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HAMAP rule MF_01225

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General rule information [?]

PURL https://purl.expasy.org/hamap/rule/MF_01225
Accession MF_01225
Dates 28-FEB-2005 (Created)
03-SEP-2024 (Last updated, Version 30)
Name MoaA
Scope(s) Bacteria
Archaea
Template(s) P69848; Q44118; P30745; Q9X5W3; [ Recover all ]
Triggered by
case c? <OC:Bacteria>
HAMAP; MF_01225_B (Get profile general information and statistics)
end case
case c? <OC:Archaea>
HAMAP; MF_01225_A (Get profile general information and statistics)
end case

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier MOAA
case <OC:Bacteria>
Protein name RecName: Full=GTP 3',8-cyclase;
                 EC=4.1.99.22;
AltName: Full=Molybdenum cofactor biosynthesis protein A;
end case
case <OC:Archaea>
Protein name RecName: Full=Probable GTP 3',8-cyclase;
                 EC=4.1.99.22;
AltName: Full=Molybdenum cofactor biosynthesis protein A;
end case
Gene name Name=moaA;

Comments [?]

FUNCTIONCatalyzes the cyclization of GTP to (8S)-3',8-cyclo-7,8- dihydroguanosine 5'-triphosphate.
CATALYTIC ACTIVITY Reaction=GTP + AH2 + S-adenosyl-L-methionine = (8S)-3',8-cyclo-7,8- dihydroguanosine 5'-triphosphate + 5'-deoxyadenosine + L-methionine + A + H(+); Xref=Rhea:RHEA:49576, ChEBI:CHEBI:13193, ChEBI:CHEBI:15378, ChEBI:CHEBI:17319, ChEBI:CHEBI:17499, ChEBI:CHEBI:37565, ChEBI:CHEBI:57844, ChEBI:CHEBI:59789, ChEBI:CHEBI:131766; EC=4.1.99.22;
COFACTOR Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883; Note=Binds 2 [4Fe-4S] clusters. Binds 1 [4Fe-4S] cluster coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine and 1 [4Fe-4S] cluster coordinated with 3 cysteines and the GTP-derived substrate.;
PATHWAYCofactor biosynthesis; molybdopterin biosynthesis.
case <OC:Bacteria>
SUBUNITMonomer and homodimer.
end case
SIMILARITYBelongs to the radical SAM superfamily. MoaA family.

Keywords [?]

4Fe-4S
GTP-binding
Iron
Iron-sulfur
Lyase
Metal-binding
Molybdenum cofactor biosynthesis
Nucleotide-binding
S-adenosyl-L-methionine

Gene Ontology [?]

GO:0005525; Molecular function:GTP binding
GO:0051539; Molecular function:4 iron, 4 sulfur cluster binding
GO:0061798; Molecular function:GTP 3',8'-cyclase activity
GO:1904047; Molecular function:S-adenosyl-L-methionine binding
GO:0006777; Biological process:Mo-molybdopterin cofactor biosynthetic process

Cross-references [?]

PROSITE PS01305; MOAA_NIFB_PQQE; 1;
Pfam PF06463; Mob_synth_C; 1;
Pfam PF04055; Radical_SAM; 1;
NCBIfam TIGR02666; MoaA; 1;
NCBIfam TIGR02668; moaA_archaeal; 1;

Features [?]

From: MOAA_STAA8 (P69848)
Key From To Description Tag Condition FTGroup
case <OC:Bacteria>
BINDING 266 268 /ligand="GTP"
/ligand_id="ChEBI:CHEBI:37565"		 [RK]-x-R
BINDING 24 24 /ligand="[4Fe-4S] cluster"
/ligand_id="ChEBI:CHEBI:49883"
/ligand_label="1"
/ligand_note="4Fe-4S-S-AdoMet"		 C
BINDING 28 28 /ligand="[4Fe-4S] cluster"
/ligand_id="ChEBI:CHEBI:49883"
/ligand_label="1"
/ligand_note="4Fe-4S-S-AdoMet"		 C
BINDING 31 31 /ligand="[4Fe-4S] cluster"
/ligand_id="ChEBI:CHEBI:49883"
/ligand_label="1"
/ligand_note="4Fe-4S-S-AdoMet"		 C
BINDING 261 261 /ligand="[4Fe-4S] cluster"
/ligand_id="ChEBI:CHEBI:49883"
/ligand_label="2"
/ligand_note="4Fe-4S-substrate"		 C
BINDING 264 264 /ligand="[4Fe-4S] cluster"
/ligand_id="ChEBI:CHEBI:49883"
/ligand_label="2"
/ligand_note="4Fe-4S-substrate"		 C
BINDING 278 278 /ligand="[4Fe-4S] cluster"
/ligand_id="ChEBI:CHEBI:49883"
/ligand_label="2"
/ligand_note="4Fe-4S-substrate"		 C
BINDING 17 17 /ligand="GTP"
/ligand_id="ChEBI:CHEBI:37565"		 R
BINDING 30 30 /ligand="S-adenosyl-L-methionine"
/ligand_id="ChEBI:CHEBI:59789"		 Y
BINDING 71 71 /ligand="GTP"
/ligand_id="ChEBI:CHEBI:37565"		 R
BINDING 75 75 /ligand="S-adenosyl-L-methionine"
/ligand_id="ChEBI:CHEBI:59789"		 G
BINDING 102 102 /ligand="GTP"
/ligand_id="ChEBI:CHEBI:37565"		 [TS]
BINDING 126 126 /ligand="S-adenosyl-L-methionine"
/ligand_id="ChEBI:CHEBI:59789"		 S
BINDING 163 163 /ligand="GTP"
/ligand_id="ChEBI:CHEBI:37565"		 K
BINDING 197 197 /ligand="S-adenosyl-L-methionine"
/ligand_id="ChEBI:CHEBI:59789"		 M
end case
From: MOAA_PYRAB (Q9V2G2)
Key From To Description Tag Condition FTGroup
case <OC:Archaea>
BINDING 255 257 /ligand="GTP"
/ligand_id="ChEBI:CHEBI:37565"		 R-x-R
BINDING 21 21 /ligand="[4Fe-4S] cluster"
/ligand_id="ChEBI:CHEBI:49883"
/ligand_label="1"
/ligand_note="4Fe-4S-S-AdoMet"		 C
BINDING 25 25 /ligand="[4Fe-4S] cluster"
/ligand_id="ChEBI:CHEBI:49883"
/ligand_label="1"
/ligand_note="4Fe-4S-S-AdoMet"		 C
BINDING 28 28 /ligand="[4Fe-4S] cluster"
/ligand_id="ChEBI:CHEBI:49883"
/ligand_label="1"
/ligand_note="4Fe-4S-S-AdoMet"		 C
BINDING 250 250 /ligand="[4Fe-4S] cluster"
/ligand_id="ChEBI:CHEBI:49883"
/ligand_label="2"
/ligand_note="4Fe-4S-substrate"		 C
BINDING 253 253 /ligand="[4Fe-4S] cluster"
/ligand_id="ChEBI:CHEBI:49883"
/ligand_label="2"
/ligand_note="4Fe-4S-substrate"		 C
BINDING 267 267 /ligand="[4Fe-4S] cluster"
/ligand_id="ChEBI:CHEBI:49883"
/ligand_label="2"
/ligand_note="4Fe-4S-substrate"		 C
BINDING 14 14 /ligand="GTP"
/ligand_id="ChEBI:CHEBI:37565"		 R
BINDING 27 27 /ligand="S-adenosyl-L-methionine"
/ligand_id="ChEBI:CHEBI:59789"		 Y
BINDING 61 61 /ligand="GTP"
/ligand_id="ChEBI:CHEBI:37565"		 [KR]
BINDING 65 65 /ligand="S-adenosyl-L-methionine"
/ligand_id="ChEBI:CHEBI:59789"		 G
BINDING 90 90 /ligand="GTP"
/ligand_id="ChEBI:CHEBI:37565"		 T
BINDING 114 114 /ligand="S-adenosyl-L-methionine"
/ligand_id="ChEBI:CHEBI:59789"		 S
BINDING 150 150 /ligand="GTP"
/ligand_id="ChEBI:CHEBI:37565"		 K
BINDING 189 189 /ligand="S-adenosyl-L-methionine"
/ligand_id="ChEBI:CHEBI:59789"		 M
end case

Additional information [?]

Size range 296-357 amino acids
Related rules None
Fusion Nter: None Cter: None



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