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Annotation rule MF_01208
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General rule information [?]

Accession MF_01208
Dates 30-MAY-2002 (Created)
19-NOV-2019 (Last updated, Version 32)
Name PyrE
Scope
Bacteria
Archaea
Templates P0A7E3 (PYRE_ECOLI); P08870 (PYRE_SALTY); P42719 (PYRE_RHILT); P61498 (PYRE_THET2); P46534 (PYRE_BACCL): [Recover all]

Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
PYRE
Protein name
RecName: Full=Orotate phosphoribosyltransferase;
Short=OPRT;
Short=OPRTase;
EC 2.4.2.10;
Gene name
pyrE

Comments [?]

Function Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP).
Catalytic activity RHEA:10380: diphosphate + orotidine 5'-phosphate = 5-phospho-alpha-D-ribose 1-diphosphate + orotate
EC 2.4.2.10
Cofactor Mg(2+)
Pathway Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from orotate: step 1/2.
Subunit Homodimer.
Similarity Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily.

Keywords [?]


Gene Ontology [?]

GO:0000287; Molecular function: magnesium ion binding.
GO:0004588; Molecular function: orotate phosphoribosyltransferase activity.
GO:0006221; Biological process: pyrimidine nucleotide biosynthetic process.

Cross-references [?]

Pfam PF00156; Pribosyltran; 1;
TIGRFAMs TIGR00336; PyrE; 1;
TIGR01367; PyrE_Therm; 1;
PROSITE PS00103; PUR_PYR_PR_TRANSFER; 1;

Features [?]

case <OC:Bacteria>
From: PYRE_SALTY (P08870)
Key     From     To       Description   Tag   Condition   FTGroup
REGION (Optional)     34     35       Orotate binding     F-F  
REGION (Optional)     72     73       5-phosphoribose 1-diphosphate binding     Y-K  
REGION     124     132       5-phosphoribose 1-diphosphate binding        
BINDING (Optional)     26     26       5-phosphoribose 1-diphosphate     [KR]  
BINDING (Optional)     99     99       5-phosphoribose 1-diphosphate; shared with dimeric partner     R  
BINDING (Optional)     100     100       5-phosphoribose 1-diphosphate     K  
BINDING (Optional)     103     103       5-phosphoribose 1-diphosphate; shared with dimeric partner     K  
BINDING (Optional)     105     105       5-phosphoribose 1-diphosphate; shared with dimeric partner     H  
BINDING (Optional)     128     128       Orotate     [ST]  
BINDING (Optional)     156     156       Orotate     R  
end case
case <OC:Archaea>
From: PYRE_PYRHO (O58855)
REGION     119     127       5-phosphoribose 1-diphosphate binding        
BINDING (Optional)     93     93       5-phosphoribose 1-diphosphate; shared with dimeric partner     R  
BINDING (Optional)     94     94       5-phosphoribose 1-diphosphate     K  
BINDING (Optional)     97     97       5-phosphoribose 1-diphosphate; shared with dimeric partner     [KR]  
BINDING (Optional)     99     99       5-phosphoribose 1-diphosphate; shared with dimeric partner     H  
BINDING (Optional)     123     123       Orotate     T  
BINDING (Optional)     151     151       Orotate     R  
end case

Additional information [?]

Size range 166-237 amino acids
Related rules MF_01214 (PYREL)
Fusion Nter: MF_01200 (pyrF); Cter: None