HAMAP rule MF_01321
General rule information
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| PURL | https://purl.expasy.org/hamap/rule/MF_01321 |
| Accession | MF_01321 |
| Dates | 28-FEB-2005 (Created)
21-MAR-2025 (Last updated, Version 33) |
| Name | RNApol_bact_RpoB |
| Scope(s) |
Bacteria Plastid |
| Template(s) | Q9KWU7; P0A8V2; [ Recover all ] |
| Triggered by |
HAMAP; MF_01321 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | RPOB |
| case not <OG:Chloroplast> | |
| Protein name | RecName: Full=DNA-directed RNA polymerase subunit beta; Short=RNAP subunit beta; EC=2.7.7.6; AltName: Full=RNA polymerase subunit beta; AltName: Full=Transcriptase subunit beta; |
| else case <OG:Chloroplast> | |
| Protein name | RecName: Full=DNA-directed RNA polymerase subunit beta; EC=2.7.7.6; AltName: Full=PEP; AltName: Full=Plastid-encoded RNA polymerase subunit beta; Short=RNA polymerase subunit beta; |
| end case | |
| Gene name | Name=rpoB; |
Comments
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| FUNCTION | DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. |
| CATALYTIC ACTIVITY | Reaction=RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate; Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA- COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395; EC=2.7.7.6; |
| case not <OG:Chloroplast> and not <OC:Cyanobacteriota> | |
| SUBUNIT | The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta' and 1 omega subunit. When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription. |
| else case <OC:Cyanobacteriota> | |
| SUBUNIT | In cyanobacteria the RNAP catalytic core is composed of 2 alpha, 1 beta, 1 beta', 1 gamma and 1 omega subunit. When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription. |
| else case <OG:Chloroplast> | |
| SUBUNIT | In plastids the minimal PEP RNA polymerase catalytic core is composed of four subunits: alpha, beta, beta', and beta''. When a (nuclear-encoded) sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription. |
| SUBCELLULAR LOCATION | Plastid, chloroplast. |
| end case | |
| SIMILARITY | Belongs to the RNA polymerase beta chain family. |
Keywords
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| case (<OC:Escherichia> or <OC:Shigella>) and (<FT:1> or <FT:2>) | |
| Acetylation | |
| end case | |
| Transcription | |
| Transferase | |
| Nucleotidyltransferase | |
| DNA-directed RNA polymerase | |
Gene Ontology
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| GO:0003677; Molecular function:DNA binding | |
| GO:0003899; Molecular function:DNA-directed RNA polymerase activity | |
| GO:0006351; Biological process:DNA-templated transcription | |
| case <OG:Chloroplast> | |
| GO:0009507; Cellular component:chloroplast | |
| end case | |
Cross-references
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| Pfam | PF04563; RNA_pol_Rpb2_1; 1; |
| Pfam | PF04561; RNA_pol_Rpb2_2; 1; |
| Pfam | PF04565; RNA_pol_Rpb2_3; 1; |
| Pfam | PF00562; RNA_pol_Rpb2_6; 1; |
| Pfam | PF04560; RNA_pol_Rpb2_7; 1; |
| NCBIfam | TIGR02013; RpoB; 1; |
| PROSITE | PS01166; RNA_POL_BETA; 1; |
Features
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| From: RPOB_ECOLI (P0A8V2) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| case <OC:Escherichia> or <OC:Shigella> | ||||||||||||
| MOD_RES | 1022 | 1022 | /note="N6-acetyllysine" | K | ||||||||
| MOD_RES | 1200 | 1200 | /note="N6-acetyllysine" | K | ||||||||
| end case | ||||||||||||
Additional information
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| Size range | 1060-1499 amino acids |
| Related rules |
None |
| Fusion | Nter: None Cter: MF_01322 (rpoC) |
| Comments | Fused with rpoC in Helicobacter species and at least some Wolbachia, but not in other epsilon proteobateria. CHLVU, ODOSI and PSEAK have large inserts, HETCA has a deletion, CHLRE is split. None are used in the alignment. |