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Annotation rule MF_01225
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General rule information [?]

Accession MF_01225
Dates 13-SEP-2002 (Created)
19-NOV-2019 (Last updated, Version 31)
Name MoaA
Scope
Bacteria
Archaea
Templates P69848 (MOAA_STAA8); Q44118 (MOAA_PAENI); P30745 (MOAA_ECOLI); Q9X5W3 (MOAA_RHOCA): [Recover all]
case <OC:Bacteria>
Triggered by
end case

case <OC:Archaea>
Triggered by
end case


Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
MOAA
case <OC:Bacteria>
Protein name
RecName: Full=GTP 3',8-cyclase;
EC 4.1.99.22;
AltName: Full=Molybdenum cofactor biosynthesis protein A;
end case
case <OC:Archaea>
Protein name
RecName: Full=Probable GTP 3',8-cyclase;
EC 4.1.99.22;
AltName: Full=Molybdenum cofactor biosynthesis protein A;
end case
Gene name
moaA

Comments [?]

Function Catalyzes the cyclization of GTP to (8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate.
Catalytic activity RHEA:49576: AH2 + GTP + S-adenosyl-L-methionine = (8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate + 5'-deoxyadenosine + A + H(+) + L-methionine
EC 4.1.99.22
Cofactor [4Fe-4S] cluster
Note: Binds 2 [4Fe-4S] clusters. Binds 1 [4Fe-4S] cluster coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine and 1 [4Fe-4S] cluster coordinated with 3 cysteines and the GTP-derived substrate.
Pathway Cofactor biosynthesis; molybdopterin biosynthesis.
case <OC:Bacteria>
Subunit Monomer and homodimer.
end case
Similarity Belongs to the radical SAM superfamily. MoaA family.

Keywords [?]

4Fe-4S
GTP-binding
Iron
Iron-sulfur
Lyase
Metal-binding
Molybdenum cofactor biosynthesis
Nucleotide-binding
S-adenosyl-L-methionine

Gene Ontology [?]

GO:0005525; Molecular function: GTP binding.
GO:0051539; Molecular function: 4 iron, 4 sulfur cluster binding.
GO:0061798; Molecular function: GTP 3',8'-cyclase activity.
GO:1904047; Molecular function: S-adenosyl-L-methionine binding.
GO:0006777; Biological process: Mo-molybdopterin cofactor biosynthetic process.

Cross-references [?]

PROSITE PS01305; MOAA_NIFB_PQQE; 1;
Pfam PF06463; Mob_synth_C; 1;
PF04055; Radical_SAM; 1;
TIGRFAMs TIGR02666; MoaA; 1;
TIGR02668; moaA_archaeal; 1;

Features [?]

case <OC:Bacteria>
From: MOAA_STAA8 (P69848)
Key     From     To       Description   Tag   Condition   FTGroup
NP_BIND     266     268       GTP     [RK]-x-R  
METAL     24     24       Iron-sulfur 1 (4Fe-4S-S-AdoMet)     C  
METAL     28     28       Iron-sulfur 1 (4Fe-4S-S-AdoMet)     C  
METAL     31     31       Iron-sulfur 1 (4Fe-4S-S-AdoMet)     C  
METAL     261     261       Iron-sulfur 2 (4Fe-4S-substrate)     C  
METAL     264     264       Iron-sulfur 2 (4Fe-4S-substrate)     C  
METAL     278     278       Iron-sulfur 2 (4Fe-4S-substrate)     C  
BINDING     17     17       GTP     R  
BINDING     30     30       S-adenosyl-L-methionine     Y  
BINDING     71     71       GTP     R  
BINDING     75     75       S-adenosyl-L-methionine; via carbonyl oxygen     G  
BINDING     102     102       GTP     [TS]  
BINDING     126     126       S-adenosyl-L-methionine     S  
BINDING     163     163       GTP     K  
BINDING     197     197       S-adenosyl-L-methionine; via amide nitrogen and carbonyl oxygen     M  
end case
case <OC:Archaea>
From: MOAA_PYRAB (Q9V2G2)
NP_BIND     255     257       GTP     R-x-R  
METAL     21     21       Iron-sulfur 1 (4Fe-4S-S-AdoMet)     C  
METAL     25     25       Iron-sulfur 1 (4Fe-4S-S-AdoMet)     C  
METAL     28     28       Iron-sulfur 1 (4Fe-4S-S-AdoMet)     C  
METAL     250     250       Iron-sulfur 2 (4Fe-4S-substrate)     C  
METAL     253     253       Iron-sulfur 2 (4Fe-4S-substrate)     C  
METAL     267     267       Iron-sulfur 2 (4Fe-4S-substrate)     C  
BINDING     14     14       GTP     R  
BINDING (Optional)     27     27       S-adenosyl-L-methionine     Y  
BINDING     61     61       GTP     [KR]  
BINDING     65     65       S-adenosyl-L-methionine; via carbonyl oxygen     G  
BINDING     90     90       GTP     T  
BINDING     114     114       S-adenosyl-L-methionine     S  
BINDING     150     150       GTP     K  
BINDING (Optional)     189     189       S-adenosyl-L-methionine; via amide nitrogen and carbonyl oxygen     M  
end case

Additional information [?]

case <OC:Bacteria>
Size range 318-378 amino acids
end case
case <OC:Archaea>
Size range 296-357 amino acids
end case
Related rules None
Fusion None


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