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HAMAP rule MF_01137

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General rule information [?]

Accession MF_01137
Dates 20-NOV-2003 (Created)
1-JUN-2023 (Last updated, Version 25)
Name CdhA
Scope(s) Archaea
Template(s) Q08368 (ACDA1_METTE); Q46G04 (ACDA1_METBF); [ Recover all ]
Triggered by HAMAP; MF_01137 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier ACDA
Protein name RecName: Full=Acetyl-CoA decarbonylase/synthase complex subunit alpha;
                 Short=ACDS complex subunit alpha;
                 EC=1.2.7.4;
AltName: Full=ACDS complex carbon monoxide dehydrogenase subunit alpha;
                 Short=ACDS CODH subunit alpha;
Gene name Name=cdhA;

Comments [?]

case <OC:Methanosarcinaceae>
FUNCTIONPart of the ACDS complex that catalyzes the reversible cleavage of acetyl-CoA, allowing growth on acetate as sole source of carbon and energy. The alpha-epsilon subcomponent functions as a carbon monoxide dehydrogenase.
end case
case not <OC:Methanosarcinaceae>
FUNCTIONPart of the ACDS complex that catalyzes the reversible cleavage of acetyl-CoA, allowing autotrophic growth from CO(2). The alpha-epsilon subcomponent functions as a carbon monoxide dehydrogenase.
end case
CATALYTIC ACTIVITY Reaction=CO + H2O + 2 oxidized [2Fe-2S]-[ferredoxin] = CO2 + 2 H(+) + 2 reduced [2Fe-2S]-[ferredoxin]; Xref=Rhea:RHEA:21040, Rhea:RHEA- COMP:10000, Rhea:RHEA-COMP:10001, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:17245, ChEBI:CHEBI:33737, ChEBI:CHEBI:33738; EC=1.2.7.4;
COFACTOR Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883; Note=Binds 7 [4Fe-4S] clusters per heterotetramer.;
COFACTOR Name=[Ni-4Fe-4S] cluster; Xref=ChEBI:CHEBI:47739; Note=Binds 2 [Ni-4Fe-4S] clusters per heterotetramer.;
case <OC:Methanosarcinaceae>
PATHWAYOne-carbon metabolism; methanogenesis from acetate.
end case
SUBUNITHeterotetramer of two alpha and two epsilon subunits. The ACDS complex is made up of alpha, epsilon, beta, gamma and delta subunits with a probable stoichiometry of (alpha(2)epsilon(2))(4)-beta(8)- (gamma(1)delta(1))(8).
DOMAINCluster B is an all-cysteinyl-liganded 4Fe-4S cluster; cluster C is a mixed Ni-Fe-S cluster which is the active site of CO oxidation. Cluster D is also an all-cysteinyl-liganded 4Fe-4S cluster that bridges the two subunits of the CODH dimer. Contains two additional 4Fe-4S clusters, dubbed E and F, that probably transport electrons from ferredoxin to the B cluster.
SIMILARITYBelongs to the Ni-containing carbon monoxide dehydrogenase family.

Keywords [?]

case <OC:Methanosarcinaceae>
Methanogenesis
end case
Oxidoreductase
Metal-binding
case <FTGroup:3>
Nickel
end case
case <FTGroup:1> or <FTGroup:2> or <FTGroup:3> or <FTGroup:4> or <FTGroup:5>
Iron
Iron-sulfur
end case
case <FTGroup:1> or <FTGroup:2> or <FTGroup:4> or <FTGroup:5>
4Fe-4S
end case

Gene Ontology [?]

case <FTGroup:1> or <FTGroup:2> or <FTGroup:3> or <FTGroup:4> or <FTGroup:5>
GO:0005506; Molecular function:iron ion binding
end case
case <FTGroup:3>
GO:0016151; Molecular function:nickel cation binding
end case
GO:0018492; Molecular function:carbon-monoxide dehydrogenase (acceptor) activity
case <OCellular component:Methanosarcinaceae>
GO:0019385; Biological process:methanogenesis, from acetate
end case

Cross-references [?]

Pfam PF00037; Fer4; 2;
Pfam PF03063; Prismane; 1;
PRINTS PR00353; 4FE4SFRDOXIN; 1;
NCBIfam TIGR00314; CdhA; 1;
PROSITE PS00198; 4FE4S_FER_1; 2;
PROSITE PS51379; 4FE4S_FER_2; 2;

Features [?]

From: ACDA1_METBF (Q46G04)
Key From To Description Tag Condition FTGroup
BINDING 73 73 /ligand="[4Fe-4S] cluster"
/ligand_id="ChEBI:CHEBI:49883"
/ligand_label="1"
/ligand_note="ligand shared between dimeric partners"		 C 1
BINDING 76 76 /ligand="[4Fe-4S] cluster"
/ligand_id="ChEBI:CHEBI:49883"
/ligand_label="2"		 C 2
BINDING 77 77 /ligand="[4Fe-4S] cluster"
/ligand_id="ChEBI:CHEBI:49883"
/ligand_label="1"
/ligand_note="ligand shared between dimeric partners"		 C 1
BINDING 79 79 /ligand="[4Fe-4S] cluster"
/ligand_id="ChEBI:CHEBI:49883"
/ligand_label="2"		 C 2
BINDING 84 84 /ligand="[4Fe-4S] cluster"
/ligand_id="ChEBI:CHEBI:49883"
/ligand_label="2"		 C 2
BINDING 94 94 /ligand="[4Fe-4S] cluster"
/ligand_id="ChEBI:CHEBI:49883"
/ligand_label="2"		 C 2
BINDING 250 250 /ligand="[Ni-4Fe-4S] cluster"
/ligand_id="ChEBI:CHEBI:47739"		 H 3
BINDING 278 278 /ligand="[Ni-4Fe-4S] cluster"
/ligand_id="ChEBI:CHEBI:47739"		 C 3
BINDING 323 323 /ligand="[Ni-4Fe-4S] cluster"
/ligand_id="ChEBI:CHEBI:47739"		 C 3
BINDING 417 417 /ligand="[4Fe-4S] cluster"
/ligand_id="ChEBI:CHEBI:49883"
/ligand_label="3"		 C 4
BINDING 420 420 /ligand="[4Fe-4S] cluster"
/ligand_id="ChEBI:CHEBI:49883"
/ligand_label="3"		 C 4
BINDING 423 423 /ligand="[4Fe-4S] cluster"
/ligand_id="ChEBI:CHEBI:49883"
/ligand_label="3"		 C 4
BINDING 427 427 /ligand="[4Fe-4S] cluster"
/ligand_id="ChEBI:CHEBI:49883"
/ligand_label="4"		 C 5
BINDING 455 455 /ligand="[4Fe-4S] cluster"
/ligand_id="ChEBI:CHEBI:49883"
/ligand_label="4"		 C 5
BINDING 458 458 /ligand="[4Fe-4S] cluster"
/ligand_id="ChEBI:CHEBI:49883"
/ligand_label="4"		 C 5
BINDING 461 461 /ligand="[4Fe-4S] cluster"
/ligand_id="ChEBI:CHEBI:49883"
/ligand_label="4"		 C 5
BINDING 465 465 /ligand="[4Fe-4S] cluster"
/ligand_id="ChEBI:CHEBI:49883"
/ligand_label="3"		 C 4
BINDING 523 523 /ligand="[Ni-4Fe-4S] cluster"
/ligand_id="ChEBI:CHEBI:47739"		 C 3
BINDING 552 552 /ligand="[Ni-4Fe-4S] cluster"
/ligand_id="ChEBI:CHEBI:47739"		 C 3
BINDING 587 587 /ligand="[Ni-4Fe-4S] cluster"
/ligand_id="ChEBI:CHEBI:47739"		 C 3
BINDING 117 117 /ligand="CO"
/ligand_id="ChEBI:CHEBI:17245"		 H

Additional information [?]

Size range 632-807 amino acids
Related rules None
Fusion Nter: None Cter: None



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