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HAMAP rule MF_00978

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General rule information [?]

Accession MF_00978
Dates 9-DEC-2013 (Created)
1-JUN-2023 (Last updated, Version 13)
Name Bifunct_BirA
Scope
Bacteria
Template P06709 (BIRA_ECOLI)
case <OC:Bacteria>
Triggered by
end case


Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
BIRA
case <OC:Gammaproteobacteria>
Protein name
RecName: Full=Bifunctional ligase/repressor BirA;
AltName: Full=Biotin--[acetyl-CoA-carboxylase] ligase;
EC 6.3.4.15;
AltName: Full=Biotin--protein ligase;
AltName: Full=Biotin-[acetyl-CoA carboxylase] synthetase;
AltName: Full=Biotin operon repressor;
else
Protein name
RecName: Full=Bifunctional ligase/repressor BirA;
AltName: Full=Biotin--[acetyl-CoA-carboxylase] ligase;
EC 6.3.4.15;
AltName: Full=Biotin--protein ligase;
AltName: Full=Biotin-[acetyl-CoA carboxylase] synthetase;
end case
Gene name
birA

Comments [?]

case <OC:Gammaproteobacteria>
Function Acts both as a biotin--[acetyl-CoA-carboxylase] ligase and a biotin-operon repressor. In the presence of ATP, BirA activates biotin to form the BirA-biotinyl-5'-adenylate (BirA-bio-5'-AMP or holoBirA) complex. HoloBirA can either transfer the biotinyl moiety to the biotin carboxyl carrier protein (BCCP) subunit of acetyl-CoA carboxylase, or bind to the biotin operator site and inhibit transcription of the operon.
else
Function Acts both as a biotin--[acetyl-CoA-carboxylase] ligase and a repressor.
end case
Catalytic activity RHEA:11756: ATP + biotin + L-lysyl-[protein] = AMP + diphosphate + H(+) + N(6)-biotinyl-L-lysyl-[protein]
EC 6.3.4.15
Similarity Belongs to the biotin--protein ligase family.

Keywords [?]

ATP-binding
Biotin
DNA-binding
Ligase
Nucleotide-binding
Repressor
Transcription
Transcription regulation

Gene Ontology [?]

GO:0005524; Molecular function: ATP binding.
GO:0004077; Molecular function: biotin-[acetyl-CoA-carboxylase] ligase activity.
GO:0003677; Molecular function: DNA binding.
GO:0006355; Biological process: regulation of DNA-templated transcription.

Cross-references [?]

PROSITE PS51733; BPL_LPL_CATALYTIC; 1; trigger=PRU01067;
Pfam PF02237; BPL_C; 1;
PF03099; BPL_LplA_LipB; 1;
PF08279; HTH_11; 1;
NCBIfam TIGR00121; birA_ligase; 1;
TIGR00122; birA_repr_reg; 1;

Features [?]

From: BIRA_ECOLI (P06709)
Key     From     To       Description   Tag   Condition   FTGroup
DNA_BIND     22     41       H-T-H motif        
BINDING (Optional)     89     91       /ligand="biotin" /ligand_id="ChEBI:CHEBI:57586     [ST]-[ST]-N  
BINDING (Optional)     116     118       /ligand="biotin" /ligand_id="ChEBI:CHEBI:57586     R-G-R  
BINDING (Optional)     112     112       /ligand="biotin" /ligand_id="ChEBI:CHEBI:57586     Q  
BINDING (Optional)     183     183       /ligand="biotin" /ligand_id="ChEBI:CHEBI:57586     K  

Additional information [?]

Size range 280-400 amino acids
Related rules None
Fusion None


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