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HAMAP rule MF_00785

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General rule information [?]

Accession MF_00785
Dates 24-MAY-2004 (Created)
19-NOV-2022 (Last updated, Version 29)
Name CbiX
Scope
Archaea
Templates O29537 (CBIX_ARCFU); O27448 (CFBA_METTH); P61816 (CFBA_METBF); Q8TJZ5 (CFBA_METAC): [Recover all]
Triggered by

Propagated annotation [?]


Identifier, protein and gene names [?]

case <OC:Methanobacteria> or <OC:Methanococci> or <OC:Methanomicrobia> or <OC:Methanopyri>
Identifier
CFBA
Protein name
RecName: Full=Sirohydrochlorin cobaltochelatase;
EC 4.99.1.3;
AltName: Full=CbiXS;
AltName: Full=Sirohydrochlorin nickelchelatase;
EC 4.99.1.11;
Gene name
cbiX, cfbA
else
Identifier
CBIX
Protein name
RecName: Full=Sirohydrochlorin cobaltochelatase;
EC 4.99.1.3;
AltName: Full=CbiXS;
Gene name
cbiX
end case

Comments [?]

case <OC:Methanobacteria> or <OC:Methanococci> or <OC:Methanomicrobia> or <OC:Methanopyri>
Function Catalyzes the insertion of Co(2+) into sirohydrochlorin as part of the anaerobic pathway to cobalamin biosynthesis. Involved in the biosynthesis of the unique nickel-containing tetrapyrrole coenzyme F430, the prosthetic group of methyl-coenzyme M reductase (MCR), which plays a key role in methanogenesis and anaerobic methane oxidation. Catalyzes the insertion of Ni(2+) into sirohydrochlorin to yield Ni-sirohydrochlorin.
Catalytic activity RHEA:15893: Co-sirohydrochlorin + 2 H(+) = Co(2+) + sirohydrochlorin
EC 4.99.1.3
RHEA:52796: 2 H(+) + Ni-sirohydrochlorin = Ni(2+) + sirohydrochlorin
EC 4.99.1.11
else
Function Catalyzes the insertion of Co(2+) into sirohydrochlorin as part of the anaerobic pathway to cobalamin biosynthesis.
Catalytic activity RHEA:15893: Co-sirohydrochlorin + 2 H(+) = Co(2+) + sirohydrochlorin
EC 4.99.1.3
end case
Pathway Cofactor biosynthesis; adenosylcobalamin biosynthesis; cob(II)yrinate a,c-diamide from sirohydrochlorin (anaerobic route): step 1/10.
Subunit Homotetramer; dimer of dimers.
Similarity Belongs to the CbiX family. CbiXS subfamily.

Keywords [?]

Cobalamin biosynthesis
Lyase
case <OC:Methanobacteria> or <OC:Methanococci> or <OC:Methanomicrobia> or <OC:Methanopyri>
Methanogenesis
end case
case <FT:2> and <OC:Methanobacteria> or <OC:Methanococci> or <OC:Methanomicrobia> or <OC:Methanopyri>
Metal-binding
Cobalt
Nickel
else
Metal-binding
Cobalt
end case

Gene Ontology [?]

GO:0016852; Molecular function: sirohydrochlorin cobaltochelatase activity.
GO:0019251; Biological process: anaerobic cobalamin biosynthetic process.
case <FT:2> and <OC:Methanobacteria> or <OC:Methanococci> or <OC:Methanomicrobia> or <OC:Methanopyri>
GO:0050897; Molecular function: cobalt ion binding.
GO:0016151; Molecular function: nickel cation binding.
else
GO:0050897; Molecular function: cobalt ion binding.
end case

Cross-references [?]

Pfam PF01903; CbiX; 1;

Features [?]

From: CBIX_ARCFU (O29537)
Key     From     To       Description   Tag   Condition   FTGroup
BINDING     69     74       /ligand="substrate        
ACT_SITE     10     10       Proton acceptor     H  
case <OC:Methanobacteria> or <OC:Methanococci> or <OC:Methanomicrobia> or <OC:Methanopyri>
BINDING     10     10       /ligand="Co(2+)" /ligand_id="ChEBI:CHEBI:48828     H  
BINDING     10     10       /ligand="Ni(2+)" /ligand_id="ChEBI:CHEBI:49786     H  
BINDING     74     74       /ligand="Co(2+)" /ligand_id="ChEBI:CHEBI:48828     H  
BINDING     74     74       /ligand="Ni(2+)" /ligand_id="ChEBI:CHEBI:49786     H  
else
BINDING     10     10       /ligand="Co(2+)" /ligand_id="ChEBI:CHEBI:48828     H  
BINDING     74     74       /ligand="Co(2+)" /ligand_id="ChEBI:CHEBI:48828     H  
end case
BINDING     46     46       /ligand="substrate     [RKQE]  

Additional information [?]

Size range 120-144 amino acids
Related rules None
Fusion None
Comments HALSA has a protein with two tandem copies of a cbiX domain, resembling CbiK arrangement.


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