HAMAP rule MF_01208
General rule information
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| Accession | MF_01208 |
| Dates | 30-MAY-2002 (Created)
1-JUN-2023 (Last updated, Version 37) |
| Name | PyrE |
| Scope(s) |
Bacteria Archaea |
| Template(s) | P0A7E3 (PYRE_ECOLI); P08870 (PYRE_SALTY); P42719 (PYRE_RHILT); P61498 (PYRE_THET2); P46534 (PYRE_BACCL); [ Recover all ] |
| Triggered by |
HAMAP; MF_01208 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | PYRE |
| Protein name | RecName: Full=Orotate phosphoribosyltransferase; Short=OPRT; Short=OPRTase; EC=2.4.2.10; |
| Gene name | Name=pyrE; |
Comments
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| FUNCTION | Catalyzes the transfer of a ribosyl phosphate group from 5- phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP). |
| CATALYTIC ACTIVITY | Reaction=diphosphate + orotidine 5'-phosphate = 5-phospho-alpha-D- ribose 1-diphosphate + orotate; Xref=Rhea:RHEA:10380, ChEBI:CHEBI:30839, ChEBI:CHEBI:33019, ChEBI:CHEBI:57538, ChEBI:CHEBI:58017; EC=2.4.2.10; |
| COFACTOR | Name=Mg(2+); Xref=ChEBI:CHEBI:18420; |
| PATHWAY | Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from orotate: step 1/2. |
| SUBUNIT | Homodimer. |
| SIMILARITY | Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily. |
Keywords
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Gene Ontology
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| GO:0000287; Molecular function:magnesium ion binding |
| GO:0004588; Molecular function:orotate phosphoribosyltransferase activity |
| GO:0006221; Biological process:pyrimidine nucleotide biosynthetic process |
Cross-references
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| Pfam | PF00156; Pribosyltran; 1; |
| NCBIfam | TIGR00336; PyrE; 1; |
| NCBIfam | TIGR01367; PyrE_Therm; 1; |
| PROSITE | PS00103; PUR_PYR_PR_TRANSFER; 1; |
Features
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| From: PYRE_SALTY (P08870) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| case <OC:Bacteria> | ||||||||||||
| BINDING | 34 | 35 | /ligand="orotate" /ligand_id="ChEBI:CHEBI:30839" |
F-F | ||||||||
| BINDING | 72 | 73 | /ligand="5-phospho-alpha-D-ribose 1-diphosphate" /ligand_id="ChEBI:CHEBI:58017" /ligand_note="ligand shared between dimeric partners" /note="in other chain" |
Y-K | ||||||||
| BINDING | 124 | 132 | /ligand="5-phospho-alpha-D-ribose 1-diphosphate" /ligand_id="ChEBI:CHEBI:58017" /ligand_note="ligand shared between dimeric partners" /note="in other chain" |
|||||||||
| BINDING | 26 | 26 | /ligand="5-phospho-alpha-D-ribose 1-diphosphate" /ligand_id="ChEBI:CHEBI:58017" /ligand_note="ligand shared between dimeric partners" /note="in other chain" |
[KR] | ||||||||
| BINDING | 99 | 99 | /ligand="5-phospho-alpha-D-ribose 1-diphosphate" /ligand_id="ChEBI:CHEBI:58017" /ligand_note="ligand shared between dimeric partners" |
R | ||||||||
| BINDING | 100 | 100 | /ligand="5-phospho-alpha-D-ribose 1-diphosphate" /ligand_id="ChEBI:CHEBI:58017" /ligand_note="ligand shared between dimeric partners" /note="in other chain" |
K | ||||||||
| BINDING | 103 | 103 | /ligand="5-phospho-alpha-D-ribose 1-diphosphate" /ligand_id="ChEBI:CHEBI:58017" /ligand_note="ligand shared between dimeric partners" |
K | ||||||||
| BINDING | 105 | 105 | /ligand="5-phospho-alpha-D-ribose 1-diphosphate" /ligand_id="ChEBI:CHEBI:58017" /ligand_note="ligand shared between dimeric partners" |
H | ||||||||
| BINDING | 128 | 128 | /ligand="orotate" /ligand_id="ChEBI:CHEBI:30839" |
[ST] | ||||||||
| BINDING | 156 | 156 | /ligand="orotate" /ligand_id="ChEBI:CHEBI:30839" |
R | ||||||||
| end case | ||||||||||||
| From: PYRE_PYRHO (O58855) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| case <OC:Archaea> | ||||||||||||
| BINDING | 119 | 127 | /ligand="5-phospho-alpha-D-ribose 1-diphosphate" /ligand_id="ChEBI:CHEBI:58017" /ligand_note="ligand shared between dimeric partners" /note="in other chain" |
|||||||||
| BINDING | 93 | 93 | /ligand="5-phospho-alpha-D-ribose 1-diphosphate" /ligand_id="ChEBI:CHEBI:58017" /ligand_note="ligand shared between dimeric partners" |
R | ||||||||
| BINDING | 94 | 94 | /ligand="5-phospho-alpha-D-ribose 1-diphosphate" /ligand_id="ChEBI:CHEBI:58017" /ligand_note="ligand shared between dimeric partners" /note="in other chain" |
K | ||||||||
| BINDING | 97 | 97 | /ligand="5-phospho-alpha-D-ribose 1-diphosphate" /ligand_id="ChEBI:CHEBI:58017" /ligand_note="ligand shared between dimeric partners" |
[KR] | ||||||||
| BINDING | 99 | 99 | /ligand="5-phospho-alpha-D-ribose 1-diphosphate" /ligand_id="ChEBI:CHEBI:58017" /ligand_note="ligand shared between dimeric partners" |
H | ||||||||
| BINDING | 123 | 123 | /ligand="orotate" /ligand_id="ChEBI:CHEBI:30839" |
T | ||||||||
| BINDING | 151 | 151 | /ligand="orotate" /ligand_id="ChEBI:CHEBI:30839" |
R | ||||||||
| end case | ||||||||||||
Additional information
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| Size range | 166-237 amino acids |
| Related rules |
MF_01214 |
| Fusion | Nter: MF_01200 (pyrF) Cter: None |