HAMAP rule MF_01209
General rule information
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| Accession | MF_01209 |
| Dates | 4-JUL-2002 (Created) 1-JUN-2023 (Last updated, Version 47) |
| Name | CPSase_S_chain |
| Scope | Bacteria
Archaea
Plastid |
| Templates | P0A6F1 (CARA_ECOLI); P54324 (CARX_GEOSE); Q9L4N5 (CARA_LACLM); P77885 (CARA_LACPL); O08317 (CARX_LACPL): [Recover all] |
| Triggered by |
Propagated annotation
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Identifier, protein and gene names
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| Identifier |
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| Protein name |
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| Gene name |
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Comments
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| Catalytic activity | RHEA:18633: 2 ATP + H2O + hydrogencarbonate + L-glutamine = 2 ADP + carbamoyl phosphate + 2 H(+) + L-glutamate + phosphate
EC 6.3.5.5 |
| Pathway | Amino-acid biosynthesis; L-arginine biosynthesis; carbamoyl phosphate from bicarbonate: step 1/1. |
| Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 1/3. | |
| Subunit | Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate. |
case <OG:Chloroplast>
| Subcellular location | Plastid, chloroplast. |
end case
| Similarity | Belongs to the CarA family. |
Keywords
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Amino-acid biosynthesis
Arginine biosynthesis
ATP-binding
Glutamine amidotransferase
Ligase
Nucleotide-binding
Pyrimidine biosynthesis
Arginine biosynthesis
ATP-binding
Glutamine amidotransferase
Ligase
Nucleotide-binding
Pyrimidine biosynthesis
Gene Ontology
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GO:0005524; Molecular function: ATP binding.
GO:0004088; Molecular function: carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity.
GO:0006526; Biological process: arginine biosynthetic process.
GO:0006221; Biological process: pyrimidine nucleotide biosynthetic process.
GO:0004088; Molecular function: carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity.
GO:0006526; Biological process: arginine biosynthetic process.
GO:0006221; Biological process: pyrimidine nucleotide biosynthetic process.
case <OG:Chloroplast>
GO:0009507; Cellular component: chloroplast.
end case
Cross-references
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| Pfam | PF00117; GATase; 1; |
| PF00988; CPSase_sm_chain; 1; | |
| PRINTS | PR00099; CPSGATASE; 1; |
| PR00096; GATASE; 1; | |
| PROSITE | PS51273; GATASE_TYPE_1; 1; trigger=PRU00605; |
| NCBIfam | TIGR01368; CPSaseIIsmall; 1; |
Features
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| From: CARA_ECOLI (P0A6F1) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| REGION | Nter | 189 | CPSase | |||||||||
| ACT_SITE | 269 | 269 | Nucleophile | C | ||||||||
| ACT_SITE | 353 | 353 | H | |||||||||
| ACT_SITE | 355 | 355 | [ED] | |||||||||
Additional information
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| Size range | 345-407 amino acids |
| Related rules | None |
| Fusion | None |
| Comments | Bacillaceae, Geobacillus and lactobacillaceae seem to posess two members of this family: one specific for arginine biosynthesis (called CARX_ in ID, and CARA in GN) and the other specific for pyrimidine synthesis (called CARA_ in ID, and PYRAA in GN). Weird insert in THEMA; not shown in alignment. |