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HAMAP rule MF_01129

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General rule information [?]

Accession MF_01129
Dates 22-JUL-2003 (Created)
1-JUN-2023 (Last updated, Version 46)
Name PPase_energized_pump
Scope
Bacteria
Archaea
Templates Q9S5X0 (HPPA_THEMA); Q3AFC6 (HPPA_CARHZ); Q8ZWI8 (HPPA_PYRAE); Q8PYZ8 (HPPA1_METMA): [Recover all]
Triggered by

Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
HPPA
case <FTTag:Kdep>
Protein name
RecName: Full=Putative K(+)-stimulated pyrophosphate-energized sodium pump;
EC 7.2.3.1;
AltName: Full=Membrane-bound sodium-translocating pyrophosphatase;
AltName: Full=Pyrophosphate-energized inorganic pyrophosphatase;
Short=Na(+)-PPase;
else case <FTTag:Kindep>
Protein name
RecName: Full=K(+)-insensitive pyrophosphate-energized proton pump;
EC 7.1.3.1;
AltName: Full=Membrane-bound proton-translocating pyrophosphatase;
AltName: Full=Pyrophosphate-energized inorganic pyrophosphatase;
Short=H(+)-PPase;
else
Protein name
RecName: Full=Pyrophosphate-energized proton pump;
EC 7.1.3.1;
AltName: Full=Membrane-bound proton-translocating pyrophosphatase;
AltName: Full=Pyrophosphate-energized inorganic pyrophosphatase;
Short=H(+)-PPase;
end case
Gene name
hppA

Comments [?]

case <FTTag:Kdep>
Function Sodium pump that utilizes the energy of pyrophosphate hydrolysis as the driving force for Na(+) movement across the membrane.
Catalytic activity RHEA:57884: diphosphate + H2O + Na(+)(in) = H(+) + Na(+)(out) + 2 phosphate
EC 7.2.3.1
else
Function Proton pump that utilizes the energy of pyrophosphate hydrolysis as the driving force for proton movement across the membrane. Generates a proton motive force.
Catalytic activity RHEA:13973: diphosphate + H(+)(in) + H2O = 2 H(+)(out) + 2 phosphate
EC 7.1.3.1
end case
Cofactor Mg(2+)
case <FTTag:Kdep>
Activity regulation Requires K(+) for maximal activity.
end case
Subunit Homodimer.
case not defined <Property:Membrane> or <Property:Membrane=1>
Subcellular location Cell membrane; Multi-pass membrane protein.
else case <Property:Membrane=2>
Subcellular location Cell inner membrane; Multi-pass membrane protein.
end case
case <FTTag:Kdep>
Similarity Belongs to the H(+)-translocating pyrophosphatase (TC 3.A.10) family. K(+)-stimulated subfamily.
else case <FTTag:Kindep>
Similarity Belongs to the H(+)-translocating pyrophosphatase (TC 3.A.10) family. K(+)-insensitive subfamily.
else
Similarity Belongs to the H(+)-translocating pyrophosphatase (TC 3.A.10) family.
end case

Keywords [?]

case defined <Property:Membrane> and <Property:Membrane=2>
Cell inner membrane
end case
Cell membrane
Ion transport
Magnesium
Membrane
case <FTTag:Kdep>
Potassium
Sodium
Sodium transport
else
Hydrogen ion transport
end case
Translocase
Transport
Transmembrane
Transmembrane helix

Gene Ontology [?]

GO:0009678; Molecular function: pyrophosphate hydrolysis-driven proton transmembrane transporter activity.
GO:0004427; Molecular function: inorganic diphosphate phosphatase activity.
GO:0000287; Molecular function: magnesium ion binding.
case <FTTag:Kdep>
GO:0030955; Molecular function: potassium ion binding.
GO:0006814; Biological process: sodium ion transport.
else
GO:1902600; Biological process: proton transmembrane transport.
end case
GO:0005886; Cellular component: plasma membrane.

Cross-references [?]

Pfam PF03030; H_PPase; 1;
NCBIfam TIGR01104; V_PPase; 1;
PIRSF PIRSF001265; H+-PPase; 1;

Computed features [?]

General Transmembrane; -; 13-18; trigger=yes;

Features [?]

From: HPPA_THEMA (Q9S5X0)
Key     From     To       Description   Tag   Condition   FTGroup
SITE (Optional)     495     495       Determinant of potassium dependence   Kdep   A  
From: HPPA_PYRAE (Q8ZWI8)
SITE (Optional)     476     476       Determinant of potassium independence   Kindep   K  

Additional information [?]

Size range 655-825 amino acids
Related rules None
Fusion None
Comments There are two classes of pyrophosphate-energized pumps, differing in K(+) sensitivity (PubMed:17454298). Three members of the K(+)-stimulated subfamily have been characterized as sodium pumps (PubMed:17605473). Further characterization is needed to distinguish Na(+) transporters from H(+) transporters.


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