HAMAP rule MF_01120

General rule information [?]

PURL	https://purl.expasy.org/hamap/rule/MF_01120
Accession	MF_01120
Dates	28-FEB-2005 (Created) 13-JUN-2025 (Last updated, Version 37)
Name	LysK
Scope(s)	Archaea Bacteria Chloroflexota Deinococcota
Template(s)	Q8VUS5; Q4JAP7; [ Recover all ]
Triggered by	HAMAP; MF_01120 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier	LYSK
case <OC:Sulfolobales>
Protein name	RecName: Full=[LysW]-lysine/[LysW]-ornithine hydrolase; EC=3.5.1.130;
else case <OC:Archaea> and not <OC:Sulfolobales>
Protein name	RecName: Full=Putative [LysW]-lysine/[LysW]-ornithine hydrolase; EC=3.5.1.130; EC=3.5.1.132;
else case <OC:Deinococcota>
Protein name	RecName: Full=[LysW]-lysine hydrolase; EC=3.5.1.130;
else case <OC:Chloroflexota>
Protein name	RecName: Full=Putative [LysW]-lysine hydrolase; EC=3.5.1.130;
end case
Gene name	Name=lysK;

Comments [?]

case <OC:Archaea>
FUNCTION	Catalyzes the release of L-lysine from [LysW]-gamma-L-lysine and the release of L-ornithine from [LysW]-L-ornithine.
else case <OC:Bacteria>
FUNCTION	Catalyzes the release of L-lysine from [LysW]-gamma-L-lysine.
end case
CATALYTIC ACTIVITY	Reaction=[amino-group carrier protein]-C-terminal-gamma-(L-lysyl)-L- glutamate + H2O = [amino-group carrier protein]-C-terminal-L- glutamate + L-lysine; Xref=Rhea:RHEA:48684, Rhea:RHEA-COMP:9693, Rhea:RHEA-COMP:9715, ChEBI:CHEBI:15377, ChEBI:CHEBI:32551, ChEBI:CHEBI:78525, ChEBI:CHEBI:78526; EC=3.5.1.130;
case <OC:Archaea>
CATALYTIC ACTIVITY	Reaction=[amino-group carrier protein]-C-terminal-gamma-(L-ornithyl)-L- glutamate + H2O = [amino-group carrier protein]-C-terminal-L- glutamate + L-ornithine; Xref=Rhea:RHEA:52676, Rhea:RHEA-COMP:9693, Rhea:RHEA-COMP:13328, ChEBI:CHEBI:15377, ChEBI:CHEBI:46911, ChEBI:CHEBI:78525, ChEBI:CHEBI:136763; EC=3.5.1.132;
end case
COFACTOR	Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Name=Co(2+); Xref=ChEBI:CHEBI:48828; Note=Binds 2 Zn(2+) or Co(2+) ions per subunit.;
PATHWAY	Amino-acid biosynthesis; L-lysine biosynthesis via AAA pathway; L-lysine from L-alpha-aminoadipate (Thermus route): step 5/5.
case <OC:Archaea>
PATHWAY	Amino-acid biosynthesis; L-arginine biosynthesis.
end case
SUBCELLULAR LOCATION	Cytoplasm.
SIMILARITY	Belongs to the peptidase M20A family. LysK subfamily.

Keywords [?]

Gene Ontology [?]

GO:0050897; Molecular function:cobalt ion binding
GO:0008270; Molecular function:zinc ion binding
GO:0016811; Molecular function:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides
case <OCellular component:Archaea>
GO:0042450; Biological process:L-arginine biosynthetic process via ornithine
end case
GO:0019878; Biological process:lysine biosynthetic process via aminoadipic acid
GO:0005737; Cellular component:cytoplasm

Cross-references [?]

Features [?]

From: LYSK_THET2 (Q8VUS5)

Key

From

Description

Tag

Condition

FTGroup

ACT_SITE

124

/note="Proton acceptor"

BINDING

/ligand="Zn(2+)"
/ligand_id="ChEBI:CHEBI:29105"
/ligand_label="1"

BINDING

/ligand="Zn(2+)"
/ligand_id="ChEBI:CHEBI:29105"
/ligand_label="1"

BINDING

/ligand="Zn(2+)"
/ligand_id="ChEBI:CHEBI:29105"
/ligand_label="2"

BINDING

125

/ligand="Zn(2+)"
/ligand_id="ChEBI:CHEBI:29105"
/ligand_label="2"

BINDING

148

/ligand="Zn(2+)"
/ligand_id="ChEBI:CHEBI:29105"
/ligand_label="1"

BINDING

326

/ligand="Zn(2+)"
/ligand_id="ChEBI:CHEBI:29105"
/ligand_label="2"

Additional information [?]