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HAMAP rule MF_01209

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General rule information [?]

Accession MF_01209
Dates 4-JUL-2002 (Created)
14-MAY-2024 (Last updated, Version 49)
Name CPSase_S_chain
Scope(s) Bacteria
Archaea
Plastid
Template(s) P0A6F1 (CARA_ECOLI); P54324 (CARX_GEOSE); Q9L4N5 (CARA_LACLM); P77885 (CARA_LACPL); O08317 (CARX_LACPL); [ Recover all ]
Triggered by HAMAP; MF_01209 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier CARA
Protein name RecName: Full=Carbamoyl phosphate synthase small chain;
                 EC=6.3.5.5;
AltName: Full=Carbamoyl phosphate synthetase glutamine chain;
Gene name Name=carA;

Comments [?]

FUNCTIONSmall subunit of the glutamine-dependent carbamoyl phosphate synthetase (CPSase). CPSase catalyzes the formation of carbamoyl phosphate from the ammonia moiety of glutamine, carbonate, and phosphate donated by ATP, constituting the first step of 2 biosynthetic pathways, one leading to arginine and/or urea and the other to pyrimidine nucleotides. The small subunit (glutamine amidotransferase) binds and cleaves glutamine to supply the large subunit with the substrate ammonia.
CATALYTIC ACTIVITY Reaction=2 ATP + H2O + hydrogencarbonate + L-glutamine = 2 ADP + carbamoyl phosphate + 2 H(+) + L-glutamate + phosphate; Xref=Rhea:RHEA:18633, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17544, ChEBI:CHEBI:29985, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474, ChEBI:CHEBI:58228, ChEBI:CHEBI:58359, ChEBI:CHEBI:456216; EC=6.3.5.5;
CATALYTIC ACTIVITY[]: Reaction=H2O + L-glutamine = L-glutamate + NH4(+); Xref=Rhea:RHEA:15889, ChEBI:CHEBI:15377, ChEBI:CHEBI:28938, ChEBI:CHEBI:29985, ChEBI:CHEBI:58359;
PATHWAYAmino-acid biosynthesis; L-arginine biosynthesis; carbamoyl phosphate from bicarbonate: step 1/1.
PATHWAYPyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 1/3.
SUBUNITComposed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate. Tetramer of heterodimers (alpha,beta)4.
case <OG:Chloroplast>
SUBCELLULAR LOCATIONPlastid, chloroplast.
end case
SIMILARITYBelongs to the CarA family.

Keywords [?]


Gene Ontology [?]

GO:0005524; Molecular function:ATP binding
GO:0004088; Molecular function:carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity
GO:0006526; Biological process:arginine biosynthetic process
GO:0044205; Biological process:'de novo' UMP biosynthetic process
case <OG:Chloroplast>
GO:0009507; Cellular component:chloroplast
end case

Cross-references [?]

Pfam PF00117; GATase; 1;
Pfam PF00988; CPSase_sm_chain; 1;
PRINTS PR00099; CPSGATASE; 1;
PRINTS PR00096; GATASE; 1;
PROSITE PS51273; GATASE_TYPE_1; 1;
NCBIfam TIGR01368; CPSaseIIsmall; 1;

Features [?]

From: CARA_ECOLI (P0A6F1)
Key From To Description Tag Condition FTGroup
REGION Nter 189 /note="CPSase"
ACT_SITE 269 269 /note="Nucleophile" C
ACT_SITE 353 353 H
ACT_SITE 355 355 [ED]
BINDING 47 47 /ligand="L-glutamine"
/ligand_id="ChEBI:CHEBI:58359"
S
BINDING 241 241 /ligand="L-glutamine"
/ligand_id="ChEBI:CHEBI:58359"
G
BINDING 243 243 /ligand="L-glutamine"
/ligand_id="ChEBI:CHEBI:58359"
G
BINDING 270 270 /ligand="L-glutamine"
/ligand_id="ChEBI:CHEBI:58359"
[LFM]
BINDING 273 273 /ligand="L-glutamine"
/ligand_id="ChEBI:CHEBI:58359"
Q
BINDING 311 311 /ligand="L-glutamine"
/ligand_id="ChEBI:CHEBI:58359"
N
BINDING 313 313 /ligand="L-glutamine"
/ligand_id="ChEBI:CHEBI:58359"
G
BINDING 314 314 /ligand="L-glutamine"
/ligand_id="ChEBI:CHEBI:58359"
[YF]

Additional information [?]

Size range 345-407 amino acids
Related rules None
Fusion Nter: None Cter: None
Comments Bacillaceae, Geobacillus and lactobacillaceae seem to posess two members of this family: one specific for arginine biosynthesis (called CARX_ in ID, and CARA in GN) and the other specific for pyrimidine synthesis (called CARA_ in ID, and PYRAA in GN). Weird insert in THEMA; not shown in alignment.



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