HAMAP rule MF_01120
General rule information
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Accession | MF_01120 |
Dates | 24-DEC-2003 (Created)
1-JUN-2023 (Last updated, Version 38) |
Name | LysK |
Scope(s) |
Archaea Bacteria Chloroflexota Deinococcota |
Template(s) | Q8VUS5 (LYSK_THET2); Q4JAP7 (LYSK_SULAC); [ Recover all ] |
Triggered by |
HAMAP; MF_01120 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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Identifier | LYSK |
case <OC:Sulfolobales> | |
Protein name | RecName: Full=[LysW]-lysine/[LysW]-ornithine hydrolase; EC=3.5.1.130; |
else case <OC:Archaea> and not <OC:Sulfolobales> | |
Protein name | RecName: Full=Putative [LysW]-lysine/[LysW]-ornithine hydrolase; EC=3.5.1.130; EC=3.5.1.132; |
else case <OC:Deinococcota> | |
Protein name | RecName: Full=[LysW]-lysine hydrolase; EC=3.5.1.130; |
else case <OC:Chloroflexota> | |
Protein name | RecName: Full=Putative [LysW]-lysine hydrolase; EC=3.5.1.130; |
end case | |
Gene name | Name=lysK; |
Comments
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case <OC:Archaea> | |
FUNCTION | Catalyzes the release of L-lysine from [LysW]-gamma-L-lysine and the release of L-ornithine from [LysW]-L-ornithine. |
else case <OC:Bacteria> | |
FUNCTION | Catalyzes the release of L-lysine from [LysW]-gamma-L-lysine. |
end case | |
CATALYTIC ACTIVITY | Reaction=[amino-group carrier protein]-C-terminal-gamma-(L-lysyl)-L- glutamate + H2O = [amino-group carrier protein]-C-terminal-L- glutamate + L-lysine; Xref=Rhea:RHEA:48684, Rhea:RHEA-COMP:9693, Rhea:RHEA-COMP:9715, ChEBI:CHEBI:15377, ChEBI:CHEBI:32551, ChEBI:CHEBI:78525, ChEBI:CHEBI:78526; EC=3.5.1.130; |
case <OC:Archaea> | |
CATALYTIC ACTIVITY | Reaction=[amino-group carrier protein]-C-terminal-gamma-(L-ornithyl)-L- glutamate + H2O = [amino-group carrier protein]-C-terminal-L- glutamate + L-ornithine; Xref=Rhea:RHEA:52676, Rhea:RHEA-COMP:9693, Rhea:RHEA-COMP:13328, ChEBI:CHEBI:15377, ChEBI:CHEBI:46911, ChEBI:CHEBI:78525, ChEBI:CHEBI:136763; EC=3.5.1.132; |
end case | |
COFACTOR | Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Name=Co(2+); Xref=ChEBI:CHEBI:48828; Note=Binds 2 Zn(2+) or Co(2+) ions per subunit.; |
PATHWAY | Amino-acid biosynthesis; L-lysine biosynthesis via AAA pathway; L-lysine from L-alpha-aminoadipate (Thermus route): step 5/5. |
case <OC:Archaea> | |
PATHWAY | Amino-acid biosynthesis; L-arginine biosynthesis. |
end case | |
SUBCELLULAR LOCATION | Cytoplasm. |
SIMILARITY | Belongs to the peptidase M20A family. LysK subfamily. |
Keywords
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Amino-acid biosynthesis | |
Cytoplasm | |
Hydrolase | |
Lysine biosynthesis | |
case <OC:Archaea> | |
Arginine biosynthesis | |
end case | |
Metal-binding | |
Cobalt | |
Zinc |
Gene Ontology
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GO:0050897; Molecular function:cobalt ion binding | |
GO:0008270; Molecular function:zinc ion binding | |
GO:0016811; Molecular function:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides | |
case <OCellular component:Archaea> | |
GO:0042450; Biological process:arginine biosynthetic process via ornithine | |
end case | |
GO:0019878; Biological process:lysine biosynthetic process via aminoadipic acid | |
GO:0005737; Cellular component:cytoplasm |
Cross-references
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Pfam | PF01546; Peptidase_M20; 1; |
NCBIfam | TIGR01902; DapE-lys-deAc; 1; |
PROSITE | PS00758; ARGE_DAPE_CPG2_1; 1; |
PROSITE | PS00759; ARGE_DAPE_CPG2_2; 1; |
Features
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From: LYSK_THET2 (Q8VUS5) | ||||||||||||
Key | From | To | Description | Tag | Condition | FTGroup | ||||||
ACT_SITE | 69 | 69 | D | |||||||||
ACT_SITE | 124 | 124 | /note="Proton acceptor" | E | ||||||||
BINDING | 67 | 67 | /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_label="1" |
H | ||||||||
BINDING | 91 | 91 | /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_label="1" |
D | ||||||||
BINDING | 91 | 91 | /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_label="2" |
D | ||||||||
BINDING | 125 | 125 | /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_label="2" |
E | ||||||||
BINDING | 148 | 148 | /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_label="1" |
E | ||||||||
BINDING | 326 | 326 | /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_label="2" |
H |
Additional information
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Size range | 320-425 amino acids |
Related rules |
None |
Fusion | Nter: None Cter: None |