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HAMAP rule MF_01321

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General rule information [?]

Accession MF_01321
Dates 6-JAN-2004 (Created)
1-JUN-2023 (Last updated, Version 35)
Name RNApol_bact_RpoB
Scope
Bacteria
Plastid
Templates Q9KWU7 (RPOB_THEAQ); P0A8V2 (RPOB_ECOLI): [Recover all]
Triggered by

Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
RPOB
case not <OG:Chloroplast>
Protein name
RecName: Full=DNA-directed RNA polymerase subunit beta;
Short=RNAP subunit beta;
EC 2.7.7.6;
AltName: Full=RNA polymerase subunit beta;
AltName: Full=Transcriptase subunit beta;
else case <OG:Chloroplast>
Protein name
RecName: Full=DNA-directed RNA polymerase subunit beta;
EC 2.7.7.6;
AltName: Full=PEP;
AltName: Full=Plastid-encoded RNA polymerase subunit beta;
Short=RNA polymerase subunit beta;
end case
Gene name
rpoB

Comments [?]

Function DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.
Catalytic activity RHEA:21248: a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate + RNA(n+1)
EC 2.7.7.6
case not <OG:Chloroplast> and not <OC:Cyanobacteriota>
Subunit The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta' and 1 omega subunit. When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription.
else case <OC:Cyanobacteriota>
Subunit In cyanobacteria the RNAP catalytic core is composed of 2 alpha, 1 beta, 1 beta', 1 gamma and 1 omega subunit. When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription.
else case <OG:Chloroplast>
Subunit In plastids the minimal PEP RNA polymerase catalytic core is composed of four subunits: alpha, beta, beta', and beta''. When a (nuclear-encoded) sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription.
Subcellular location Plastid, chloroplast.
end case
Similarity Belongs to the RNA polymerase beta chain family.

Keywords [?]

case (<OC:Escherichia> or <OC:Shigella>) and (<FT:1> or <FT:2>)
Acetylation
end case
Transcription
Transferase
Nucleotidyltransferase
DNA-directed RNA polymerase

Gene Ontology [?]

GO:0003677; Molecular function: DNA binding.
GO:0003899; Molecular function: DNA-directed 5'-3' RNA polymerase activity.
GO:0006351; Biological process: DNA-templated transcription.
case <OG:Chloroplast>
GO:0009507; Cellular component: chloroplast.
end case

Cross-references [?]

Pfam PF04563; RNA_pol_Rpb2_1; 1;
PF04561; RNA_pol_Rpb2_2; 1;
PF04565; RNA_pol_Rpb2_3; 1;
PF00562; RNA_pol_Rpb2_6; 1;
PF04560; RNA_pol_Rpb2_7; 1;
NCBIfam TIGR02013; RpoB; 1;
PROSITE PS01166; RNA_POL_BETA; 1;

Features [?]

case <OC:Escherichia> or <OC:Shigella>
From: RPOB_ECOLI (P0A8V2)
Key     From     To       Description   Tag   Condition   FTGroup
MOD_RES     1022     1022       N6-acetyllysine     K  
MOD_RES     1200     1200       N6-acetyllysine     K  
end case

Additional information [?]

Size range 1060-1499 amino acids
Related rules None
Fusion Nter: None; Cter: MF_01322 (rpoC)
Comments Fused with rpoC in Helicobacter species and at least some Wolbachia, but not in other epsilon proteobateria. CHLVU, ODOSI and PSEAK have large inserts, HETCA has a deletion, CHLRE is split. None are used in the alignment.


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