEBI:49786" |
H | ||||||||
| else | ||||||||||||
| BINDING | 10 | 10 | /ligand="Co(2+)" /ligand_id="ChEBI:CHEBI:48828" |
H | ||||||||
| BINDING | 74 | 74 | /ligand="Co(2+)" /ligand_id="ChEBI:CHEBI:48828" |
H | ||||||||
| end case | ||||||||||||
| BINDING | 46 | 46 | /ligand="substrate" | [RKQE] | ||||||||
Additional information
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| Size range | 120-144 amino acids |
| Related rules |
None |
| Fusion | Nter: None Cter: None |
| Comments | HALSA has a protein with two tandem copies of a cbiX domain, resembling CbiK arrangement. |