HAMAP rule MF_01129
General rule information
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| PURL | https://purl.expasy.org/hamap/rule/MF_01129 |
| Accession | MF_01129 |
| Dates | 28-FEB-2005 (Created)
03-SEP-2024 (Last updated, Version 42) |
| Name | PPase_energized_pump |
| Scope(s) |
Bacteria Archaea |
| Template(s) | Q9S5X0; Q3AFC6; Q8ZWI8; Q8PYZ8; [ Recover all ] |
| Triggered by |
HAMAP; MF_01129 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | HPPA |
| case <FTTag:Kdep> | |
| Protein name | RecName: Full=Putative K(+)-stimulated pyrophosphate-energized sodium pump; EC=7.2.3.1; AltName: Full=Membrane-bound sodium-translocating pyrophosphatase; AltName: Full=Pyrophosphate-energized inorganic pyrophosphatase; Short=Na(+)-PPase; |
| else case <FTTag:Kindep> | |
| Protein name | RecName: Full=K(+)-insensitive pyrophosphate-energized proton pump; EC=7.1.3.1; AltName: Full=Membrane-bound proton-translocating pyrophosphatase; AltName: Full=Pyrophosphate-energized inorganic pyrophosphatase; Short=H(+)-PPase; |
| else | |
| Protein name | RecName: Full=Pyrophosphate-energized proton pump; EC=7.1.3.1; AltName: Full=Membrane-bound proton-translocating pyrophosphatase; AltName: Full=Pyrophosphate-energized inorganic pyrophosphatase; Short=H(+)-PPase; |
| end case | |
| Gene name | Name=hppA; |
Comments
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| case <FTTag:Kdep> | |
| FUNCTION | Sodium pump that utilizes the energy of pyrophosphate hydrolysis as the driving force for Na(+) movement across the membrane. |
| CATALYTIC ACTIVITY | Reaction=Na(+)(in) + diphosphate + H2O = Na(+)(out) + 2 phosphate + H(+); Xref=Rhea:RHEA:57884, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:29101, ChEBI:CHEBI:33019, ChEBI:CHEBI:43474; EC=7.2.3.1; |
| else | |
| FUNCTION | Proton pump that utilizes the energy of pyrophosphate hydrolysis as the driving force for proton movement across the membrane. Generates a proton motive force. |
| CATALYTIC ACTIVITY | Reaction=diphosphate + H2O + H(+)(in) = 2 phosphate + 2 H(+)(out); Xref=Rhea:RHEA:13973, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:33019, ChEBI:CHEBI:43474; EC=7.1.3.1; |
| end case | |
| COFACTOR | Name=Mg(2+); Xref=ChEBI:CHEBI:18420; |
| case <FTTag:Kdep> | |
| ACTIVITY REGULATION | Requires K(+) for maximal activity. |
| end case | |
| SUBUNIT | Homodimer. |
| case not defined <Property:Membrane> or <Property:Membrane=1> | |
| SUBCELLULAR LOCATION | Cell membrane; Multi-pass membrane protein. |
| else case <Property:Membrane=2> | |
| SUBCELLULAR LOCATION | Cell inner membrane; Multi-pass membrane protein. |
| end case | |
| case <FTTag:Kdep> | |
| SIMILARITY | Belongs to the H(+)-translocating pyrophosphatase (TC 3.A.10) family. K(+)-stimulated subfamily. |
| else case <FTTag:Kindep> | |
| SIMILARITY | Belongs to the H(+)-translocating pyrophosphatase (TC 3.A.10) family. K(+)-insensitive subfamily. |
| else | |
| SIMILARITY | Belongs to the H(+)-translocating pyrophosphatase (TC 3.A.10) family. |
| end case | |
Keywords
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| case defined <Property:Membrane> and <Property:Membrane=2> | |
| Cell inner membrane | |
| end case | |
| Cell membrane | |
| Ion transport | |
| Magnesium | |
| Membrane | |
| case <FTTag:Kdep> | |
| Potassium | |
| Sodium | |
| Sodium transport | |
| else | |
| Hydrogen ion transport | |
| end case | |
| Translocase | |
| Transport | |
| Transmembrane | |
| Transmembrane helix | |
Gene Ontology
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| GO:0009678; Molecular function:diphosphate hydrolysis-driven proton transmembrane transporter activity | |
| GO:0004427; Molecular function:inorganic diphosphate phosphatase activity | |
| GO:0000287; Molecular function:magnesium ion binding | |
| case <FTTag:Kdep> | |
| GO:0030955; Molecular function:potassium ion binding | |
| GO:0006814; Biological process:sodium ion transport | |
| else; https://www.ebi.ac.uk/QuickGO/term/else | |
| GO:1902600; Biological process:proton transmembrane transport | |
| end case | |
| GO:0005886; Cellular component:plasma membrane | |
Cross-references
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| Pfam | PF03030; H_PPase; 1; |
| NCBIfam | TIGR01104; V_PPase; 1; |
| PIRSF | PIRSF001265; H+-PPase; 1; |
| General | Transmembrane; -; 13-18; |
Features
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| From: HPPA_THEMA (Q9S5X0) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| SITE | 495 | 495 | /note="Determinant of potassium dependence" | Kdep | A | |||||||
| From: HPPA_PYRAE (Q8ZWI8) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| SITE | 476 | 476 | /note="Determinant of potassium independence" | Kindep | K | |||||||
Additional information
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| Size range | 655-825 amino acids |
| Related rules |
None |
| Fusion | Nter: None Cter: None |
| Comments | There are two classes of pyrophosphate-energized pumps, differing in K(+) sensitivity (PubMed:17454298). Three members of the K(+)-stimulated subfamily have been characterized as sodium pumps (PubMed:17605473). Further characterization is needed to distinguish Na(+) transporters from H(+) transporters. |