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HAMAP rule MF_01208

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General rule information [?]

Accession MF_01208
Dates 30-MAY-2002 (Created)
14-MAY-2024 (Last updated, Version 38)
Name PyrE
Scope(s) Bacteria
Archaea
Template(s) P0A7E3 (PYRE_ECOLI); P08870 (PYRE_SALTY); P42719 (PYRE_RHILT); P61498 (PYRE_THET2); P46534 (PYRE_BACCL); [ Recover all ]
Triggered by HAMAP; MF_01208 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier PYRE
Protein name RecName: Full=Orotate phosphoribosyltransferase;
                 Short=OPRT;
                 Short=OPRTase;
                 EC=2.4.2.10;
Gene name Name=pyrE;

Comments [?]

FUNCTIONCatalyzes the transfer of a ribosyl phosphate group from 5- phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP).
CATALYTIC ACTIVITY Reaction=diphosphate + orotidine 5'-phosphate = 5-phospho-alpha-D- ribose 1-diphosphate + orotate; Xref=Rhea:RHEA:10380, ChEBI:CHEBI:30839, ChEBI:CHEBI:33019, ChEBI:CHEBI:57538, ChEBI:CHEBI:58017; EC=2.4.2.10;
COFACTOR Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
PATHWAYPyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from orotate: step 1/2.
SUBUNITHomodimer.
SIMILARITYBelongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily.

Keywords [?]

Pyrimidine biosynthesis
Transferase
Glycosyltransferase
Magnesium

Gene Ontology [?]

GO:0000287; Molecular function:magnesium ion binding
GO:0004588; Molecular function:orotate phosphoribosyltransferase activity
GO:0044205; Biological process:'de novo' UMP biosynthetic process

Cross-references [?]

Pfam PF00156; Pribosyltran; 1;
NCBIfam TIGR00336; PyrE; 1;
NCBIfam TIGR01367; PyrE_Therm; 1;
PROSITE PS00103; PUR_PYR_PR_TRANSFER; 1;

Features [?]

From: PYRE_SALTY (P08870)
Key From To Description Tag Condition FTGroup
case <OC:Bacteria>
BINDING 34 35 /ligand="orotate"
/ligand_id="ChEBI:CHEBI:30839"		 F-F
BINDING 72 73 /ligand="5-phospho-alpha-D-ribose 1-diphosphate"
/ligand_id="ChEBI:CHEBI:58017"
/ligand_note="ligand shared between dimeric partners"
/note="in other chain"		 Y-K
BINDING 124 132 /ligand="5-phospho-alpha-D-ribose 1-diphosphate"
/ligand_id="ChEBI:CHEBI:58017"
/ligand_note="ligand shared between dimeric partners"
/note="in other chain"
BINDING 26 26 /ligand="5-phospho-alpha-D-ribose 1-diphosphate"
/ligand_id="ChEBI:CHEBI:58017"
/ligand_note="ligand shared between dimeric partners"
/note="in other chain"		 [KR]
BINDING 99 99 /ligand="5-phospho-alpha-D-ribose 1-diphosphate"
/ligand_id="ChEBI:CHEBI:58017"
/ligand_note="ligand shared between dimeric partners"		 R
BINDING 100 100 /ligand="5-phospho-alpha-D-ribose 1-diphosphate"
/ligand_id="ChEBI:CHEBI:58017"
/ligand_note="ligand shared between dimeric partners"
/note="in other chain"		 K
BINDING 103 103 /ligand="5-phospho-alpha-D-ribose 1-diphosphate"
/ligand_id="ChEBI:CHEBI:58017"
/ligand_note="ligand shared between dimeric partners"		 K
BINDING 105 105 /ligand="5-phospho-alpha-D-ribose 1-diphosphate"
/ligand_id="ChEBI:CHEBI:58017"
/ligand_note="ligand shared between dimeric partners"		 H
BINDING 128 128 /ligand="orotate"
/ligand_id="ChEBI:CHEBI:30839"		 [ST]
BINDING 156 156 /ligand="orotate"
/ligand_id="ChEBI:CHEBI:30839"		 R
end case
From: PYRE_PYRHO (O58855)
Key From To Description Tag Condition FTGroup
case <OC:Archaea>
BINDING 119 127 /ligand="5-phospho-alpha-D-ribose 1-diphosphate"
/ligand_id="ChEBI:CHEBI:58017"
/ligand_note="ligand shared between dimeric partners"
/note="in other chain"
BINDING 93 93 /ligand="5-phospho-alpha-D-ribose 1-diphosphate"
/ligand_id="ChEBI:CHEBI:58017"
/ligand_note="ligand shared between dimeric partners"		 R
BINDING 94 94 /ligand="5-phospho-alpha-D-ribose 1-diphosphate"
/ligand_id="ChEBI:CHEBI:58017"
/ligand_note="ligand shared between dimeric partners"
/note="in other chain"		 K
BINDING 97 97 /ligand="5-phospho-alpha-D-ribose 1-diphosphate"
/ligand_id="ChEBI:CHEBI:58017"
/ligand_note="ligand shared between dimeric partners"		 [KR]
BINDING 99 99 /ligand="5-phospho-alpha-D-ribose 1-diphosphate"
/ligand_id="ChEBI:CHEBI:58017"
/ligand_note="ligand shared between dimeric partners"		 H
BINDING 123 123 /ligand="orotate"
/ligand_id="ChEBI:CHEBI:30839"		 T
BINDING 151 151 /ligand="orotate"
/ligand_id="ChEBI:CHEBI:30839"		 R
end case

Additional information [?]

Size range 166-237 amino acids
Related rules MF_01214
Fusion Nter: MF_01200 (pyrF) Cter: None



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