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HAMAP rule MF_00978

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General rule information [?]

Accession MF_00978
Dates 9-DEC-2013 (Created)
1-JUN-2023 (Last updated, Version 13)
Name Bifunct_BirA
Scope(s) Bacteria
Template(s) P06709 (BIRA_ECOLI); [ Recover all ]
Triggered by
case c? <OC:Bacteria>
HAMAP; MF_00978 (Get profile general information and statistics)
end case

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier BIRA
case <OC:Gammaproteobacteria>
Protein name RecName: Full=Bifunctional ligase/repressor BirA;
AltName: Full=Biotin--[acetyl-CoA-carboxylase] ligase;
                 EC=6.3.4.15;
AltName: Full=Biotin--protein ligase;
AltName: Full=Biotin-[acetyl-CoA carboxylase] synthetase;
AltName: Full=Biotin operon repressor;
else
Protein name RecName: Full=Bifunctional ligase/repressor BirA;
AltName: Full=Biotin--[acetyl-CoA-carboxylase] ligase;
                 EC=6.3.4.15;
AltName: Full=Biotin--protein ligase;
AltName: Full=Biotin-[acetyl-CoA carboxylase] synthetase;
end case
Gene name Name=birA;

Comments [?]

case <OC:Gammaproteobacteria>
FUNCTIONActs both as a biotin--[acetyl-CoA-carboxylase] ligase and a biotin-operon repressor. In the presence of ATP, BirA activates biotin to form the BirA-biotinyl-5'-adenylate (BirA-bio-5'-AMP or holoBirA) complex. HoloBirA can either transfer the biotinyl moiety to the biotin carboxyl carrier protein (BCCP) subunit of acetyl-CoA carboxylase, or bind to the biotin operator site and inhibit transcription of the operon.
else
FUNCTIONActs both as a biotin--[acetyl-CoA-carboxylase] ligase and a repressor.
end case
CATALYTIC ACTIVITY Reaction=ATP + biotin + L-lysyl-[protein] = AMP + diphosphate + H(+) + N(6)-biotinyl-L-lysyl-[protein]; Xref=Rhea:RHEA:11756, Rhea:RHEA- COMP:9752, Rhea:RHEA-COMP:10505, ChEBI:CHEBI:15378, ChEBI:CHEBI:29969, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57586, ChEBI:CHEBI:83144, ChEBI:CHEBI:456215; EC=6.3.4.15;
SIMILARITYBelongs to the biotin--protein ligase family.

Keywords [?]


Gene Ontology [?]

GO:0005524; Molecular function:ATP binding
GO:0004077; Molecular function:biotin-[acetyl-CoA-carboxylase] ligase activity
GO:0003677; Molecular function:DNA binding
GO:0006355; Biological process:regulation of DNA-templated transcription

Cross-references [?]

PROSITE PS51733; BPL_LPL_CATALYTIC; 1;
Pfam PF02237; BPL_C; 1;
Pfam PF03099; BPL_LplA_LipB; 1;
Pfam PF08279; HTH_11; 1;
NCBIfam TIGR00121; birA_ligase; 1;
NCBIfam TIGR00122; birA_repr_reg; 1;

Features [?]

From: BIRA_ECOLI (P06709)
Key From To Description Tag Condition FTGroup
DNA_BIND 22 41 /note="H-T-H motif"
BINDING 89 91 /ligand="biotin"
/ligand_id="ChEBI:CHEBI:57586"
[ST]-[ST]-N
BINDING 116 118 /ligand="biotin"
/ligand_id="ChEBI:CHEBI:57586"
R-G-R
BINDING 112 112 /ligand="biotin"
/ligand_id="ChEBI:CHEBI:57586"
Q
BINDING 183 183 /ligand="biotin"
/ligand_id="ChEBI:CHEBI:57586"
K

Additional information [?]

Size range 280-400 amino acids
Related rules None
Fusion Nter: None Cter: None



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