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Annotation rule MF_01209
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General rule information [?]

Accession MF_01209
Dates 4-JUL-2002 (Created)
19-NOV-2019 (Last updated, Version 44)
Name CPSase_S_chain
Scope
Bacteria
Archaea
Plastid
Templates P0A6F1 (CARA_ECOLI); P54324 (CARX_GEOSE); Q9L4N5 (CARA_LACLM); P77885 (CARA_LACPL); O08317 (CARX_LACPL): [Recover all]

Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
CARA
Protein name
RecName: Full=Carbamoyl-phosphate synthase small chain;
EC 6.3.5.5;
AltName: Full=Carbamoyl-phosphate synthetase glutamine chain;
Gene name
carA

Comments [?]

Catalytic activity RHEA:18633: 2 ATP + H2O + hydrogencarbonate + L-glutamine = 2 ADP + carbamoyl phosphate + 2 H(+) + L-glutamate + phosphate
EC 6.3.5.5
Pathway Amino-acid biosynthesis; L-arginine biosynthesis; carbamoyl phosphate from bicarbonate: step 1/1.
Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 1/3.
Subunit Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate.
case <OG:Chloroplast>
Subcellular location Plastid, chloroplast.
end case
Similarity Belongs to the CarA family.

Keywords [?]


Gene Ontology [?]

GO:0005524; Molecular function: ATP binding.
GO:0004088; Molecular function: carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity.
GO:0006526; Biological process: arginine biosynthetic process.
GO:0006221; Biological process: pyrimidine nucleotide biosynthetic process.
case <OG:Chloroplast>
GO:0009507; Cellular component: chloroplast.
end case

Cross-references [?]

Pfam PF00117; GATase; 1;
PF00988; CPSase_sm_chain; 1;
PRINTS PR00099; CPSGATASE; 1;
PR00096; GATASE; 1;
PROSITE PS51273; GATASE_TYPE_1; 1; trigger=PRU00605;
TIGRFAMs TIGR01368; CPSaseIIsmall; 1;

Features [?]

From: CARA_ECOLI (P0A6F1)
Key     From     To       Description   Tag   Condition   FTGroup
REGION     Nter     189       CPSase        
ACT_SITE     269     269       Nucleophile     C  
ACT_SITE     353     353             H  
ACT_SITE     355     355             [ED]  

Additional information [?]

Size range 345-407 amino acids
Related rules None
Fusion None
Comments Bacillaceae, Geobacillus and lactobacillaceae seem to posess two members of this family: one specific for arginine biosynthesis (called CARX_ in ID, and CARA in GN) and the other specific for pyrimidine synthesis (called CARA_ in ID, and PYRAA in GN). Weird insert in THEMA; not shown in alignment.