HAMAP rule MF_01209

General rule information [?]

Accession	MF_01209
Dates	4-JUL-2002 (Created) 3-SEP-2024 (Last updated, Version 50)
Name	CPSase_S_chain
Scope(s)	Bacteria Archaea Plastid
Template(s)	P0A6F1 (CARA_ECOLI); P54324 (CARX_GEOSE); Q9L4N5 (CARA_LACLM); P77885 (CARA_LACPL); O08317 (CARX_LACPL); [ Recover all ]
Triggered by	HAMAP; MF_01209 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier	CARA
Protein name	RecName: Full=Carbamoyl phosphate synthase small chain; EC=6.3.5.5; AltName: Full=Carbamoyl phosphate synthetase glutamine chain;
Gene name	Name=carA;

Comments [?]

FUNCTION	Small subunit of the glutamine-dependent carbamoyl phosphate synthetase (CPSase). CPSase catalyzes the formation of carbamoyl phosphate from the ammonia moiety of glutamine, carbonate, and phosphate donated by ATP, constituting the first step of 2 biosynthetic pathways, one leading to arginine and/or urea and the other to pyrimidine nucleotides. The small subunit (glutamine amidotransferase) binds and cleaves glutamine to supply the large subunit with the substrate ammonia.
CATALYTIC ACTIVITY	Reaction=hydrogencarbonate + L-glutamine + 2 ATP + H2O = carbamoyl phosphate + L-glutamate + 2 ADP + phosphate + 2 H(+); Xref=Rhea:RHEA:18633, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17544, ChEBI:CHEBI:29985, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474, ChEBI:CHEBI:58228, ChEBI:CHEBI:58359, ChEBI:CHEBI:456216; EC=6.3.5.5;
CATALYTIC ACTIVITY	[]: Reaction=L-glutamine + H2O = L-glutamate + NH4(+); Xref=Rhea:RHEA:15889, ChEBI:CHEBI:15377, ChEBI:CHEBI:28938, ChEBI:CHEBI:29985, ChEBI:CHEBI:58359;
PATHWAY	Amino-acid biosynthesis; L-arginine biosynthesis; carbamoyl phosphate from bicarbonate: step 1/1.
PATHWAY	Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 1/3.
SUBUNIT	Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate. Tetramer of heterodimers (alpha,beta)4.
case <OG:Chloroplast>
SUBCELLULAR LOCATION	Plastid, chloroplast.
end case
SIMILARITY	Belongs to the CarA family.

Keywords [?]

Amino-acid biosynthesis

Arginine biosynthesis

ATP-binding

Glutamine amidotransferase

Ligase

Nucleotide-binding

Pyrimidine biosynthesis

Gene Ontology [?]

GO:0005524; Molecular function:ATP binding
GO:0004088; Molecular function:carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity
GO:0006526; Biological process:arginine biosynthetic process
GO:0044205; Biological process:'de novo' UMP biosynthetic process
case <OG:Chloroplast>
GO:0009507; Cellular component:chloroplast
end case

Cross-references [?]

Pfam	PF00117; GATase; 1;
Pfam	PF00988; CPSase_sm_chain; 1;
PRINTS	PR00099; CPSGATASE; 1;
PRINTS	PR00096; GATASE; 1;
PROSITE	PS51273; GATASE_TYPE_1; 1;
NCBIfam	TIGR01368; CPSaseIIsmall; 1;

Features [?]

From: CARA_ECOLI (P0A6F1)

Key

From

Description

Tag

Condition

FTGroup

REGION

Nter

189

/note="CPSase"

ACT_SITE

269

/note="Nucleophile"

ACT_SITE

353

ACT_SITE

355

[ED]

BINDING